Gene expression profiling of ovine keratinocytes stimulated with <i>Psoroptes ovis</i> mite antigen – a preliminary study

Watkins, Craig; Mackellar, A.; Frew, David; Mackie, Carol; George, Abraham; Hopkins, John; Burgess, Stewart T. G.; McNeilly, Tom N.; Huntley, John F.

doi:10.1111/j.1365-3024.2009.01103.x

Cited by 12 publications

(12 citation statements)

References 25 publications

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“…Increased IL8 expression supports our previous findings from ovine keratinocyte cultures stimulated with P. ovis mite wash and whole mite extract [14]. However, the timing of this response differs between the in vitro and in vivo systems as the previous study had shown that IL8 expression levels began to decline by 24 hpi; whereas this time point represented the peak level in vivo [14]. However, it cannot be ruled out that this response continues to be activated beyond 24 hpi.…”

Section: Resultssupporting

confidence: 91%

“…IL8 is a potent chemo-attractant and angiogenic factor involved in the chemotaxis of neutrophils, basophils and T-cells, and has also been shown to be involved in eosinophil chemotaxis [96]. Increased IL8 expression supports our previous findings from ovine keratinocyte cultures stimulated with P. ovis mite wash and whole mite extract [14]. However, the timing of this response differs between the in vitro and in vivo systems as the previous study had shown that IL8 expression levels began to decline by 24 hpi; whereas this time point represented the peak level in vivo [14].…”

Section: Resultssupporting

confidence: 79%

“…This finding has implications for sheep scab which is characterised by high levels of the pro-inflammatory cytokine IL8 [14], and it is plausible that IL8 and regakine could work synergistically with other cytokines to enhance the inflammatory response to infestation with P. ovis . In addition, as described for cluster 6 (below), the HDM derived allergen Der p 3 cleaves complement components C3 and C5, producing the anaphylatoxins C3a and C5a, respectively [99].…”

Section: Resultsmentioning

confidence: 99%

“…Our group recently demonstrated the up-regulation of the pro-inflammatory factor interleukin-8 ( IL8 ) in ovine keratinocytes within 1 hour of P. ovis exposure, supporting the rapidity and pro-inflammatory nature of the host response [14]. Here, in order to characterise the host skin response during the first 24 hours post-infestation we employed an ovine transcriptome microarray.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

et al. 2010

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BackgroundInfestation of ovine skin with the ectoparasitic mite Psoroptes ovis results in a rapid cutaneous immune response, leading to the crusted skin lesions characteristic of sheep scab. Little is known regarding the mechanisms by which such a profound inflammatory response is instigated and to identify novel vaccine and drug targets a better understanding of the host-parasite relationship is essential. The main objective of this study was to perform a combined network and pathway analysis of the in vivo skin response to infestation with P. ovis to gain a clearer understanding of the mechanisms and signalling pathways involved.ResultsInfestation with P. ovis resulted in differential expression of 1,552 genes over a 24 hour time course. Clustering by peak gene expression enabled classification of genes into temporally related groupings. Network and pathway analysis of clusters identified key signalling pathways involved in the host response to infestation. The analysis implicated a number of genes with roles in allergy and inflammation, including pro-inflammatory cytokines (IL1A, IL1B, IL6, IL8 and TNF) and factors involved in immune cell activation and recruitment (SELE, SELL, SELP, ICAM1, CSF2, CSF3, CCL2 and CXCL2). The analysis also highlighted the influence of the transcription factors NF-kB and AP-1 in the early pro-inflammatory response, and demonstrated a bias towards a Th2 type immune response.ConclusionsThis study has provided novel insights into the signalling mechanisms leading to the development of a pro-inflammatory response in sheep scab, whilst providing crucial information regarding the nature of mite factors that may trigger this response. It has enabled the elucidation of the temporal patterns by which the immune system is regulated following exposure to P. ovis, providing novel insights into the mechanisms underlying lesion development. This study has improved our existing knowledge of the host response to P. ovis, including the identification of key parallels between sheep scab and other inflammatory skin disorders and the identification of potential targets for disease control.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

et al. 2010

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“…The first approach to investigate the early inflammatory response, was to generate ovine primary keratinocyte cultures challenged with mite derived antigens, and the kinetics of the mRNA response of these cells were monitored by microarray. Authors found that six genes were up-regulated, showing a significant increase in the pro-inflammatory cytokine IL-8 [39]. Afterwards, it was characterized the inflammatory response from sheep skin biopsy samples, achieved through microarrays and qRT-PCR, founding an up-regulation of transcripts for pro-inflammatory cytokine genes IL-1β, IL-6 and IL-8.…”

Section: Discussionmentioning

confidence: 99%

Hormonal and behavioral changes induced by acute and chronic experimental infestation with Psoroptes cuniculi in the domestic rabbit Oryctolagus cuniculus

et al. 2013

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BackgroundParasitic diseases are important in animal production because they cause high economic losses. Affected animals often exhibit stereotypical behavioral alterations such as anorexia and inactivity, among others. Among the diseases that commonly affect domestic rabbits is mange, which is caused by the mite Psoroptes cuniculi. Therefore, within the context of the host-parasite relationship, it is critical to understand the mechanisms involved in the alteration of host behavior, in order to better utilize sick animal behavior as a strategy for diagnosis and treatment of disease.MethodsRabbits were infested placing mites in the ear conduct. We characterized changes in exploratory behavior and scent marking evoked by acute (1-9 days) and chronic (25-33 days) experimental infestation. Behavior was recorded during ten minutes while the animals were in a 120 cm × 120 cm open field arena divided into 9 squares. Serum cortisol was measured individually using radioimmunoassay kits. Locomotor activity, chinning, rearing and body weight were compared using a Friedman test, the effect of treatment (infested versus non-infested) across time was analyzed using a repeated measures ANOVA, and the Pearson test was used to determine whether chinning and ambulation scores were significantly correlated. Serum cortisol levels and food consumption were analyzed with a Kruskal-Wallis test and body temperature was analyzed with an ANOVA test.ResultsWe observed a significant decrease in rearing behavior as early as two days post-infestation, while chinning and locomotor activity were significantly decreased four days post-infestation. Chronic infestation was associated with decreased food intake, significant weight loss, and a trend toward increased serum cortisol levels, while no changes were observed in body temperature.ConclusionsThe presence of visible lesions within the ear canal is commonly used to detect mite infestation in rabbits, but this is possible only after chronic infestation. The behaviors described here may be a useful and economic tool in guiding the early diagnosis of parasitic infestation by P. cuniculi, allowing for early treatment and the application of control measures before significant weight loss occurs, thereby avoiding economic losses.

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Immunopathogenesis of Allergic Skin Disease in Livestock

Broek

2013

Veterinary Allergy

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Gene expression profiling of ovine keratinocytes stimulated with Psoroptes ovis mite antigen – a preliminary study

Cited by 12 publications

References 25 publications

Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

Hormonal and behavioral changes induced by acute and chronic experimental infestation with Psoroptes cuniculi in the domestic rabbit Oryctolagus cuniculus

Immunopathogenesis of Allergic Skin Disease in Livestock

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