Gene expression via direct injection of plasmid vector into thymus

Immune response and inhibition of bacterial growth by electrotransfer of plasmid DNA containing the antimicrobial peptide, epinecidin-1, into zebrafish muscle

“…The first in vivo report of gene transfer with naked DNA following injection of messenger (m)RNA or plasmid DNA into skeletal muscle was demonstrated with transfection in myofibers [2]. Following the success of that experiment, many studies using the direct injection of DNA for foreign gene expression in other tissues such as the heart, thyroid, skin, and liver were published [3][4][5]. Those results were concerned with the efficiency of gene transfer into muscle which showed relatively low expression in non-human primates [6].…”