Gene introgression into Coffea arabica by way of triploid hybrids (C. arabica × C. canephora)

Herrera, Juan-Carlos; Combes, Marie‐Christine; Cortina, Hernando; Alvarado, Gabriel Alvarado; Lebailly, Philippe

doi:10.1038/sj.hdy.6800171

Cited by 28 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, genetic diversity of C. arabica is considered low, due likely to genetic bottlenecks caused by propagation of few individuals from selected cultivars for commercial purposes (Cubry et al, 2008;Lashermes et al, 2011). Thus, besides its economic and agricultural relevance, Coffea species and C. arabica cultivars can shed light on the domestication and evolution of this genus through analysis of genome sequence and nuclear genome size, among other approaches (Barre et al, 1996;Herrera et al, 2002). Genome size is incongruent with the ploidy level and number of basic chromosome sets of an organism (Huang et al, 2013;Tatum et al, 2006).…”

mentioning

confidence: 99%

Analysis of Genome Size of Sixteen Coffea arabica Cultivars Using Flow Cytometry

Ortega-Ortega¹,

Ramírez-Ortega²,

Ruíz-Medrano³

et al. 2019

horts

View full text Add to dashboard Cite

Coffee is an important crop worldwide, grown on about 10 million hectares in tropical regions including Latin America, Africa, and Asia. The genus Coffea includes more than 100 species; most are diploid, except for C. arabica, which is allotetraploid and autogamous. The genetic diversity of commercial coffee is low, likely due to it being selfpollinating, in addition, the widespread propagation of few selected cultivars, such as Caturra, Bourbon, and Typica. One approach is the analysis of genome size in these cultivars as a proxy to study its genetic variability. In the present work, genome size of 16 cultivars was assessed through high-resolution flow cytometry (FCM). Nuclear DNA was analyzed using a modified procedure that uses propidium iodide (PI) and 4#,6#-diamino-2-phenylindole dihydrochloride hydrate (DAPI) staining. The C. arabica cultivars investigated possessed a nuclear DNA content ranging from 2.56 ± 0.016 pg for Typica, to 3.16 ± 0.033 pg for ICATU, which had the largest genome size. All cultivars measured using both fluorochromes had greater estimates with DAPI than PI. The proportion of the genome composed of guanosine and cytosine (GC%) among the cultivars evaluated in this study ranged from 37.03% to 39.22%. There are few studies of genome size by FCM of distinct important C. arabica cultivars, e.g., hybrids and artificial crosses. Thus, this work could be valuable for coffee breeding programs. The data presented here are intended to expand the genomic understanding of C. arabica and could link nuclear DNA content with evolutionary relationships such as diversification, hybridization and polyploidy. Materials and Methods Plant materialLeaves were collected from 16 healthy C. arabica cultivars in consideration of its

show abstract

mentioning

confidence: 99%

Analysis of Genome Size of Sixteen Coffea arabica Cultivars Using Flow Cytometry

Ortega-Ortega¹,

Ramírez-Ortega²,

Ruíz-Medrano³

et al. 2019

horts

View full text Add to dashboard Cite

show abstract

“…Molecular markers are useful in identifying and isolating genes controlling disease resistance, or genes controlling fruit quality (Cobos et al, 2005;Liebhard et al, 2003;Mokrani et al, 2002;Soriano et al, 2005). As in other crop plants, including coffee (Coffea arabica L.) (Herrera et al, 2002) and banana (Musa L.) (Nwakanma et al, 2003), molecular markers can be useful in identifying and in selecting for triploid hybrid plants of watermelon.…”

mentioning

confidence: 99%

An Extended Linkage Map for Watermelon Based on SRAP, AFLP, SSR, ISSR, and RAPD Markers

Levi¹,

Thomas²,

Trebitsh³

et al. 2006

jashs

View full text Add to dashboard Cite

Seventy-one amplified fragment length polymorphism (AFLP), 93 sequence related amplified polymorphism (SRAP), and 14 simple sequence repeat (SSR) markers were used to extend an initial genetic linkage map for watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai]. The initial map was based on 151 randomly amplified polymorphic DNA (RAPD) and 30 and inter-simple sequence repeat (ISSR) markers. A testcross population previously used for mapping of RAPD and ISSR markers was used in this study: {plant accession Griffin 14113 [C. lanatus var. citroide (L.H. Bailey) Mansf.] × the watermelon cultivar New Hampshire Midget (C. lanatus var. lanatus)} × PI 386015 [C. colocynthis (L.) Schrad.]. The linkage map contains 360 DNA markers distributed on 19 linkage groups, and covers a genetic distance of 1976 cM with an average distance of 5.8 cM between two markers. A genomic DNA clone representing 1-amino-cyclopropane-1-carboxylic acid (ACC-) synthase gene, involved in ethylene biosynthesis, was also mapped. As in previous mapping studies for watermelon, a large number of AFLP and SRAP markers were skewed away from the 1:1 segregation ratio, and had to be excluded from the final mapping analysis. The stringent mapping criteria (JoinMap 3.0 mapping program) produced linkage groups with marker order consistent with those reported in previous mapping study for watermelon.

show abstract

“…In coffee, amplified fragment length polymorphism and simple sequence repeats have been successfully used to analyze the introgression of DNA fragments from Coffea canephora and Coffea liberica into C. arabica (Lashermes et al, 2000;Prakash et al, 2002;Herrera et al, 2002a). Currently, it is not possible to rely those markers with undesirable genes.…”

Section: Introductionmentioning

confidence: 99%

Stability across environments of the coffee variety near infrared spectral signature

Posada¹,

Ferrand

Davrieux

et al. 2008

Heredity

Self Cite

View full text Add to dashboard Cite

Previous study on food plants has shown that near infrared (NIR) spectral methods seem effective for authenticating coffee varieties. We confirm that result, but also show that inter-variety differences are not stable from one harvest to the next. We put forward the hypothesis that the spectral signature is affected by environmental factors. The purpose of this study was to find a way of reducing this environmental variance to increase the method's reliability and to enable practical application in breeding. Spectral collections were obtained from ground green coffee samples from multilocation trials. Two harvests of bean samples from 11 homozygous introgressed lines, and the cv 'Caturra' as the control, supplied from three different sites, were compared. For each site, squared Euclidean distances among the 12 varieties were estimated from the NIR spectra. Matrix correlation coefficients were assessed by the Mantel test. We obtained very good stability (high correlations) for intervariety differences across the sites when using the two harvests data. If only the most heritable zones of the spectrum were used, there was a marked improvement in the efficiency of the method. This improvement was achieved by treating the spectrum as succession of phenotypic variables, each resulting from an environmental and genetic effect. Heritabilities were calculated with confidence intervals. A near infrared spectroscopy signature, acquired over a set of harvests, can therefore effectively characterize a coffee variety. We indicated how this typical signature can be used in breeding to assist in selection.

show abstract

Gene introgression into Coffea arabica by way of triploid hybrids (C. arabica × C. canephora)

Cited by 28 publications

References 24 publications

Analysis of Genome Size of Sixteen Coffea arabica Cultivars Using Flow Cytometry

Analysis of Genome Size of Sixteen Coffea arabica Cultivars Using Flow Cytometry

An Extended Linkage Map for Watermelon Based on SRAP, AFLP, SSR, ISSR, and RAPD Markers

Stability across environments of the coffee variety near infrared spectral signature

Contact Info

Product

Resources

About