Gene networks underlying faster flowering induction in response to far-red light

Navarro, Maria Pazos; Ribalta, Federico M.; Hurgobin, Bhavna; Croser, Janine; Kaur, Parwinder

doi:10.1101/234161

Cited by 5 publications

(5 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another recent study with an annual subterranean clover ( Trifolium subterraneum L .) showed that several putative flowering‐promoting genes were upregulated under FR‐enriched light, such as orthologs of FLOWERING LOCUS T ( FT ) and CONSTANS ‐like ( COL ) genes (Pazos Navarro et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…Among others, they identified orthologs of PHYTOCHROME INTERACTING FACTOR 3 (PIF3) and B-BOX PROTEIN 19 (BBX19), alongside several heat-shock protein orthologs. Another recent study with an annual subterranean clover (Trifolium subterraneum L.) showed that several putative flowering-promoting genes were upregulated under FR-enriched light, such as orthologs of FLOWERING LOCUS T (FT) and CONSTANS-like (COL) genes (Pazos Navarro et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Shade delays flowering in Medicago sativa

et al. 2019

View full text Add to dashboard Cite

Summary Shade‐intolerant plants respond to the decrease in the red (R) to far‐red (FR) light ratio (R:FR) occurring under shade by elongating stems and petioles and by re‐positioning leaves, in a race to outcompete neighbors for the sunlight resource. In some annual species, the shade avoidance syndrome (SAS) is accompanied by the early induction of flowering. Anticipated flowering is viewed as a strategy to set seeds before the resources become severely limiting. Little is known about the molecular mechanisms of SAS in perennial forage crops like alfalfa (Medicago sativa). To study SAS in alfalfa, we exposed alfalfa plants to simulated shade by supplementing with FR light. Low R:FR light produced a classical SAS, with increased internode and petiole lengths, but unexpectedly also with delayed flowering. To understand the molecular mechanisms involved in uncoupling SAS from early flowering, we used a transcriptomic approach. The SAS is likely to be mediated by increased expression of msPIF3 and msHB2 in low R:FR light. Constitutive expression of these genes in Arabidopsis led to SAS, including early flowering, strongly suggesting that their roles are conserved. Delayed flowering was likely to be mediated by the downregulation of msSPL3, which promotes flowering in both Arabidopsis and alfalfa. Shade‐delayed flowering in alfalfa may be important to extend the vegetative phase under suboptimal light conditions, and thus assure the accumulation of reserves necessary to resume growth after the next season.

show abstract

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Shade delays flowering in Medicago sativa

et al. 2019

View full text Add to dashboard Cite

show abstract

“…This QTL was located at the beginning of chromosome 6 (Figures 2 and 4a) with a significant SNP in the 5′ untranslated region of the first of two lentil FTb ortholog genes located in tandem at the top of chromosome 6. FTb genes are known flowering time genes related to photoperiod response in several legumes including lentil (Hecht et al., 2011; Ortega et al., 2019; Pazos‐Navarro et al., 2018; Weller & Ortega, 2015; Yuan et al., 2021). In all these species, the expression of FTb genes show a strong long‐day requirement and therefore the effect of a FTb allele with (a) a mutation altering their expression pattern would be more relevant in short‐day environments (Mediterranean, southern Asia) or (b) a mutation impairing or enhancing its floral inductive capacity would be more evident in locations where photoperiodic requirements are met such as temperate environments.…”

Section: Resultsmentioning

confidence: 99%

Focusing the GWAS Lens on days to flower using latent variable phenotypes derived from global multienvironment trials

et al. 2022

View full text Add to dashboard Cite

Adaptation constraints within crop species have resulted in limited genetic diversity in some breeding programs and areas where new crops have been introduced, for example, for lentil (Lens culinaris Medik.) in North America. An improved understanding of the underlying genetics involved in phenology-related traits is valuable knowledge to aid breeders in overcoming limitations associated with unadapted germplasm and expanding their genetic diversity by introducing new, exotic material.We used a large, 18 site-year, multienvironment dataset phenotyped for phenologyrelated traits across nine locations and over 3 yr along with accompanying latent variable phenotypes derived from a photothermal model and principal component analysis (PCA) of days from sowing to flower (DTF) data for a lentil diversity panel (324 accessions), which has also been genotyped with an exome capture array. Genome-wide association studies (GWAS) on DTF across multiple environments helped confirm associations with known flowering-time genes and identify new quantitative trait loci (QTL), which may contain previously unknown flowering time genes. Additionally, the use of latent variable phenotypes, which can incorporate environmental data such as temperature and photoperiod as both GWAS traits and as covariates, strengthened associations, revealed additional hidden associations, and alluded to potential roles of the associated QTL. Our approach can be replicated with other crop species, and the results from our GWAS serve as a resource for further exploration into the complex nature of phenology-related traits across the major growing environments for cultivated lentil.

show abstract

“…This QTL was located at the beginning of chromosome 6 ( Figure 2, Figure 4a ), with a significant SNP in the 5’ UTR of the first of two lentil FTb orthologs genes located in tandem at the top of chromosome 6. FTb genes are known flowering time genes related to photoperiod response in several legumes, including lentil (24, 53, 56, 58, 59). In all these species, the expression of FTb genes show a strong long day requirement, and therefore the effect of a FTb allele (i) with a mutation altering their expression pattern would be more relevant in SD environments (Mediterranean, South Asian) or (ii) with a mutation impairing or enhancing its floral inductive capacity would be more evident in locations where photoperiodic requirements are met, such as temperate environments.…”

Section: Resultsmentioning

confidence: 99%

Focusing the GWASLenson days to flower using latent variable phenotypes derived from global multi-environment trials

Neupane

Wright

Martinez

et al. 2022

Preprint

View full text Add to dashboard Cite

Adaptation constraints within crop species have resulted in limited genetic diversity in some breeding programs and/or areas where new crops have been introduced, e.g., lentil (Lens culinaris Medik.) in North America. An improved understanding of the underlying genetics involved in phenology-related traits is valuable knowledge to aid breeders in overcoming limitations associated with unadapted germplasm and expanding their genetic diversity by introducing new, exotic material. We used a large, 18 site-year, multi-environment dataset, phenotyped for phenology-related traits across nine locations and over three years, along with accompanying latent variable phenotypes derived from a photothermal model and principal component analysis (PCA) of days from sowing to flower (DTF) data for a lentil diversity panel (324 accessions) which has also been genotyped with an exome capture array. Genome-wide association studies (GWAS) on DTF across multiple environments helped confirm associations with known flowering time genes and identify new quantitative trait loci (QTL), which may contain previously unknown flowering time genes. Additionally, the use of latent variable phenotypes, which can incorporate environmental data such as temperature and photoperiod as both GWAS traits and as covariates, strengthened associations, revealed additional hidden associations, and alluded to potential roles of the associated QTL. Our approach can be replicated with other crop species, and the results from our GWAS serve as a resource for further exploration into the complex nature of phenology-related traits across the major growing environments for cultivated lentil.

show abstract

Gene networks underlying faster flowering induction in response to far-red light

Cited by 5 publications

References 63 publications

Shade delays flowering in Medicago sativa

Shade delays flowering in Medicago sativa

Focusing the GWAS Lens on days to flower using latent variable phenotypes derived from global multienvironment trials

Focusing the GWASLenson days to flower using latent variable phenotypes derived from global multi-environment trials

Contact Info

Product

Resources

About