Gene Insertion into the Chicken Germ Line by Retroviruses

Salter, Donald W.; Smith, E. A.; Hughes, Stephen H.; Wright, Stephen E.; Fadly, A. M.; Witter, R. L.; Crittenden, L. B.

doi:10.3382/ps.0651445

Cited by 127 publications

(68 citation statements)

References 42 publications

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“…A number of approaches have been pursued with varying degrees of success: manipulation of oocytes/spermatozoa or newly laid eggs; production of chimeras using primordial germ cells or chick ES cell equivalents; and gene transfer using viral vectors (Mozdziak and Petitte, 2004;Petitte et al, 2004;Sang, 2004). Oncogenic viruses have shown promise (Ishii et al, 2004), including avian leucosis virus (ALV) and reticuloendotheliosis virus (REV) (Salter et al, 1986;Salter et al, 1987;Bosselman et al, 1989a;Bosselman et al, 1989b), and Moloney murine leukemia virus (MoMLV) (Mizuarai et al, 2001). A newly reported MoMLVtransduced chick, utilizing the vesicular stomatitis virus G glycoprotein (VSV-G) vector pseudotyping system, expresses GFP under control of the rous sarcoma virus (RSV) promoter (Kwon et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Ubiquitous GFP expression in transgenic chickens using a lentiviral vector

Chapman

Lawson

MacArthur³

et al. 2005

Development

124

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We report the first ubiquitous green fluorescent protein expression in chicks using a lentiviral vector approach, with eGFP under the control of the phosphoglycerol kinase promoter. Several demonstrations of germline transmission in chicks have been reported previously, using markers that produce tissue-specific, but not ubiquitous, expression. Using embryos sired by a heterozygous male, we demonstrate germline transmission in the embryonic tissue that expresses eGFP uniformly, and that can be used in tissue transplants and processed by in situ hybridization and immunocytochemistry. Transgenic tissue is identifiable by both fluorescence microscopy and immunolabeling, resulting in a permanent marker identifying transgenic cells following processing of the tissue. Stable integration of the transgene has allowed breeding of homozygous males and females that will be used to produce transgenic embryos in 100% of eggs laid upon reaching sexual maturity. These results demonstrate that a transgenic approach in the chick model system is viable and useful even though a relatively long generation time is required. The transgenic chick model will benefit studies on embryonic development, as well as providing the pharmaceutical industry with an economical bioreactor.

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Section: Introductionmentioning

confidence: 99%

Ubiquitous GFP expression in transgenic chickens using a lentiviral vector

Chapman

Lawson

MacArthur³

et al. 2005

Development

124

View full text Add to dashboard Cite

show abstract

“…by Salter et al (1986Salter et al ( , 1987 who used replication competent reticuloendotheliosis virus (REV) and avian leukosis virus. The retroviral vectors were injected near the blastoderm resulting in 25% of the G0 males being mosaic for the transgene, and they transmitted the provirus in the germline at a rate of 1 to 11%.…”

Section: Retroviral Historical Perspectivementioning

confidence: 99%

Status of transgenic chicken models for developmental biology

Mozdziak

Petitte

2004

Developmental Dynamics

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The chick embryo is a classic model that has been used to gain insight into developmental processes and cell fate within the embryo for over a century. For the most part, investigators have implanted quail cells into a chicken embryo. A more powerful tool for developmental biology research than the quail:chick chimera system would be to have lines of transgenic chickens expressing reporter genes that are readily available to the research community. However, avian transgenic technology has been fraught with technical difficulties, and transgenic chickens expressing reporter genes have only recently been developed. The goal of this review is to report the technologies that have been used to generate transgenic chickens and to discuss the challenges in generating avian transgenics for developmental biology research. Developmental Dynamics 229:414 -421, 2004.

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“…Wei et al, 1981;Doehmer et al, 1982;Joyner et al, 1983;Yu et al, 1986;Kwok et al, 1986). Similar retroviral vector systems based on either avian leukosis virus or reticuloendotheliosis virus have been, or are being, developed, though so far their use has been experimental (Shuman and Shoffner, 1986;Hughes et al, 1986;Salter et al, 1986). The use of retroviral vectors places some constraints on the size and nature of the genetic material which can be transferred.…”

Section: Methods For Gene Transfermentioning

confidence: 99%