Gene expression profiling reveals the defining features of the classical, intermediate, and nonclassical human monocyte subsets

Wong, Kelvin; Tai, June Jing Yi; Wong, William; Hao, Han; Sem, Xiaohui; Yeap, Wei Hseun; Kourilsky, Philippe; Wong, Siew Cheng

doi:10.1182/blood-2010-12-326355

Cited by 926 publications

(1,131 citation statements)

References 49 publications

Supporting

Mentioning

1,033

Contrasting

Unclassified

Order By: Relevance

“…11,12 Highest TNFa expression was found in non-classical monocytes, consistent with a strong pro-inflammatory potential. HLA-DR and CCR5 were both expressed at highest levels in the intermediate subset, indicating a pronounced role in antigen-presentation as well as chemotactic response to tumor signals, respectively.…”

Section: Discussionmentioning

confidence: 67%

“…Therefore, their role in malignant disease seems ambiguous, as this subset is capable of stimulating tumor angiogenesis on the one hand, but may also exert antitumor effects on the other hand by the production of pro-inflammatory cytokines such as TNF-a, IL-1b and IL-6 and by antigen presentation. 11,24 In the present study with mCRC patients, intermediate monocytes in blood seem to reflect a systemic immunological response to chemotherapy. Thus, their increase in circulation may be induced by a systemic spillover of mediators released by the therapeutically damaged tumor tissue.…”

Section: Discussionmentioning

confidence: 99%

“…10 Recent studies on the gene expression profile of monocyte subsets have suggested a developmental relationship between these populations with gradual changes in surface markers during maturation. 11,12 In accordance, the non-classical subset may represent the most progressed stage of differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…Candidate proteins were again selected based on a former gene expression study showing divergent transcript levels in monocyte subsets for MMP-25 (MT6-MMP) and TIMP-1. 11 Furthermore, the tumor promoter and matrix-degrading protease cathepsin D (CTSD) was investigated, as it has previously been reported to be induced upon monocyte maturation, 19,20 and MMP-14 (MT1-MMP) was included, since it has been specifically connected to monocyte trans-endothelial migration. 21 None of the analyzed proteases showed a preferential expression in intermediate monocytes of CRC patients prior to treatment (Fig.…”

Section: Neoadjuvant Therapy Triggers a Significant Increase In Intermentioning

confidence: 99%

“…11,12 Non-classical monocytes (5%) exhibit a "patrolling" behavior i.e. crawling along vessel walls to screen for integrity 13 and they react strongly to viral signals.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response

et al. 2016

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Neoadjuvant Therapy Triggers a Significant Increase In Intermentioning

confidence: 99%

“…11,12 Non-classical monocytes (5%) exhibit a "patrolling" behavior i.e. crawling along vessel walls to screen for integrity 13 and they react strongly to viral signals.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response

et al. 2016

View full text Add to dashboard Cite

Phagocyte Development

Patel

Yona

2018

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Mature circulating white blood cells can be divided into two lineages: lymphoid and myeloid. The lymphoid lineage consists of T, B, NK and innate lymphoid cells, while the myeloid lineage includes functionally and morphologically distinct cell types including mononuclear phagocytes (monocytes, dendritic cells and macrophages), granulocytes (neutrophils, basophils, mast cells and eosinophils) and platelets. Granulocytes and mononuclear phagocytes are key players at all stages of the immune response from its genesis to its resolution, and come under the banner of phagocytes. Damaged or infected tissue releases chemoattractants that rapidly recruit these phagocytes to the site of injury. Once at the inflamed site, these cells coordinate and carry out immune functions, playing a key role in host defence. The recent past has seen major progress in our understanding of phagocyte developmental biology. Here, we discuss the latest advances in myeloid development, underpinning our current understanding of how these cells are generated and maintained. Key Concepts The myeloid lineage includes functionally and morphologically distinct cell types including mononuclear phagocytes (monocytes, dendritic cells and macrophages) and granulocytes (neutrophils, basophils, mast cells and eosinophils). The majority of tissue resident macrophages are generated before birth and are long‐lived self‐maintained cells throughout adulthood. Dendritic cells prime naïve T cells and are derived from a common precursor in the bone marrow that gives rise exclusively to this family of cells. Monocytes are the principal circulating mononuclear phagocyte. Monocytes develop in the bone marrow from a common monocyte progenitor, once in the circulation classical monocytes have the potential to convert into nonclassical monocytes. Classical monocytes are known to repopulate resident mononuclear phagocyte populations and have potent effector functions during immunity. Granulocyte subsets have a complex morphology with a segmented nucleus and each subset contains granules endowing them with a specific role in host defence. Transcription and growth factors control and dictate the development of phagocytes.

show abstract

Platelet–Monocyte Aggregate Instigates Inflammation and Vasculopathy in Kawasaki Disease

Zhang,

Jia,

Guo

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Kawasaki disease (KD) is a severe acute febrile illness and systemic vasculitis that causes coronary artery aneurysms in young children. Platelet hyperreactivity and an aberrant immune response are key indicators of KD; however, the mechanism by which hyperactive platelets contribute to inflammation and vasculopathy in KD remains unclear. A cytokine‐mediated positive feedback loop between KD platelets and monocytes is identified. KD platelet–monocyte aggregates (MPAs) are mediated by an initial interaction of P‐selectin (cluster of differentiation 62P, CD62p) and its glycoprotein ligand 1 (PSGL‐1). This is followed by a coordinated interaction of platelet glycoprotein (GP)Ibα with monocyte CD11b. Monocyte‐activated platelets initiate transforming growth factor (TGF)β1 release, which results in nuclear localization of nuclear factor kappaB in monocytes, therefore, driving the phenotypic conversion of classical monocytes (CD14+CD16−) into proinflammatory monocytes (CD14+CD16+). The platelet‐activated monocytes release interleukin‐1 and tissue necrotic factor‐α, which promote further platelet activation. KD‐induced inflammation and vasculopathy are prevented by inhibiting the components of this positive feedback loop. Notably, mice deficient in platelet TGFβ1 show less MPA and CD14+CD16+ monocytes, along with reduced inflammation and vasculopathy. These findings reveal that platelet–monocyte interactive proteins (CD62p/PSGL‐1 and (GP)Ibα/CD11b) and cytokine mediators (platelet TGFβ1) are potential biomarkers and therapeutic targets for KD vasculopathy.

show abstract

Gene expression profiling reveals the defining features of the classical, intermediate, and nonclassical human monocyte subsets

Cited by 926 publications

References 49 publications

Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response

Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response

Phagocyte Development

Platelet–Monocyte Aggregate Instigates Inflammation and Vasculopathy in Kawasaki Disease

Contact Info

Product

Resources

About