Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets

Sarson, Aimie J.; Wang, Ying; Kang, Zhumei; Dowd, Scot E.; Lü, Yang; Yu, Hai; Han, Yanming; Zhou, Huaijun; Gong, Joshua

doi:10.1186/1471-2164-10-260

Cited by 31 publications

(19 citation statements)

References 60 publications

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“…Based on these results, the authors concluded that both cell-mediated and antibody-mediated immune responses were responsible for the host defense against C. perfringens infection. In a subsequent microarray study, Sarson et al (2009) confirmed that the expression of genes involved in inflammation, humoral immunity, antigen recognition, apoptosis, and immune-related metabolic processes were significantly altered after experimental infection of chickens with C. perfringens.…”

Section: Cytokine and Toll-like Receptor Expressionsupporting

confidence: 73%

Avian necrotic enteritis: Experimental models, host immunity, pathogenesis, risk factors, and vaccine development

et al. 2011

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The increasing trends of legislative restrictions and voluntary removal of antibiotic growth promoters worldwide has already affected, and will continue to affect, poultry production and animal health. Necrotic enteritis (NE) is being considered among the most important infectious diseases in the current poultry production system globally, with an estimated annual economic loss of more than $2 billion, largely attributable to medical treatments and impaired growth performance. Thus, there is an urgent need to develop rational, alternative, and integrated management strategies not only to control NE, but also to prevent it. In both humans and many warm-blooded animals and birds, NE is caused by Clostridium perfringens, a gram-positive, anaerobic, spore-forming bacterium. To accomplish these goals, better understanding of host- and environmentally related factors on the development of NE and potential vaccination strategies against C. perfringens infection will be necessary. Furthermore, a reliable and reproducible NE disease model is needed for characterization of C. perfringens pathogenesis and host protective immunity. This review summarizes recent developments in NE disease models, pathogenesis, host immunity, risk factors, and vaccine development for C. perfringens-associated NE in poultry.

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Section: Cytokine and Toll-like Receptor Expressionsupporting

confidence: 73%

Avian necrotic enteritis: Experimental models, host immunity, pathogenesis, risk factors, and vaccine development

et al. 2011

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“…Unlike a short oligonucleotide library, a long oligo nucleotide microarray can provide significantly improved sensitivity and can even detect one single transcript in one cell [18]. Previous studies have shown that the 44K Agilent chicken long oligonucleotide microarray can provide reliable results by detecting different chicken tissues [19]. Moreover, in order to improve the accuracy, three tissues from different individuals were pooled together for RNA extraction and hybridization in this study.…”

Section: Discussionmentioning

confidence: 99%

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl

Koltes¹,

Deng²,

Li³

et al. 2014

Front. Agr. Sci. Eng.

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Melanoblasts originating from neural crest cells can migrate through the mesenchyme of the developed embryo and give rise to melanocytes. Unlike the melanocytes that are confined to the integument in other vertebrates, melanocytes in Silky Fowl can reach the ventral regions of the embryos owing to differences in gene expression in the process of melanoblasts migration. In this study, we used microarray profiling to identify differences in gene expression between White Leghorn and Silky Fowl. Differential expression of 2517 microarray probes (P < 0.01, Fold Change > 2) was observed in Silky Fowl compared to White Leghorn. After filtration by cluster analysis, functional annotation and pathway analysis, eight differentially expressed genes were identified to be closely related to the development of melanocytes. Moreover, differences in expression of immune genes were also detected between Silky Fowl and White Leghorn. The differentially expressed genes associated with melanocyte development were verified by q-PCR, and results were highly consistent with the microarray data. The genes with significantly altered expression involved in melanoblast migration and development suggested that different microenvironments resulted in the abnormal melanoblast migration in Silky Fowl, although there were no big differences in melanoblast development between these two breeds. The candidate genes discovered in this study are beneficial to understand the molecular mechanism of hyperpigmentation in Silky Fowl.

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“…The chickens used for the present study were the same birds used for our previous reports of alpha-toxin gene expression in the chicken intestine (35) and the host response to C. perfringens infection as evaluated by both a low-density chicken immune cDNA microarray and a 44,000-slide-format whole-chicken-genome Agilent microarray (34,42). The chickens were cared for under the 1993 guidelines of the Canadian Council on Animal Care.…”

Section: Bacteriummentioning

confidence: 99%

Expression Profiles of Genes in Toll-Like Receptor-Mediated Signaling of Broilers Infected with Clostridium perfringens

Lü

Sarson

Gong

et al. 2009

Clin Vaccine Immunol

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Toll-like receptors (TLRs) participate in detecting microbial pattern molecules for activation of the host immune response. We investigated possible roles of TLRs in the chicken response to Clostridium perfringens infection by examining the expression of TLR genes and other genes involved in TLR-mediated signaling within the spleens and ilea of C. perfringens-challenged broilers. Upregulation of a tumor necrosis factor alphainducing factor homolog in challenged chickens compared to naïve chickens was observed, regardless of the incidence of necrotic enteritis. In addition, the members of the TLR2 subfamily were found to be most strongly involved in the host response to C. perfringens challenge, although the expression of TLR4 and TLR7 was also upregulated in spleen tissues. While the combination of TLR1.2, TLR2.1, and TLR15 appeared to play a major role in the splenic response, the expression of TLR2.2 and TLR1.1 was positively correlated to the expression of adaptor molecules MyD88, TRAF6, TRIF, and receptor interacting protein 1 in the ileal tissues, demonstrating a dynamic spatial and temporal innate host response to C. perfringens.

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Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets

Cited by 31 publications

References 60 publications

Avian necrotic enteritis: Experimental models, host immunity, pathogenesis, risk factors, and vaccine development

Avian necrotic enteritis: Experimental models, host immunity, pathogenesis, risk factors, and vaccine development

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl

Expression Profiles of Genes in Toll-Like Receptor-Mediated Signaling of Broilers Infected with Clostridium perfringens

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