Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan Equine Encephalitis virus

Bennett, Alice M.; Phillpotts, R. J.; Perkins, Stephanie D.; Jacobs, Sydney; Williamson, E. Diane

doi:10.1016/s0264-410x(99)00317-5

Cited by 62 publications

(33 citation statements)

References 35 publications

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“…Langerhans cells) of the viable epidermis [19,20]. Since the earliest DNA vaccine studies, direct comparisons of PMED to needle-based approaches for DNA vaccine delivery in mice [21][22][23][24][25][26][27] and monkeys [28][29][30][31] have shown that PMED can induce higher antibody and/or CD8 + T cell responses with substantially lower doses (100-1000-fold) of DNA. The eVectiveness of this system is likely related to the use of a delivery technology that deposits DNA directly into cells [18,20] as well as the immune competence of the epidermis as a delivery site [32,33].…”

Section: Particle-mediated Epidermal Delivery Of Dna Vaccinesmentioning

confidence: 99%

Preclinical and clinical progress of particle-mediated DNA vaccines for infectious diseases

Fuller

Loudon

Schmaljohn

2006

Methods

138

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This review provides an overview of studies employing particle-mediated epidermal delivery (PMED) or the gene gun to administer DNA vaccines for infectious diseases in preclinical studies employing large animal models and in human clinical trials. It reviews the immunogenicity and protective eYcacy of PMED DNA vaccines in nonhuman primates and swine and studies that have directly compared the eVectiveness of PMED in these large animal models to existing licensed vaccines and intramuscular or intradermal delivery of DNA vaccines with a needle. Various clinical trials employing PMED have been completed and an overview of the immunogenicity, safety, and tolerability of this approach in humans is described. Finally, eVorts currently in progress for commercial development of particle-mediated DNA vaccines are discussed.

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Section: Particle-mediated Epidermal Delivery Of Dna Vaccinesmentioning

confidence: 99%

Preclinical and clinical progress of particle-mediated DNA vaccines for infectious diseases

Fuller

Loudon

Schmaljohn

2006

Methods

138

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“…For the penetration stage, the effect of the skin is the major resistance to prevent the microparticle delivery. Micro-particle delivery requires breaching of the SC and piercing into the epidermis layer (Trainer & Alexander, 1997;Bennett et al, 1999;Quinlan et al, 2001;Liu, 2006;Yager et al, 2013). The impact velocity, particle size and density, target density and yield stress are the major variables affecting the penetration depth.…”

Section: Modeling Micro-particle Delivery In Skinmentioning

confidence: 99%

“…A gene gun is considered to be a unique concept which was first used to deliver genetic materials into plant cells (Klein et al, , 1992Sanford et al, 1993;Svarovsky, 2008;Huang & Chen, 2011;O'Brien & Lummis, 2011;Manjila et al, 2013). The technique has been used to transfer DNAcoated micro-particles to achieve gene transfection into many types of cells and organs (Bennett et al, 1999;Meacham et al, 2013), for example, mammals (Williams et al, 1991;Kuriyama et al, 2000;Sakai et al, 2000;Da'dara et al, 2002;Ettinger et al, 2012;Cao et al, 2013), plants (Klein et al, 1992;Zuraida et al, 2010;Kuriakose et al, 2012), artificially cultured cells (O'Brien & Lummis, 2006, 2011, fungi (Armaleo et al, 1990;Gu et al, 2011) and bacteria (Smith et al, 1992;Nagata & Koide, 2010). A number of commercial gene guns have been manufactured and used for in vivo gene transfection in plants, living animals, cultured cells, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…This also means that while gene guns have been reported to be successful in many instances, they may have some disadvantages. Generally, for most gene gun systems, the maximum penetration of the micro-particle can breach the stratum corneum and end inside the epidermis layer of the tissue (Bennett et al, 1999;Quinlan et al, 2001;Liu, 2006;Yager et al, 2013). The epidermis layer is normally considered to be a target site for gene delivery due to its accessibility (Trainer & Alexander, 1997;Quinlan et al, 2001;Liu, 2006;.…”

Section: Introductionmentioning

confidence: 99%

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Potential of microneedle-assisted micro-particle delivery by gene guns: a review

2013

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“…Generally, the epidermis layer of the skin is considered as the main target of these microparticles (Trainer & Alexander, 1997;Bennett et al, 1999;Quinlan et al, 2001;Liu, 2006;. However, cell and tissue damages are particular problems for the use of gene guns (Yoshida et al, 1997;Sato et al, 2000;Thomas et al, 2001;Uchida et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Microneedle-assisted microparticle delivery by gene guns: experiments and modeling on the effects of particle characteristics

2014

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Microneedles (MNs) have been shown to enhance the penetration depths of microparticles delivered by gene gun. This study aims to investigate the penetration of model microparticle materials, namely, tungsten (51 mm diameter) and stainless steel (18 and 30 mm diameters) into a skin mimicking agarose gel to determine the effects of particle characteristics (mainly particle size). A number of experiments have been processed to analyze the passage percentage and the penetration depth of these microparticles in relation to the operating pressures and MN lengths. A comparison between the stainless steel and tungsten microparticles has been discussed, e.g. passage percentage, penetration depth. The passage percentage of tungsten microparticles is found to be less than the stainless steel. It is worth mentioning that the tungsten microparticles present unfavourable results which show that they cannot penetrate into the skin mimicking agarose gel without the help of MN due to insufficient momentum due to the smaller particle size. This condition does not occur for stainless steel microparticles. In order to further understand the penetration of the microparticles, a mathematical model has been built based on the experimental set up. The penetration depth of the microparticles is analyzed in relation to the size, operating pressure and MN length for conditions that cannot be obtained in the experiments. In addition, the penetration depth difference between stainless steel and tungsten microparticles is studied using the developed model to further understand the effect of an increased particle density and size on the penetration depth.

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Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan Equine Encephalitis virus

Cited by 62 publications

References 35 publications

Preclinical and clinical progress of particle-mediated DNA vaccines for infectious diseases

Preclinical and clinical progress of particle-mediated DNA vaccines for infectious diseases

Potential of microneedle-assisted micro-particle delivery by gene guns: a review

Microneedle-assisted microparticle delivery by gene guns: experiments and modeling on the effects of particle characteristics

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