Gene expression profiles of the small intestine of village chickens from an Ascaridia galli infested environment

Malatji, Dikeledi Petunia; Marle-Köster, E. van; Muchadeyi, Farai C.

doi:10.1016/j.vpoa.2019.100012

Cited by 6 publications

(11 citation statements)

References 49 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concentration of DNA was measured by a nanodrop spectrophotometer and PCR was performed. Partial fragments of the mitochondrial gene ( cox1 ) were amplified using the previously published primer sets ( Malatji et al, 2019 ). Briefly, PCR amplifications were performed in 25 μL reactions using One Taq Quick-Load 2X Master Mix containing ∼2.5 units of puReTaq DNA polymerase, 10 mM Tris–HCl (pH 9.0), 50 mM KCl, 1.5 mM MgCl 2 , 200 μM of each dNTP and stabilizers including BSA, 10 pmol of each PCR primer (Ag Cox1F: ATT ATT ACT GCT CAT GCT ATT TTG ATG and Ag Cox1R: CAA AAC AAA TGT TGA TAA ATC AAA GG), and 50 ng of genomic DNA.…”

Section: Methodsmentioning

confidence: 99%

Ascaridia galli, a common nematode in semiscavenging indigenous chickens in Bangladesh: epidemiology, genetic diversity, pathobiology, ex vivo culture, and anthelmintic efficacy

Ritu,

Labony,

Hossain

et al. 2024

Poultry Science

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Ascaridia galli, a common nematode in semiscavenging indigenous chickens in Bangladesh: epidemiology, genetic diversity, pathobiology, ex vivo culture, and anthelmintic efficacy

Ritu,

Labony,

Hossain

et al. 2024

Poultry Science

View full text Add to dashboard Cite

“…The pathogenesis of A. galli in chickens is mostly associated with hypertrophy of the intestinal villi infiltration of inflammatory cells, especially eosinophils, lymphocytes, and macrophages. Necrosis of the crypts of Lieberkühn is associated with the histotrophic phase of larvae 24 . Infection can result in poor growth followed by poor performance 45 .…”

Section: Transmission and Pathobiologymentioning

confidence: 99%

“…22,23 The intestinal epithelium acts as a communication network for this gut-dwelling nematode as it transmits signals to the immune systems (innate and acquired) in the underlying mucosa. 24 Macroscopically, A. galli infection includes a thickened intestinal wall with hemorrhagic spots along with edema and infiltration of lymphoid cells mixed with eosinophils. 25 Occasionally, ulcerative proventriculitis can also be detected in ascaridiasis.…”

Section: Introductionmentioning

confidence: 99%

Ascaridia galli infection in chicken: Pathobiology and immunological orchestra

Shohana,

Rony,

Ali

et al. 2023

Immunity Inflam & Disease

View full text Add to dashboard Cite

BackgroundAscaridia galli is the largest gut‐dwelling helminth of chickens, which confers adverse effects on meat and egg production; thus, on the animal protein supply and the economy. Both adult and immature parasites affect gut health, but larval stages play a major role in pathology.AimsHere, we present immunology and pathology of A. galli in chickens.Materials and MethodsLiteratures were surveyed through online platforms such as PubMed, Google Scholar and Researchgate.ResultsThe larvae cause excessive mucus production, damage to the intestinal gland, hemorrhage, anemia, diarrhea, and malnutrition. The adult worms can cause death by intestinal obstruction and intussusception. Although both cellular and humoral immunity are involved in fighting against ascariasis, the role of naturally acquired immunity is poorly defined. In cellular immunity, Th‐2 cytokines (IL‐4, IL‐5, IL‐9, and IL‐13), goblet cells (mucin), gut‐associated lymphoid tissues, CD8α+ intraepithelial cells, TCRγδ + T cells, and TGF‐β4 form a protective band. Type 2 immunity provides protection by forming a network of endogenous damage‐associated molecular patterns, chitin, and parasitic antigens. Among antibodies, IgY is the most prominent in chickens and provides temporary humoral protection. During parasitic infection, infiltration of various immune cells is evident, especially in the intestinal epithelium, lamina propria, and crypts of the duodenum and jejunum. In chickens older than 12 weeks, gradual reduction of worm burden is more successful than the younger birds. Female chickens exert a short‐lived but higher level of protection by passing IgY to chicks in the form of egg yolk antibodies. In laying conditions, immunity differs between breeds. This review provides an overview of the silent but inevitable pathological changes induced by A. galli and the interaction of host immunity with the parasite.

show abstract

“…The pathogenesis of A. galli in chickens is mostly associated with hypertrophy of the intestinal villi, infiltration of inflammatory cells, especially eosinophils, lymphocytes, and macrophages. Necrosis of the crypts of Lieberkühn is associated with the histotrophic phase of larvae [21]. Infection results in poor growth followed by poor performance [41].…”

Section: Transmission and Pathobiologymentioning

confidence: 99%

“…The intestinal epithelium acts as a communication network for this gut dwelling nematode as it transmits signals to the immune systems (innate and acquired) in the underlying mucosa [21]. Macroscopically,A.…”

Section: Introductionmentioning

confidence: 99%

Ascaridia galli infection in chicken: Pathobiology and Immunological orchestra

Anisuzzaman

Shohana

Rony

et al. 2023

Preprint

View full text Add to dashboard Cite

Ascaridia galli is the largest gut-dwelling helminth of chickens which confers adverse effects on meat and egg production and thus, on the animal protein supply and the economy. Both adult and immature parasites affect the gut health, but larval stages play the major role in pathology. The larvae cause excessive mucus production, damage to the intestinal gland, hemorrhage, anemia, diarrhea, and malnutrition. The adult worms can cause death by intestinal obstruction and intussusception. Although both cellular and humoral immunity are involved in fighting against ascaridiasis, the role of naturally acquired immunity is poorly defined. In cellular immunity, Th-2 cytokines (IL-4, IL-5, IL-9 and IL-13), goblet cells (mucin), GALTs (gut -associated lymphoid tissues), CD8α+ intraepithelial cells, TCRγδ+T cells, and TGF-β4 form a protective band. Type 2 immunity provides protection by forming a network of eDAMP (endogenous damage-associated molecular pattern), chitin, and parasitic antigens. Among antibodies, IgY is the most prominent in chickens and provides temporary humoral protection. During parasitic infection, infiltration of various immune cells is evident, especially in the intestinal epithelium, lamina propria, and crypts of the duodenum and jejunum. In chickens older than 3 months, gradual reduction of worm burden is more successful. Female chickens exert a short-lived but higher level of protection by passing IgY to chicks in the form of egg yolk antibodies (EAB). In laying condition, immunity differs greatly between breeds. This review provides a good overview of the silent but inevitable pathological changes induced by A. galli; and the interaction of host immunity with the parasite.

show abstract

Gene expression profiles of the small intestine of village chickens from an Ascaridia galli infested environment

Cited by 6 publications

References 49 publications

Ascaridia galli, a common nematode in semiscavenging indigenous chickens in Bangladesh: epidemiology, genetic diversity, pathobiology, ex vivo culture, and anthelmintic efficacy

Ascaridia galli, a common nematode in semiscavenging indigenous chickens in Bangladesh: epidemiology, genetic diversity, pathobiology, ex vivo culture, and anthelmintic efficacy

Ascaridia galli infection in chicken: Pathobiology and immunological orchestra

Ascaridia galli infection in chicken: Pathobiology and Immunological orchestra

Contact Info

Product

Resources

About