Gene expression profiling under different photoperiod/temperature conditions in a photoperiod-/thermo-sensitive genic male sterile line of rice (Oryza sativa L.)

Guo, Xin; Dong, Hua; Kangle, Zheng; Luo, Hongmei; Tan, Xiaofeng; Fang, Yongqi; Wang, Yanqin; Deng, Ye; Dai, Chengen; Lou, Yiting; Shao, Jing; Shi, Wenqin; Zhang, Dong; Li, Debao

doi:10.1007/s11434-005-1087-8

Cited by 6 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, LOC_Os07g 12130 that may be regarded as a member of a signal transduction pathway affects anther development by regulating gene expression at different temperatures. In young panicles of Pei′ai64S, Myb transcription factors showed a significantly lower transcriptional abundance under the LD/HT than under the SD/LT conditions, which may lead to a significant gene inhibition in the normal process of transcription (Guo et al, 2006).…”

Section: Correlation Between Myb-like Protein and Ptgms Genementioning

confidence: 94%

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Zhou

Xianyin

Xue

2011

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Pei′ai64S, an indica sterile variety with photoperiod and thermo-sensitive genic male sterile (PTGMS) genes, has been widely exploited for commercial seed production for "two-line" hybrid rice in China. One PTGMS gene from Pei′ai64S, pms1(t), was mapped by a strategy of bulked-extreme and recessive-class approach with simple sequence repeat (SSR) and insert and deletion (In-Del) markers. Using linkage analysis for the F 2 mapping population consisting of 320 completely male sterile individuals derived from a cross between Pei′ai64S and 93-11 (indica restorer) lines, the pms1(t) gene was delimited to the region between the RM21242 (0.2 cM) and YF11 (0.2 cM) markers on the short arm of chromosome 7. The interval containing the pms1(t) locus, which was co-segregated with RM6776, is a 101.1 kb region based on the Nipponbare rice genome. Fourteen predicted loci were found in this region by the Institute for Genomic Research (TIGR) Genomic Annotation. Based on the function of the locus LOC_Os07g12130 by bioinformatics analysis, it is predicted to encode a protein containing a Myb-like DNA-binding domain, and may process the transcript with thermosensory response. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the mRNA levels of LOC_Os07g12130 were altered in different photoperiod and temperature treatments. Thus, the LOC_Os07g12130 locus is the most likely candidate gene for pms1(t). These results may facilitate not only using the molecular marker assisted selection of PTGMS genes, but also cloning of the pms1(t) gene itself.

show abstract

Section: Correlation Between Myb-like Protein and Ptgms Genementioning

confidence: 94%

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Zhou

Xianyin

Xue

2011

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

show abstract

“…Description of preparation, analysis and quality control of cDNA array can be found in the published papers of our laboratory (Deng et al, 2006;Dong et al, 2005;Guo et al, 2005).…”

Section: Quality Controlmentioning

confidence: 99%

Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea

Jin

Dong

You-liang

et al. 2007

J. Zhejiang Univ. - Sci. B

Self Cite

View full text Add to dashboard Cite

Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene expression profiles of appressorium development provides insight into the molecular basis of pathogenicity and control of this fungal plant disease. A cDNA array representing 2927 unique genes based on a large EST (expressed sequence tag) database of M. grisea strain Y34 was constructed and used to profile the gene expression patterns at mycelium and appressorium maturation stages. Compared with mycelia, 55 up-regulated and 22 down-regulated genes were identified in mature appressoria. Among 77 genes, 16 genes showed no similarity to the genome sequences of M. grisea. A novel homologue of peptidyl-prolyl cis-trans isomerase was found to be expressed at low-level in mature appressoria of M. grisea. The results indicated that the genes such as pyruvate carboxylase, phospholipid metabolism-related protein and glyceraldehyde 3-phosphate dehydrogenase involved in gluconeogenesis, lipid metabolism and glycolysis, showed differential expression in mature appressoria. Furthermore, genes such as PTH11, beta subunit of G protein and SGT1 involved in cell signalling, were expressed differentially in mature appressoria. Northern blot analysis was used to confirm the cDNA array results.

show abstract

“…Two PISTILLATA (PI)-type class-B MADS-box genes, WPI1 and WPI2, and class D MADSbox genes, as well as WPPK1 (wheat pistillody-related protein kinase 1) were shown to be associated with the formation of pistil-like stamens in alloplastic wheat (Hama et al 2004;Saraike et al 2007). The distinct up-and downregulated expression changes of genes encoding MMK1 and MMK2 were observed at different day length/temperature regimes by Guo et al (2006), and suggested that the signal transduction pathway mediated by MAPK might be involved in the process regulating gene expression during fertility reversion. The wtms1 gene responsible for the sterility of the thermo-sensitive genic male-sterile wheat line BNY-S was mapped to chromosome 2B, and the transcript level of the candidate gene, TaAPT2, was decreased significantly in the young spikelets at an early stage of pollen development under low temperature stress (Xing et al 2003(Xing et al , 2005.…”

Section: Introductionmentioning

confidence: 98%

A ras GTPase-activating protein-binding protein, TaG3BP, associated with the modulation of male fertility in a thermo-sensitive cytoplasmic male sterile wheat line

Zhou

Gao

et al. 2011

Mol Genet Genomics

View full text Add to dashboard Cite

Hybrid wheat development may contribute to higher, more stable yield and could result in greater food security for much of the world's growing population. YS type thermo-sensitive cytoplasmic male sterile (TCMS) wheat lines were developed for use in hybrid wheat breeding in China. To investigate the molecular mechanism of modulation of male fertility in the YS-type TCMS wheat lines, a ras GTPase-activating protein-binding protein (TaG3BP) was examined. The deduced amino acid sequence encoded by TaG3BP was conserved in the sequenced genomes of Embryophyte. TaG3BP expression in the anthers of YS-type TCMS lines taken at the critical fertility reversion stage of pollen development from male fertile anthers was higher than that from male-sterile anthers, either by quantitative real-time PCR or by western blot analysis. Sequence analysis on the cDNA and genomic DNA of TaG3BP in three kinds of K-type CMS wheat lines and their maintainers indicated that there were no significant difference between the genes or in their 5' flanking sequences. The TaG3BP expression revealed by quantitative real-time RT-PCR was lower in the young spikes of these CMS lines than that of their maintainers. This indicates that TaG3BP expression is associated with the modulation, from male-sterile to fertile, of the TCMS wheat line, and TaG3BP might be a key factor in the pathway responsible for the fertility reversion.

show abstract

Gene expression profiling under different photoperiod/temperature conditions in a photoperiod-/thermo-sensitive genic male sterile line of rice (Oryza sativa L.)

Cited by 6 publications

References 15 publications

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea

A ras GTPase-activating protein-binding protein, TaG3BP, associated with the modulation of male fertility in a thermo-sensitive cytoplasmic male sterile wheat line

Contact Info

Product

Resources

About