Gene packaging with lipids, peptides and viruses inhibits transfection by electroporation in vitro

An electroporation microchip system for the transfection of zebrafish embryos using quantum dots and GFP genes for evaluation

“…The EP technique employs one or several short electrical impulses to break down the dielectric layer on the cell membrane and thereby creates multiple transient pores allowing the entry of DNA into the cells (Tryfona and Bustard 2006). This way, foreign DNAs can be introduced into cells, thereby allowing the batch production of transgenics (Coulberson et al 2003).…”

“…In addition, it can be used to deliver specific extra-cellular genes into targets within a very short time. Nowadays, the EP method is used in cancer research (Canatella and Prausnitz 2001;Jamieson et al 1989;Nishi et al 1996;Yang and Sun 1995), gene packaged with lipids, peptides and viruses transfection (Coulberson et al 2003;Tryfona and Bustard 2006), and drug delivery (Canatella and Prausnitz 2001). In addition, the EP method is successful in transgenic treatments in mice (Lavitrano et al 1989;Marti et al 2004;Neumann et al 1982;Nishi et al 1996), silkworm (Shamila and Mathavan 1998), sea urchin (Larochelle and Epel 1991), cattle (Gagne et al 1991), chick (Nakamura andFunahashi 2001), abalone (Powers et al 1995), and fish (Rambabu et al 2005;Connaughton et al 2004).…”

Varying the Unsaturation in N4,N9-Dioctadecanoyl Spermines: Nonviral Lipopolyamine Vectors for More Efficient Plasmid DNA Formulation

“…Within this broad term, we are concentrating on lipopolyamines composed of a lipophilic steroid attached either to a polyamine chain (4Y8) or to a single or double long-carbon chain covalently bound to a polyamine, e.g., spermine (N 1 ,N 12 -diamino-4,9-diazadodecane) (9Y11). Other research groups are investing in a variety of alternative approaches classified under the NVGT umbrella, including the following: naked DNA (12), gene gun (bound to gold particles) (13,14), electroporation (15), polycationmediated DNA delivery, and the use of a wide variety of cationic lipids (lipoplexes) (16), e.g., bolaamphiphiles (17), and cationic polymers (polyplexes) (18Y20) [for reviews, see (21Y25)]. For gene therapy to realize its potential and become an efficient medicine for the treatment of diseases such as cancer, cystic fibrosis, inflammation, or for vaccination, key obstacles must be overcome.…”

Comparative analysis of the methods of drug and protein delivery for the treatment of cancer, genetic diseases and diagnostics

“…The electroporation was applied to DU145 prostate cancer cells, incubated with GFP-encoded DNA plasmid, either naked or packaged with cationic lipid (Lipofectin), polycationic peptide (salmon protamine) or retroviral vectors (Moloney murine leukemia viruses), and then assayed for gene expression and cell viability. The combination of electroporation with chemicals as cationic lipids, or viral vectors does not improve the gene transfection in vitro (Coulberson, 2003). The proteins cytochrome c, granzyme-B, caspase-8, known to activate caspase-family cell death proteases, were introduced into human leukemia and lymphoma cell lines, freshly isolated lymphocytes and leukemia cells to contrast the status of various caspase activation pathways, related to pathological defects in the regulation of apoptosis that exist in individual patient specimens (Eksioglu-Demiralp, 2003).…”