Gene expression profiling of cutaneous wound healing

Deonarine, Kavita; Panelli, Monica C.; Stashower, Mitchell E.; Jin, Ping; Smith, Kina; Slade, Herbert B.; Norwood, Christopher; Wang, Ena; Marincola, Francesco M.; Stroncek, David F.

doi:10.1186/1479-5876-5-11

Cited by 188 publications

(88 citation statements)

References 29 publications

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“…The macrophage population present immediately following tissue injury possesses predominantly M1 characteristics [20, 21]. Transition to an M2 phenotype occurs concurrently with resolution of the inflammatory process and the initiation of the remodeling phase of wound healing [20].…”

Section: 0 Introductionmentioning

confidence: 99%

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Macrophage phenotype as a predictor of constructive remodeling following the implantation of biologically derived surgical mesh materials

et al. 2012

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Macrophages have been classified as having plastic phenotypes which exist within a spectrum between M1 (classically activated; pro-inflammatory) and M2 (alternatively activated; regulatory, homeostatic). To date, the effects of polarization towards a predominantly M1 or M2 phenotype have been studied largely in the context of response to pathogen or cancer. Recently, M1 and M2 macrophages have been shown to play distinct roles in tissue remodeling following injury. In the present study, the M1/M2 paradigm was utilized to examine the role of macrophages in the remodeling process following implantation of 14 biologically derived surgical mesh materials in the rat abdominal wall. In situ polarization of macrophages responding to the materials was examined and correlated to a quantitative measure of the observed tissue remodeling response to determine whether macrophage polarization is an accurate predictor of the ability of a biologic scaffold to promote constructive tissue remodeling. Additionally the ability of M1 and M2 macrophages to differentially recruit progenitor-like cells in vitro, which are commonly observed to participate in the remodeling of those ECM scaffolds which have a positive clinical outcome, was examined as a possible mechanism underlying the differences in the observed remodeling responses. The results of the present study show that there is a strong correlation between the early macrophage response to implanted materials and the outcome of tissue remodeling. Increased numbers of M2 macrophages and higher ratios of M2:M1 macrophages within the site of remodeling at 14 days were associated with more positive remodeling outcomes (r2=0.525–0.686, p<0.05). Further, the results of the present study suggest that the constructive remodeling outcome may be due to the recruitment and survival of different cell populations to the sites of remodeling associated with materials that elicit an M1 versus M2 response. Both M2 and M0 macrophage conditioned medias were shown to have higher chemotactic activities than media conditioned by M1 macrophages (p<0.05). A more thorough understanding of these issues will logically influence the design of next generation biomaterials and the development of regenerative medicine strategies for the formation of functional host tissues.

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Section: 0 Introductionmentioning

confidence: 99%

“…Transition to an M2 phenotype occurs concurrently with resolution of the inflammatory process and the initiation of the remodeling phase of wound healing [20]. This process represents the default mammalian host response to tissue injury, and generally results in the formation of localized scar tissue.…”

Section: 0 Introductionmentioning

confidence: 99%

Macrophage phenotype as a predictor of constructive remodeling following the implantation of biologically derived surgical mesh materials

et al. 2012

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“…A comprehensive genetic study mapping the gene expression profiles during the cutaneous wound healing has motivated the development of gene delivery strategies in facilitating wound healing (Deonarine et al, 2007). Biomaterial-based nucleic acid delivery systems reduce degradation, enhance uptake, and control the treatment dose.…”

Section: Biomaterial-based Wound Therapiesmentioning

confidence: 99%

Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing

Baker

2016

Front. Bioeng. Biotechnol.

254

196

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Wound healing is an intricate process that requires complex coordination between many cell types and an appropriate extracellular microenvironment. Chronic wounds often suffer from high protease activity, persistent infection, excess inflammation, and hypoxia. While there has been intense investigation to find new methods to improve cutaneous wound care, the management of chronic wounds, burns, and skin wound infection remain challenging clinical problems. Ideally, advanced wound dressings can provide enhanced healing and bridge the gaps in the healing processes that prevent chronic wounds from healing. These technologies have great potential for improving outcomes in patients with poorly healing wounds but face significant barriers in addressing the heterogeneity and clinical complexity of chronic or severe wounds. Active wound dressings aim to enhance the natural healing process and work to counter many aspects that plague poorly healing wounds, including excessive inflammation, ischemia, scarring, and wound infection. This review paper discusses recent advances in the development of biomaterials and nanoparticle therapeutics to enhance wound healing. In particular, this review focuses on the novel cutaneous wound treatments that have undergone significant preclinical development or are currently used in clinical practice.

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“…If the genetic contribution to proper palatogenesis is unquestionable, and the list of genes involved in cleft lip and palate established at about 300 (Jugessur et al, 2009), it has been challenging to generate a workable list of genes involved in wound healing, mainly because of the complexity of the process and the difficulties of obtaining relevant human samples. Nevertheless, a few studies have performed gene expression profiling of cutaneous wound healing (Deonarine et al, 2007; Roupe et al, 2010; Nuutila et al, 2012), or identified a genetic signature unique to non-healing wounds (Charles et al, 2008), yet none provided access to the full list of differentially-regulated genes. Using available information from these studies, we estimated the percentage of reported wound-healing genes that shared a role in CL/P.…”

Section: When Things Go Wrongmentioning

confidence: 99%

Palatogenesis and cutaneous repair: A two‐headed coin

Biggs

Goudy

Dunnwald

2014

Developmental Dynamics

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The reparative mechanism that operates following post-natal cutaneous injury is a fundamental survival function that requires a well-orchestrated series of molecular and cellular events. At the end, the body will have closed the hole using processes like cellular proliferation, migration, differentiation and fusion. These processes are similar to those occurring during embryogenesis and tissue morphogenesis. Palatogenesis, the formation of the palate from two independent palatal shelves growing towards each other and fusing, intuitively, shares many similarities with the closure of a cutaneous wound from the two migrating epithelial fronts. In this review, we summarize the current information on cutaneous development, wound healing, palatogenesis and orofacial clefting and propose that orofacial clefting and wound healing are conserved processes that share common pathways and gene regulatory networks.

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Gene expression profiling of cutaneous wound healing

Cited by 188 publications

References 29 publications

Macrophage phenotype as a predictor of constructive remodeling following the implantation of biologically derived surgical mesh materials

Macrophage phenotype as a predictor of constructive remodeling following the implantation of biologically derived surgical mesh materials

Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing

Palatogenesis and cutaneous repair: A two‐headed coin

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