Gene frequencies of platelet-specific antigens in Croatian population

Abstract

The human platelet antigens (HPA) are genetically defined polymorphisms expressed on platelet membrane glycoproteins. As platelet antigens are very important in several clinical situations and in population genetics, we used the polymerase chain reaction with sequence-specific primers (PCR-SSP) to investigate HPA-1, -2, -3 and -5 allele frequencies in the Croatian population. The HPA frequencies obtained in 219 Croatians were: 1a-0.854, 1b-0.146, 2a-0.890, 2b-0.110, 3a-0.575, 3b-0.425, 5a-0.895 and 5b-0.105. These data are similar to the frequencies reported in most European studies with some significant differences in HPA-2 when compared with the Dutch and German population, in HPA-3 when compared with the Swiss population and in HPA-5 when compared with the Finnish population. The three most common condensed HPA genotypes in the Croatian population were: HPA-1a/a, -2a/a, -3a/b, -5-a/a (0.283), HPA-1a/a, -2a/a, -3a/a, -5-a/a (0.137) and HPA-1a/b, -2a/a, -3a/b, -5-a/a (0.087). Data obtained in this study can be used for better understanding and treatment of immune-mediated platelet disorders in our population.

Human platelet antigen genotyping of platelet donors in southern Brazil

“…No significant differences in allele frequencies were observed between our Caucasian group and the populations of Argentina (De La Vega Elena et al ., ; HPA‐1 to 5 and 15), Italy (EBI, ; HPA‐1 to 5), Germany (EBI, ; HPA‐1 to 3 and 5), Croatia (Pavic et al ., ; HPA‐1 to 3 and 5), the United Kingdom (Jones et al ., ; HPA‐1 to 5), Slovenia (Rozman et al ., ; HPA‐1 to 5), France (Merieux et al ., ; HPA‐1 to 5), Denmark (Steffensen et al ., ; HPA‐1 to 5), Austria (Holensteiner et al ., ; HPA‐1 to 3 and 5), Poland (Drzewek et al ., ; HPA‐1 to 5) or Spain (Muniz‐Diaz et al ., ; HPA‐2 to 5 and 15). For HPA‐1, we found a significant difference between our sample and the Spanish population (HPA‐1a = 0.867 and 1b = 0.133 vs. HPA‐1a = 0.810 and 1b = 0.190, respectively, P < 0.05).…”

“…To assess the ability of HPA alleles to describe intercontinental genetic divergence, we conducted an amova , which quantifies separation between populations. Towards this end, we divided the assessed populations into two groups: one comprising the populations of Asia and Oceania, as well as native ethnic groups – Toba Amerindians (De La Vega Elena et al ., ), Parakanã Indians (Castro et al ., ), Native Brazilians, Maori people (Edinur et al ., ), Korea (Pavic et al ., ), Malaysia (Tan et al ., ), Indonesia (Asmarinah et al ., ), China (Nie et al ., ) and Polynesia (Edinur et al ., ) – and one comprising the populations of European and African origin: Poland (Drzewek et al ., ), Denmark (Steffensen et al ., ), Italy (EBI, ), Spain (Muniz‐Diaz et al ., ), the United Kingdom (Jones et al ., ), Slovenia (Rozman et al ., ), Argentina (De La Vega Elena et al ., ), the Congo (Halle et al ., ) and Egypt (Salem et al ., ). Argentina was included in this group because the makeup of its population is strongly European.…”

Genotyping of Eight Human Platelet Antigen Systems in Serbian Blood Donors: Foundation for Platelet Apheresis Registry

“…HPA-1 bb homozygotes are at the biggest risk of immunization, because they may form anti-HPA-1a antibodies which are, as previously mentioned, responsible for 80% of FNAIT cases [3, 9]. The frequency of the HPA-1 bb genotype is indicated to be 1.4% in the Serbian population, which correlates with its frequency in other populations of European origin [10, 16, 20, 21, 23].…”

“…Published data of other nations of Southeastern Europe reveal results similar to those of the Serbian population. HPA allele frequencies in populations of Slovenia (HPA-1 to HPA-5) [16], North Macedonia (HPA-1 to HPA-3 and HPA-5) [20], and Croatia (HPA-1 to HPA-3, HPA-5, and HPA-15) [21, 22], as shown in Table 3, all showed no significant differences in comparison with our results ( p > 0.05 for all populations and HPA systems that were tested in these studies). Similarly, statistically significant differences were not encountered between the Serbian population and data from non-Finnish European populations from the gnomAD database [23], or other European populations [10].…”

The human platelet alloantigen profile in blood donors from Amazonas, Brazil

“…The genotype and allele frequencies were estimated by direct counting, and the results were compared with the values published for blood donors and/or healthy individuals worldwide (http://www.ebi.ac.uk/ipd/hpa/freqs_1.html). A 2 test or Fisher's exact test was performed to individually compare the allele frequencies of each HPA polymorphic system between the Amazonas blood donors and the data reported for 38 population groups worldwide (Muniz-Diaz et al, 1993;Santoso et al, 1993;Holensteiner et al, 1995;Kekomaki et al, 1995;Kim et al, 1995;Tanaka et al, 1995;Steffensen et al, 1996;Carlsson et al, 1997;Merieux et al, 1997;Drzewek et al, 1998;Seo et al, 1998;Castro et al, 1999;Korinkova et al, 1999;Mojaat et al, 1999;Rozman et al, 1999;Sellers et al, 1999;Mazzucco et al, 2000;Nogues et al, 2000;Bennett et al, 2002;Ferrer et al, 2002;Boehlen et al, 2003;Jones et al, 2003;Randen et al, 2003;Shih et al, 2003;Halle et al, 2004Halle et al, , 2005Kulkarni et al, 2005;Feng et al, 2006;Pavkovic et al, 2006;De La Vega Elena et al, 2008;Bhatti et al, 2010;Pavic et al, 2010;Tan et al, 2012;Conti et al, 2014). A hierarchical classification of all analysed populations was carried out based on a allele frequencies from five HPA polymorphic systems using Cluster 3·0 and Java Tree View software packages (Eisen Lab, University of California, Berkeley).…”