2015
DOI: 10.1186/s13567-015-0178-z
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Gene expression profiling of porcine mammary epithelial cells after challenge with Escherichia coli and Staphylococcus aureus in vitro

Abstract: Postpartum Dysgalactia Syndrome (PDS) represents a considerable health problem of postpartum sows, primarily indicated by mastitis and lactation failure. The poorly understood etiology of this multifactorial disease necessitates the use of the porcine mammary epithelial cell (PMEC) model to identify how and to what extent molecular pathogen defense mechanisms prevent bacterial infections at the first cellular barrier of the gland. PMEC were isolated from three lactating sows and challenged with heat-inactivate… Show more

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Cited by 22 publications
(40 citation statements)
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“…Early response of PMECs at 3 hpc with E. coli was followed by a late, more intensive host response at 24 hpc, as indicated by an approximately two-fold increase of significantly DE miRNAs. This is in accordance with our mRNA microarray analysis, which also identified a more intense host response after long-term challenge with E. coli [ 7 ].…”
Section: Discussionsupporting
confidence: 91%
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“…Early response of PMECs at 3 hpc with E. coli was followed by a late, more intensive host response at 24 hpc, as indicated by an approximately two-fold increase of significantly DE miRNAs. This is in accordance with our mRNA microarray analysis, which also identified a more intense host response after long-term challenge with E. coli [ 7 ].…”
Section: Discussionsupporting
confidence: 91%
“…Pairwise correlation coefficient analysis was performed to evaluate associations of expression levels between 442 significantly DE miRNA probesets and 1345 significantly DE mRNA probesets (previously identified by Jaeger et al [ 7 ]). In total, 36,610 miRNA–mRNA pairs were significantly negatively correlated ( p < 0.05) and in silico predicted as functionally linked.…”
Section: Resultsmentioning
confidence: 99%
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