1999
DOI: 10.1097/00002371-199903000-00005
|View full text |Cite
|
Sign up to set email alerts
|

Gene Gun-Mediated IL-12 Gene Therapy Induces Antitumor Effects in the Absence of Toxicity: A Direct Comparison With Systemic IL-12 Protein Therapy

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3

Citation Types

3
46
0

Year Published

2001
2001
2004
2004

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 73 publications
(49 citation statements)
references
References 0 publications
3
46
0
Order By: Relevance
“…8,9 This antitumor effect is -mediated primarily by CD8 + T cells, which leads to the development of tumorspecific immunological memory. 10 However, from the clinical point of view, this approach is practical only for skin cancers such as melanoma or cutaneous T-cell lymphoma, 8 but not for other types of cancer.…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…8,9 This antitumor effect is -mediated primarily by CD8 + T cells, which leads to the development of tumorspecific immunological memory. 10 However, from the clinical point of view, this approach is practical only for skin cancers such as melanoma or cutaneous T-cell lymphoma, 8 but not for other types of cancer.…”
Section: Discussionmentioning
confidence: 99%
“…6,7 IL-12 gene therapy using gene gun technology has been shown to exhibit a remarkable antitumor effect without toxicity in murine models. 8,9 Rakhmilevich et al reported that transfer of the IL -12 gene into the skin overlying murine tumors using a gene gun effectively eradicates established primary intradermal tumors and their spontaneous metastasis. 10 These antitumor effects are mediated primarily by CD8 + T cells, which lead to the development of tumorspecific immunological memory.…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…This would circumvent several problems such as systemic toxicity, rapid degradation and the short half-life associated with systemic delivery of various recombinant cytokines. 16 IL-12 is an immune regulatory cytokine, which plays multiple roles in altering the host-tumor relationship, such as: (1) enhancement of the cellular immune mechanism to favor activation and proliferation of CD4 + helper T cells and CD8 + cytotoxic T cells leading to potent Th1 type immune response; (2) recruitment of cytotoxic NK and NKT cells to directly participate in early stages of tumor killing; and (3) up-regulation of endogenous IFN-␥ and TNF-␣, as well as NO to suppress tumor progression, metastasis and angiogenesis. 17 In the present study, we investigated the role of IL-12 gene delivery with a broader perspective, looking into its role in simultaneous induction of various anti-tumoral cytokines, cytotoxic NO, as well as its role in recruitment of various immune cells, which form a part of innate and adaptive immunity, resulting in anti-tumor activity.…”
Section: Discussionmentioning
confidence: 99%
“…20 Low systemic levels of IL-12 have been shown to induce IFN-␥ production and, through feedback control mechanisms, IL-12 would activate its own up-regulation through macrophages, dendritic cells and other stimulatory factors, such as NO induced by IFN-␥ as a part of adaptive immune response. 16,19 We, therefore, determined the induced levels of IFN-␥ and TNF-␣ after intratumoral injection of PAGA/pmIL-12 complexes. The production of both induced cytokines and mIL-12 was significantly up-regulated compared with control mice injected with 5% glucose (Figures 1-3).…”
Section: Discussionmentioning
confidence: 99%