Gene expression profiling on effect of aspirin on osteogenic differentiation of periodontal ligament stem cells

Abstract

Periodontal ligament (PDL) contains a unique population of mesenchymal stem cells (MSCs), also known as PDL stem cells (PDLSCs). The regenerative properties of PDLSCs hold great potential for its use in stem cells based therapy, particularly for periodontal or bone regeneration. The present study investigated the global gene expression profile in PDLSCs during osteogenic differentiation. MSCs from PDL were isolated from normal permanent human teeth (n = 3). Microarray analysis was used to study the effects of ASA (200, 500, and 1000 μM) on the gene expression profiles in PDLSCs during osteogenic differentiation. Microarray study revealed that ASA was able to modulate PDLSCs gene expression profile. At 200 µM, 315 genes were dysregulated genes (DE), involving 151 upregulated and 164 downregulated genes. At 500 µM, 794 genes were DE, involving of 364 upregulated and 430 downregulated genes. At 1000 µM, the number of DE genes increased to 2035, of which 735 were upregulated and 1300 were downregulated. Bioinformatics analyses of the gene expression data revealed that the majority of DE genes (for 500 and 1000 µM ASA treatment) are involved in osteogenic differentiation. The gene network analysis was carried out using Ingenuity Pathway Analysis (IPA) software, and this revealed that the number of gene groups involved in cell adhesion and extracellular matrix components were increased. This study indicated that ASA could enhance PDLSCs functions and provide evidence for the potential use of ASA with PDLSCs for regenerative dentistry applications, particularly in the areas of periodontal health and regeneration. Periodontal ligament stem cells (PDLSCs) Aspirin (ASA) Microarray Osteogenic

BDJ Open 2021 - our most successful year to date

“…There were just four papers published reporting the results of ex vivo studies. 15,16,17,18 These outline early pre-clinical studies of new health technologies (new materials, methods and pharmacological interventions) and in vitro studies of existing technologies. These were relatively more frequent in the early days of BDJ Open, but this journal now covers the whole range of dental research.…”

Transcriptomic landscape of human induced pluripotent stem cell-derived osteogenic differentiation identifies a regulatory role of KLF16

“…For instance, lncRNA H19 promotes osteoblast differentiation via the TGF-β1/SMAD3/HDAC pathway by encoding miR-675 (Huang et al, 2015), while lncRNA MALAT1 enhances osteoblast differentiation through SMAD4 up-regulation by sequestering miR-204 (Xiao et al, 2017). Numerous studies have independently investigated both human coding and lncRNA gene expression profiles during osteogenic differentiation (Abd Rahman, 2021; Håkelien et al, 2014; Hong et al, 2020; Huang et al, 2017; Khodabandehloo et al, 2020; Liu et al, 2020; Park et al, 2015; Qiu et al, 2017; Shaik et al, 2019; Skårn et al, 2014; Song et al, 2015; Sun et al, 2019; Twine et al, 2014; Wang et al, 2015; Xia et al, 2020; Zhang et al, 2017; Zheng et al, 2018). However, many of these studies are limited by such factors as reliance on microarrays, small sample sizes, and brief osteogenic differentiation periods.…”

Formulation and Evaluation of Aspirin-PLGA Microsphere for the Dental Stem Cell Stimulation

“…8 Recent studies reported that Aspirin is capable of stimulating the various dental stem cells, this promotes the migration, proliferation, and differentiation of odontoblast cells responsible for the enhance the regeneration of dentin. [9][10][11] These findings strongly suggest that Aspirin has excellent potential in the management of several dental problems for which there is no clear-cut treatment strategy. Hence, Aspirin's therapeutic effectiveness depends on the design and development of a suitable novel drug delivery system for Aspirin.…”