Gene expression profiles of specific chicken skeletal muscles

Kui, Hua; Ran, Bo; Yang, Maosen; Shi, X.; Luo, Yingyu; Wang, Yujie; Wang, Tao; Li, Diyan; Shuai, Surong; Li, Mingzhou

doi:10.1038/s41597-022-01668-w

Cited by 8 publications

(7 citation statements)

References 17 publications

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“…pro/index) harbors the raw data obtained from wholegenome sequencing, RNA-seq, ChIP-seq, and ATACseq analyses of 21 animal species [15]; the Animal Imputation Database (https://ngdc.cncb.ac.cn/databasecommons/database/id/6833) is dedicated to collecting publicly available genomic sequencing data obtained from livestock animals to build high-quality reference panels and covers 13 species and has 2265 samples [16]. Additionally, the ISwine Database (http://iswine.iomics.pro/pig-iqgs/iqgs/index) [17], Ruminant Genome Database (http://222.90.83.22:88/ code/index.php/RGD) [18], Goat Genome Variation Database (http://animal.omics.pro:88/code/index.php/ GoatVar) [19], and other databases or datasets with genomic variation information [20][21][22], transcriptomic characteristics [23][24][25], genome functional annotation [26][27][28][29], and pan-genomes [30][31][32][33] are all contributing to the better understanding of the genetic basis of traits through GWAS.…”

Section: Genome-wide Association Study In Farm Animalsmentioning

confidence: 99%

Research progress and applications of genome‐wide association study in farm animals

Tan

Fahey

et al. 2023

Animal Research and One Health

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Exploring the genetic loci underlying economic traits is foundational for innovation in modern animal breeding technology. Genome‐wide association studies (GWAS) have been valid and commonly used tools to dissect the genomic variants associated with phenotypes for the past ∼20 years and have contributed to our understanding of genetic and molecular bases of various traits. Here, we comprehensively review the recent research progress on GWAS methods. We highlight the methodological advancements enabled by the combination of new proteome, transcriptome, epigenome, and metagenome information and multi‐omics analysis algorithms. The advances in GWAS investigations of chickens, pigs, cattle, and other animals during the last 5 years are also described. Finally, we discuss the current applications of GWAS in cutting‐edge breeding technologies and overall future perspectives of the post‐GWAS era.

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Section: Genome-wide Association Study In Farm Animalsmentioning

confidence: 99%

Research progress and applications of genome‐wide association study in farm animals

Tan

Fahey

et al. 2023

Animal Research and One Health

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“…The fibre type in chicken breast muscles is almost always type IIB, regardless of local and commercial breeds (Verdiglione & Cassandro, 2013 ). The leg muscle of chickens has a similar fibre type composition and is composed of more than 80% type IIB and less than 20% type I fibres (Kui et al., 2022 ). This difference in muscle fibre composition affects the meat quality, texture and tenderness of the final product.…”

Section: Muscle Fibre Characteristics and Imf Cont...mentioning

confidence: 99%

Emerging perspectives in the gut–muscle axis: The gut microbiota and its metabolites as important modulators of meat quality

Wen,

Wang,

et al. 2023

Microbial Biotechnology

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Animal breeding has made great genetic progress in increasing carcass weight and meat yield in recent decades. However, these improvements have come at the expense of meat quality. As the demand for meat quantity continues to rise, the meat industry faces the great challenge of maintaining and even increasing product quality. Recent research, including traditional statistical analyses and gut microbiota regulation research, has demonstrated that the gut microbiome exerts a considerable effect on meat quality, which has become increasingly intriguing in farm animals. Microbial metabolites play crucial roles as substrates or signalling factors to distant organs, influencing meat quality either beneficially or detrimentally. Interventions targeting the gut microbiota exhibit excellent potential as natural ways to foster the conversion of myofibres and promote intramuscular fat deposition. Here, we highlight the emerging roles of the gut microbiota in various dimensions of meat quality. We focus particularly on the effects of the gut microbiota and gut‐derived molecules on muscle fibre metabolism and intramuscular fat deposition and attempt to summarize the potential underlying mechanisms.

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“…1. 골격근 특이 발현 조절 프로모터 선정 골격근에서 특이적으로 발현하는 프로모터를 개발하기 위해, 우선 문헌 (Song et al, 2013;Raza et al, 2021;Kui et al, 2022)…”

Section: 재료 및 방법unclassified

Development of Promoters Inducing Gene Expression in Poultry Muscle Cells

Kang,

Nam,

Lee

et al. 2023

Korean J. Poult. Sci.

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The skeletal muscles of livestock play a crucial role as protein sources for humans, and the consumption of poultry meat is steadily increasing worldwide. Numerous genes, including myogenic regulatory factors, are involved in myogenesis, and precise regulation of them is essential. In this study, genes specifically expressed in muscles were selected, and their promoters were cloned and analyzed. The analysis of gene expression in various tissues of animals revealed that many genes exhibited specific expression patterns in skeletal muscles, with TNNT3, TNNC2, and MYF6 genes showing similar patterns in poultry. The promoter regions of three genes were amplified by polymerase chain reaction to sizes of 1.2 kb, 1.03 kb, and 1.43 kb, respectively. These fragments were then inserted at the front of the enhanced green fluorescent protein gene in vectors. It was confirmed that the sequences of three promoters closely matched the chicken genome sequences. Upon introducing vectors with each promoter into QM7 quail muscle cells, all three promoters successfully induced the expression of the green fluorescent protein. The brightness of the green fluorescence in each promoter was approximately seven times dimmer compared to the control, CMV-IE promoter. It is predicted that more than 230 transcription factors can bind to each promoter, especially various transcription factors expressed in muscles, including myogenic regulatory factors such as MYF5, MYOD, and MYOG. These promoters can be valuable for studying gene expression in poultry muscle cells, and further research is needed to precisely investigate the regulatory region of gene expression in promoters.

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Gene expression profiles of specific chicken skeletal muscles

Cited by 8 publications

References 17 publications

Research progress and applications of genome‐wide association study in farm animals

Research progress and applications of genome‐wide association study in farm animals

Emerging perspectives in the gut–muscle axis: The gut microbiota and its metabolites as important modulators of meat quality

Development of Promoters Inducing Gene Expression in Poultry Muscle Cells

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