2006
DOI: 10.1152/physiolgenomics.00090.2006
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Gene expression profiling in the neuroendocrine brain of male goldfish (Carassius auratus) exposed to 17α-ethinylestradiol

Abstract: 17-alpha ethinylestradiol (EE2), a pharmaceutical estrogen, is detectable in water systems worldwide. Although studies report on the effects of xenoestrogens in tissues such as liver and gonad, few studies to date have investigated the effects of EE2 in the vertebrate brain at a large scale. The purpose of this study was to develop a goldfish brain-enriched cDNA array and use this in conjunction with a mixed tissue carp microarray to study the genomic response to EE2 in the brain. Gonad-intact male goldfish we… Show more

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Cited by 77 publications
(58 citation statements)
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“…We used pooled samples that were used for microarray analysis (n ϭ 3) and additional independent samples (n ϭ 7) for the SYBR Green assay. Each PCR reaction was run with parameters described by Martyniuk and colleagues (34), with primer concentrations of 150 nM for all genes validated. Primers (Table 1) were designed with Primer3 and ordered from Invitrogen.…”
Section: Methodsmentioning
confidence: 99%
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“…We used pooled samples that were used for microarray analysis (n ϭ 3) and additional independent samples (n ϭ 7) for the SYBR Green assay. Each PCR reaction was run with parameters described by Martyniuk and colleagues (34), with primer concentrations of 150 nM for all genes validated. Primers (Table 1) were designed with Primer3 and ordered from Invitrogen.…”
Section: Methodsmentioning
confidence: 99%
“…We previously described and validated the production and use of our goldfish-carp cDNA microarray (33,34). For a detailed description of the microarray the reader is directed to Williams et al (68).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The quality and quantity of sequence data generated by rapidly evolving sequencing techniques revolutionized research with non-model species, allowing researchers to construct high-quality microarrays for relevant species instead of focusing research efforts in model species. Such is the case of the development of a largemouth bass microarray (Garcia-Reyero et al, 2008), used to study estrogenic response in males; or a goldfish cDNA array (Martyniuk et al, 2006), used to study estrogenic effects on neuroendocrine function. The development of microarrays for non-model species though often faces the problem of poorly of annotated probes.…”
Section: Omics and Vertebrate Non-model Speciesmentioning
confidence: 99%
“…Relatively straightforward applications have addressed what genes are responsive to a toxicant, and discriminate between those with known modes of action, leading to more in-depth hypotheses regarding molecular mechanisms underlying potential adverse effects [14][15][16][17][18][19]. Microarrays assess impacts on many different genes involved in many different processes and functions, therefore are well suited for the evaluation of the potentially complex effects of chemical mixtures.…”
mentioning
confidence: 99%