Gene polymorphisms in calcium-calmodulin pathway: Focus on cardiovascular disease

Beghi, Sofia; Cavaliere, Francesca; Buschini, Annamaria

doi:10.1016/j.mrrev.2020.108325

Cited by 7 publications

(17 citation statements)

References 136 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The peptide sequence coverage was 60% for Macrobrachium rosenbergii calmodulin (accession AOA3S8FSK5). Calmodulins have a highly conserved molecular structure encoded in their protein sequences, and their biological activities are manifold, in addition to participating in various intracellular signal transduction pathways [ 49 ]. Since purified O. oratoria MT-1 exhibited high homology with calmodulin, it can be inferred that O. oratoria MT-1 may also possess calmodulin-like biological activities.…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization and In Vitro Antioxidant Activity of Metallothionein from Oratosquilla oratoria

Mei

Zhang

et al. 2022

Molecules

View full text Add to dashboard Cite

We report here the purification of a novel metal-binding protein from Oratosquilla oratoria (O. oratoria MT-1) by gel and ion-exchange chromatography. SDS-PAGE and MALDI-TOF analyses demonstrated that isolated O. oratoria MT-1 was of high purity with a molecular weight of 12.4 kDa. The fluorescence response to SBD-F derivatives revealed that O. oratoria MT-1 contained a large number of sulfhydryl groups, which is a general property of metallothioneins. Zn and Cu metal stoichiometries for O. oratoria MT-1 were 3.97:1 and 0.55:1, respectively. The proportion of cysteine (Cys) residues in the amino acid composition was 32.69%, and aromatic amino acids were absent. The peptide sequence coverage with Macrobrachium rosenbergii calmodulin (accession AOA3S8FSK5) was 60%. Infrared spectroscopy of O. oratoria MT-1 revealed two obvious peaks at absorption frequencies for the amide I band and the amide II band. CD spectra revealed that the secondary structure was mainly composed of random coil (57.6%) and β-sheet (39.9%). An evaluation of in vitro antioxidant activity revealed that isolated O. oratoria MT-1 has strong reducing activities, exhibiting scavenging rates for DPPH and OH of 77.8% and 75.8%, respectively (IC50 values 0.57 mg/mL and 1.1 mg/mL). O. oratoria MT-1 may be used as a functional additive in cosmetics, health foods, and medical products, as well as a reference material for quantitative analysis of metallothionein in such products.

show abstract

Section: Resultsmentioning

confidence: 99%

Structural Characterization and In Vitro Antioxidant Activity of Metallothionein from Oratosquilla oratoria

Mei

Zhang

et al. 2022

Molecules

View full text Add to dashboard Cite

show abstract

“…In a previous review, we discussed the several SNPs associated with CVDs in genes involved in the calcium/calmodulin pathway [14][15][16][17][18][19][20][21]. In particular, this study focused on some downstream genes to calmodulin, the first calcium sensor and signal transducer.…”

Section: Discussionmentioning

confidence: 99%

“…Within the different calcium signaling pathways, calmodulin has a central role: its function is to integrate the calcium signal and to transduce it to other downstream enzymes, such as NOS, PPP3C as well as the proteins of the CAMK subfamily. In a previous review [14] we focused our attention on the role played by genetic variants in these family of proteins in the development of CVDs [15][16][17][18][19][20][21]. Here we focus on CAMKK1, a calcium/calmodulin-dependent kinase kinase 1, functionally located upstream to others CAMK kinases involved in the transduction of calcium signaling.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polymorphism rs7214723 in CAMKK1: a new genetic variant associated with cardiovascular diseases

et al. 2021

Self Cite

View full text Add to dashboard Cite

Cardiovascular diseases (CVDs) are leading cause of death worldwide. CVDs have a complex etiology due to the several factors underlying its development including environment, lifestyle, and genetics. Given the role of calcium signal transduction in several CVDs, we investigated via PCR-RFLP the single nucleotide polymorphism (SNP) rs7214723 within the CAMKK1 gene coding for the Ca2 + / calmodulin dependent protein kinase kinase I. The variant rs7214723 causes a E375G substitution within the kinase domain of CAMKK1. A cross-sectional study was conducted on 300 cardiac patients. RFLP-PCR technique was applied, and statistical analysis was performed to evaluate genotypic and allelic frequencies and to identify an association between SNP and risk of developing specific CVD. Genotype and allele frequencies for rs7214723 were statistically different between cardiopathic and several European reference populations. A logistic regression analysis adjusted for gender, age, diabetes, hypertension, BMI and previous history of malignancy was applied on cardiopathic genotypic data and no association was found between rs7214723 polymorphism and a risk of developing specific CAD. These results suggest the potential role of rs7214723 in CVD susceptibility as a possible genetic biomarker.

show abstract

“…It also has notable roles in cell proliferation, cyclic nucleotide metabolism, cellular Ca 2+ metabolism, gene expression, muscle contraction, and proteolysis (Sharma and Parameswaran, 2018 ). CALM activity is closely related to MI, arrhythmia, and hyperthyroid CDs (Beghi et al, 2020 ; Chazin and Johnson, 2020 ; Hou et al, 2020 ) and is involved in the occurrence and development of neurological diseases (O'Day, 2020 ). Both TPM and CALM proteins played important roles in the cardiovascular system and nervous system at the same time.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Sequencing of Stellate Ganglions in Rabbits With Myocardial Infarction

et al. 2021

View full text Add to dashboard Cite

The stellate ganglion (SG) of the autonomic nervous system plays important role in cardiovascular diseases (CDs). Myocardial infarction (MI) is associated with sustained increasing cardiac sympathetic nerve activity. Expressions and functions of proteins in SG tissue after MI are remaining unclear. This study is to explore the expression characteristics of proteins in SGs associated with MI. Japanese big-ear white rabbits (n = 22) were randomly assigned to the control group and MI group. The MI model was established by left anterior descending coronary artery ligation and confirmed by serum myocardial enzymes increasing 2,3,5-triphenyltetrazolium (TTC) staining and echocardiography. The expressions of proteins in rabbit SGs after MI were detected using tandem mass tags (TMT) quantitative proteomic sequencing. There were 3,043 credible proteins were predicted in rabbit SG tissues and 383 differentially expressed proteins (DEPs) including 143 upregulated and 240 downregulated proteins. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the DEPs involved in adrenergic signaling in cardiomyocytes, positive regulation of ERK1 and ERK2 cascade, and other biological processes. Three kinds of proteins directly correlated to CDs were selected to be validated by the subsequent western blot experiment. This study first identified the characterization of proteins in rabbit SG after MI, which laid a solid foundation for revealing the mechanism of roles of SG on the MI process.

show abstract

Gene polymorphisms in calcium-calmodulin pathway: Focus on cardiovascular disease

Cited by 7 publications

References 136 publications

Structural Characterization and In Vitro Antioxidant Activity of Metallothionein from Oratosquilla oratoria

Structural Characterization and In Vitro Antioxidant Activity of Metallothionein from Oratosquilla oratoria

Polymorphism rs7214723 in CAMKK1: a new genetic variant associated with cardiovascular diseases

Proteomic Sequencing of Stellate Ganglions in Rabbits With Myocardial Infarction

Contact Info

Product

Resources

About