Gene Nanovector for Genome Therapy

Coexpression of TP53, BIM, and PTEN Enhances the Therapeutic Efficacy of Non-Small-Cell Lung Cancer

“…The branch-PCR technology could assemble the linear DNA cassettes into a nanoscale structure with a biocompatible size . If possible, at the same transfection dosage, the complete release amount of linear gene cassettes by the gene nanovector would also be in principle comparable to the total amount of the transfected linear DNA.…”

“…The branch-PCR technology could assemble the linear DNA cassettes into a nanoscale structure with a biocompatible size. 52 If possible, at the same transfection dosage, the complete release amount of linear gene cassettes by the gene nanovector would also be in principle comparable to the total amount of the transfected linear DNA. Under the same transfection dosage, for its serum instability, linear DNA might induce a similar or less effect on the cell growth state than gene nanovectors, which relies on the transfection amount, combinations of different genes, and cell confluence ratio for transfection.…”

“…In our previous work, we used tribranched primers and a linear DNA template to develop a branch-PCR method to assemble a series of nonviral gene nanovectors, which were compatible with diverse gene regulation technologies (gene expression, gene silencing, and genome editing). − This kind of gene nanovector harbors high DNA capacity, serum stability, and gene expression function. We also noted that the gene nanovector size could be adjusted by relying on the gene sequence length, which allows for balancing the nanovector size with the capacity needed to effectively deliver and express the cargo genes.…”