Gene networks and toxicity pathways induced by acute cadmium exposure in adult largemouth bass (Micropterus salmoides)

Mehinto, Alvine C.; Prucha, Melinda S.; Collí-Dulá, Reyna Cristina; Kroll, Kevin J.; Lavelle, Candice M.; Barber, David S.; Vulpe, Chris D.; Denslow, Nancy D.

doi:10.1016/j.aquatox.2014.04.004

Cited by 50 publications

(17 citation statements)

References 47 publications

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“…Previous work generated a transcriptome from M. salmoides from the liver, gonad and brain tissues using 454 sequencing. This previous effort captured 7395 annotated genes, which, along with un-annotated features, were used to develop a toxicology-focused Agilent microarray (Garcia-Reyero et al 2008;Mehinto et al 2014;Richter et al 2014). Contig sequences from this project are not publicly available and were short in length.…”

Section: Transcriptome Sequencing Assembly and Annotationmentioning

confidence: 99%

Discovery and validation of gene‐linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus)

Gowan

Anil

et al. 2014

Molecular Ecology Resources

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Efforts to improve recreational fisheries have included widespread stocking of Micropterus floridanus outside its native range of peninsular Florida. Hybridization of Florida bass (M. floridanus) with largemouth bass (Micropterus salmoides) has now dramatically expanded beyond a naturally occurring intergrade zone in the southeast U.S. In recent years, there has been growing interest in protecting the genetic integrity of native basses and assessing the impact and nature of M. salmoides/M. floridanus introgression from the standpoint of hatchery and sport-fishery managers, fish biologists, ecologists and evolutionary biologists. Here, we conducted RNA-seq-based sequencing of the transcriptomes of M. salmoides, M. floridanus and their F1 hybrid and identified a set of 3674 SNP markers with fixed-allelic differences from 2112 unique genes. We then developed a subset of 25 of these markers into a single diagnostic multiplex assay and validated its capacity for assessing integrity and hybridization in hatchery and wild populations of largemouth and Florida bass. The availability of this resource, high-quality transcriptomes and a large set of gene-linked SNPs, should greatly facilitate functional and population genomics studies in these key species and allow the identification of traits and processes under selection during introgressive hybridization.

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Section: Transcriptome Sequencing Assembly and Annotationmentioning

confidence: 99%

Discovery and validation of gene‐linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus)

Gowan

Anil

et al. 2014

Molecular Ecology Resources

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confidence: 99%

“…Heavy metal contamination in aquatic environments, particularly in freshwater systems, has posed severe risks to organisms 1 2 3 4 5 . Cadmium (Cd), a non-essential but toxic heavy metal, is a worldwide pollutant in aquatic systems 3 4 5 6 . Since Cd has a long biological half-life, its toxic effects can be accumulated and bio-amplified through the food web 3 4 7 8 9 10 .…”

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confidence: 99%

Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

Sun

Liu

et al. 2016

Sci Rep

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Cadmium (Cd) pollution is a serious global problem, which causes irreversible toxic effects on animals. Freshwater crab, Sinopotamon henanense, is a useful environmental indicator since it is widely distributed in benthic habitats whereby it tends to accumulate Cd and other toxicants. However, its molecular responses to Cd toxicity remain unclear. In this study, we performed transcriptome sequencing and gene expression analyses of its hepatopancreas with and without Cd treatments. A total of 7.78 G clean reads were obtained from the pooled samples, and 68,648 unigenes with an average size of 622 bp were assembled, in which 5,436 were metabolism-associated and 2,728 were stimulus response-associated that include 380 immunity-related unigenes. Expression profile analysis demonstrated that most genes involved in macromolecular metabolism, oxidative phosphorylation, detoxification and anti-oxidant defense were up-regulated by Cd exposure, whereas immunity-related genes were down-regulated, except the genes involved in phagocytosis were up-regulated. The current data indicate that Cd exposure alters gene expressions in a concentration-dependent manner. Therefore, our results provide the first comprehensive S.henanense transcriptome dataset, which is useful for biological and ecotoxicological studies on this crab and its related species at molecular level, and some key Cd-responsive genes may provide candidate biomarkers for monitoring aquatic pollution by heavy metals.

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“…Though the genes related to Cd response have been widely studied in many vertebrate and invertebrate [19,20,21], only a few genes of spider responding to heavy metals have been reported previously. The wolf spider Pardosa pseudoannulata ( P. pseudoannulata ) is one of the most common species of wandering spiders in agricultural fields in China, and acts as one of the most important natural enemies to reduce pest populations, such as rice plant hoppers and leafhoppers [22].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure

Wang

et al. 2016

IJMS

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Pardosa pseudoannulata is one of the most common wandering spiders in agricultural fields and a potentially good bioindicator for heavy metal contamination. However, little is known about the mechanisms by which spiders respond to heavy metals at the molecular level. In the present study, high-throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and to identify differentially expressed genes (DEGs) after cadmium exposure. We obtained 60,489 assembled unigenes, 18,773 of which were annotated in the public databases. A total of 2939 and 2491 DEGs were detected between the libraries of two Cd-treated groups and the control. Functional enrichment analysis revealed that metabolism processes and digestive system function were predominately enriched in response to Cd stress. At the cellular and molecular levels, significantly enriched pathways in lysosomes and phagosomes as well as replication, recombination and repair demonstrated that oxidative damage resulted from Cd exposure. Based on the selected DEGs, certain critical genes involved in defence and detoxification were analysed. These results may elucidate the molecular mechanisms underlying spiders’ responses to heavy metal stress.

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Gene networks and toxicity pathways induced by acute cadmium exposure in adult largemouth bass (Micropterus salmoides)

Cited by 50 publications

References 47 publications

Discovery and validation of gene‐linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus)

Discovery and validation of gene‐linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus)

Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure

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