Gene expression signatures differentiate adenocarcinoma of lung and breast origin in effusions

Davidson, Ben; Stavnes, Helene Tuft; Risberg, Bjørn; Nesland, Jahn M.; Wohlschlaeger, Jeremias; Yang, Yanqin; Shih, Ie Ming; Wang, Tian Li

doi:10.1016/j.humpath.2011.06.015

Cited by 31 publications

(43 citation statements)

References 51 publications

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“…This material has been extensively used for both gene expression array analyses of different cancers affecting the serosal cavities [73][74][75] and validation studies of the array data using qPCR [76][77][78][79][80][81][82].…”

Section: Other Ancillary Testingmentioning

confidence: 99%

Pre-analytical issues in effusion cytology

Michael

Davidson²

2016

Pleura and Peritoneum

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Effusions or body cavity fluids are amongst the most commonly submitted samples to the cytology laboratory. Knowledge of proper collection, storage, preservation and processing techniques is essential to ensure proper handling and successful analysis of the sample. This article describes how the effusions should be collected and proper conditions for submission. The different processing techniques to extract the cellular material and prepare slides satisfactory for microscopic evaluation are described such as direct smears, cytospins, liquid based preparations and cell blocks. The article further elaborates on handling the specimens for additional ancillary testing such as immunostaining and molecular tests, including predictive ones, as well as future research approaches.

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Section: Other Ancillary Testingmentioning

confidence: 99%

Pre-analytical issues in effusion cytology

Michael

Davidson²

2016

Pleura and Peritoneum

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“…In the following years other aGPCRs such as GPR56 were identified to be cancer related [17]. Non-biased screens such as mRNA microarrays, genome-wide association (GWA) studies, or proteome analyses confirmed previously identified cancer-related changes in aGPCRs [18], showed alterations in new tumor entities [19], or identified new aGPCRs involved in cancer [20]. Recently, a whole-genome sequencing (WGS) approach in 441 human breast, lung, ovarian, and prostate cancers identified the aGPCRs ADGRB3 (BAI3) and ADGRL3 (LPHN3) among 77 significantly mutated genes [21].…”

Section: Identification Of Adhesion Gpcrs In Cancermentioning

confidence: 90%

“…GPR116, highly enriched in fetal and adult lung [144][145][146], is part of a gene expression signature that differentiates adenocarcinoma of lung and breast origin in effusions [20]. Knockdown of GPR116 suppresses migration and invasion, whereas ectopic expression enhances invasion of the breast cancer cell line MDA-MB-231 [65].…”

Section: Adgrfmentioning

confidence: 99%

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Adhesion-GPCRs in Tumorigenesis

Aust

2010

Advances in Experimental Medicine and Biology

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Alterations in the homeostasis of several adhesion GPCRs (aGPCRs) have been observed in cancer. The main cellular functions regulated by aGPCRs are cell adhesion, migration, polarity, and guidance, which are all highly relevant to tumor cell biology. Expression of aGPCRs can be induced, increased, decreased, or silenced in the tumor or in stromal cells of the tumor microenvironment, including fibroblasts and endothelial and/or immune cells. For example, ADGRE5 (CD97) and ADGRG1 (GPR56) show increased expression in many cancers, and initial functional studies suggest that both are relevant for tumor cell migration and invasion. aGPCRs can also impact the regulation of angiogenesis by releasing soluble fragments following the cleavage of their extracellular domain (ECD) at the conserved GPCR-proteolytic site (GPS) or other more distal cleavage sites as typical for the ADGRB (BAI) family. Interrogation of in silico cancer databases suggests alterations in other aGPCR members and provides the impetus for further exploration of their potential role in cancer. Integration of knowledge on the expression, regulation, and function of aGPCRs in tumorigenesis is currently spurring the first preclinical studies to examine the potential of aGPCR or the related pathways as therapeutic targets.

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“…9,10 PITX1 is required for pituitary gland and hind limb development. 11 It is also known to interact functionally with other transcription factors such as SF-1 and basic helix-loop-helix transcription factors.…”

Section: Introductionmentioning

confidence: 99%