Gene flow across host‐associated populations of the rice stem borer <i>Chilo suppressalis</i> Walker (Lepidoptera: Crambidae): implications for <i>Bt</i> resistance management in rice

Wang, Yongmo; Huang, Chen; Hu, Bing; Liu, Yue; Walter, Gimme H.; Hereward, James

doi:10.1002/ps.5567

Cited by 7 publications

(3 citation statements)

References 60 publications

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“…Previous studies have shown that comparisons of differences in agronomic traits ( Xia et al, 2011 ; Wang et al, 2012a ; Li et al, 2013 ; Jiang et al, 2016 ), nutritional composition ( Wang et al, 2012b ; Jian et al, 2014 ; Jiang et al, 2018 ; Takeda et al, 2019 ), environmental adaptability ( Jiang et al, 2018 ; Wang et al, 2019 ; Park et al, 2019 ), gene transcription, protein expression, and metabolite levels ( Liu et al, 2012 ; Liu Y. et al, 2015 ; Fu et al, 2019 ; Peng et al, 2019 ) among transgenic crops and their parents are important means and strategies for confirming and excluding the unintended effects of transgenic crops, in addition to facilitating any safety evaluations. However, the molecular mechanisms of underlying the unintended effects observed in transgenic crops are unclear.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Cry1Ab/c Protein Recruits Different Endogenous Proteins for Its Function in Plant Growth and Development

Liu

2020

Front. Bioeng. Biotechnol.

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Current risk assessments of transgenic crops do not take into consideration whether exogenous proteins interact with endogenous proteins and thereby induce unintended effects in the crops. Therefore, the unintended effects through protein interactions in insect-resistant transgenic rice merit investigation. Here, a yeast two-hybrid assay was used to evaluate interactions between Bacillus thuringiensis (Bt) protein-derived Cry1Ab/c insect resistance rice Huahui-1 and the endogenous proteins of its parental rice Minghui-63. The authenticity of the strongest interactions of Cry1Ab/c and 14 endogenous proteins involved in photosynthesis and stress resistance, which may be primarily responsible for the significant phenotypic differences between transgenic Huahui-1 and parental Minghui-63, were then analyzed and validated by subcellular colocalization, bimolecular fluorescence complementation and co-immunoprecipitation. As the exogenous full-length Cry1Ab/c protein was found to have self-activating activity, we cleaved it-into three segments based on its three domains, and these were screened for interaction with host proteins using the yeast two-hybrid assay. Sixty endogenous proteins related to the regulation of photosynthesis, stress tolerance, and substance metabolism were found to interact with the Cry1Ab/c protein. The results of bimolecular fluorescence complementation and co-immunoprecipitation verified the interactions between the full-length Cry1Ab/c protein and 12 endogenous proteins involved in photosynthesis 23KD, G, PSBP, Rubisco, Trx, THF1 and stress resistance CAMTAs, DAHP, E3s, HKMTs, KIN13A, FREE1. We used a combination of yeast two-hybrid, bimolecular fluorescence complementation, and co-immunoprecipitation to identify Cry1Ab/c interacting with rice proteins that seem to be associated with the observed unintended effects on photosynthesis and stress resistance between Huahui-1 and Minghui-63 rice plants, and analyze the possible interaction mechanisms by comparing differences in cell localization and interaction sites between these interactions. The results herein provide a molecular analytical system to qualify and

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Section: Discussionmentioning

confidence: 99%

Exogenous Cry1Ab/c Protein Recruits Different Endogenous Proteins for Its Function in Plant Growth and Development

Liu

2020

Front. Bioeng. Biotechnol.

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“…Moreover, molecular data can be used to trace pest dispersal routes and to infer whether gene flow occurs between populations (Franklin et al, 2011; Li et al, 2015; Otim et al, 2018). This has practical significance, e.g., regarding the spread of mutations affecting fitness and the effectiveness of refuge crops to delay evolution of Bacillus thuringiensis Berliner (Bt) resistance (Miller & Sappington, 2017; Wang et al, 2020). In this context, mitochondrial DNA (mtDNA) has proven useful for species identification and for population genetic studies, because of its maternal inheritance, relatively high mutation rates, and low recombination rates (Avise et al, 1987; Galtier et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas

Decker‐Franco,

San Blas,

Balbi

et al. 2023

Entomologia Exp Applicata

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Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae, Plusiinae) is a defoliator of soybean (Glycine max L., Fabaceae), sunflower (Helianthus annuus L., Asteraceae), alfalfa (Medicago sativa L., Fabaceae), and horticultural crops in temperate southern South America; however, there is little genetic information available about this pest insect. Recently, concerns about this insect have increased because of its suspected resistance to Bt soybean and its spread to tropical regions. To better understand pest dynamics, and therefore implement suitable management strategies, a thorough knowledge of the genetic variability and spatial structuring of populations is required. In this paper, R. nu larvae were sampled from various crops and locations in central Argentina (where the pest is endemic) to assess diversity and population structure through mtDNA sequence analysis. In addition to the analysis of the standard cytochrome c oxidase subunit I (COI) ‘barcode’ fragment, a new set of primers was developed to explore genetic variation in the cytochrome b (Cytb) gene. COI and Cytb concatenated sequences revealed an overall high haplotype diversity (mean ± SD = 0.798 ± 0.037) and low nucleotide diversity (0.00137 ± 0.00013). The haplotype network showed a total of 19 haplotypes, separated by at most five mutational steps. A main central haplotype and four additional frequent haplotypes (accounting for 80% of the specimens examined) were distributed across populations with no obvious pattern related to host plant or geographic origin. Analysis of molecular variance further supported a weak population structure, where genetic variation within populations was the main source of total variation (ca. 96%). Pairwise comparisons showed only minor genetic differentiation among some of the populations studied. Implications of these findings in the context of R. nu control are discussed.

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“…Species complexes, composed of a group of closely related species, often lack morphological characters for species identification, which can lead to shortcomings in our understanding of a specific insect's biology and impede the development of new strategies to manage pests [4][5][6][7][8][9]. Misidentification of pest species can reduce the effectiveness of management programs targeting plant and human diseases vectors [5,8], slow the advancement of biological control efforts [6] and potentially increase grower dependency on the prophylactic use of insecticides.…”

Section: Introductionmentioning

confidence: 99%

Characterizing Billbug (Sphenophorus spp.) Seasonal Biology Using DNA Barcodes and a Simple Morphometric Analysis

Rodriguez-Soto

Richmond

Xiong

et al. 2021

Insects

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Billbugs (Sphenophorus spp.) are a complex of grass-feeding weevil species that reduce the aesthetic and functional qualities of turfgrass. Effective billbug monitoring and management programs rely on a clear understanding of their seasonal biology. However, our limited understanding of regional variation in the species compositions and seasonal biology of billbugs, stemming primarily from our inability to identify the damaging larval stage to species level, has hindered efforts to articulate efficient IPM strategies to growers. We used a combination of DNA barcoding methods and morphometric measures to begin filling critical gaps in our understanding of the seasonal biology of the billbug species complex across a broad geographic range. First, we developed a DNA barcoding reference library using cytochrome oxidase subunit 1 (COI) sequences from morphologically identified adult billbugs collected across Indiana, Missouri, Utah and Arizona. Next, we used our reference library for comparison and identification of unknown larval specimens collected across the growing season in Utah and Indiana. Finally, we combined our DNA barcoding approach with larval head capsule diameter, a proxy for developmental instar, to develop larval phenology charts. Adult COI sequences varied among billbug species, but variation was not influenced by geography, indicating that this locus alone was useful for resolving larval species identity. Overlaid with head capsule diameter data from specimens collected across the growing season, a better visualization of billbug species composition and seasonal biology emerged. This approach will provide researchers with the tools necessary to fill critical gaps in our understanding of billbug biology and facilitate the development of turfgrass pest management programs.

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Gene flow across host‐associated populations of the rice stem borer Chilo suppressalis Walker (Lepidoptera: Crambidae): implications for Bt resistance management in rice

Cited by 7 publications

References 60 publications

Exogenous Cry1Ab/c Protein Recruits Different Endogenous Proteins for Its Function in Plant Growth and Development

Exogenous Cry1Ab/c Protein Recruits Different Endogenous Proteins for Its Function in Plant Growth and Development

Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas

Characterizing Billbug (Sphenophorus spp.) Seasonal Biology Using DNA Barcodes and a Simple Morphometric Analysis

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