Gene polymorphisms of adiponectin and leptin receptor are associated with early onset of type 2 diabetes mellitus in the Taiwanese population

Abstract

Objective: Adipocytokine genes encoding adiponectin (ADIPOQ) and the leptin receptor (LEPR) affect glucose and fatty acid metabolism. The purpose of this study was to examine the association between early-onset type 2 diabetes mellitus (T2DM) and variability within these two genes in the Han Chinese population of Taiwan. Subjects: A cross-sectional study of 999 patients from the Han Chinese population of Taiwan with early-onset T2DM (n ¼ 264; age at diagnosis, 20 to o45 years) and late-onset T2DM (n ¼ 735; age at diagnosis, X45 years) was performed. Blood samples from T2DM patients were taken for DNA extraction, and levels of serological markers were measured at enrollment. Seven single-nucleotide polymorphisms (SNPs) were selected for genotyping (three SNPs in AIDPOQ and four SNPs in LEPR) by polymerase chain reaction in each patient. Results: Polymorphisms at the position rs10937273 in ADIPOQ and at the positions rs1892534 and rs2211651 in LEPR were statistically associated with early-onset T2DM (P ¼ 0.0246, 0.0014 and 0.0012, respectively). C-reactive protein levels were significantly different among the early-onset T2DM patients with different genotypes at the SNPs rs1892534 and rs2211651 in LEPR (P ¼ 0.003 and P ¼ 0.004, respectively). In addition, fasting glucose levels were also significantly different among different genotypes at the SNP rs1892534 in LEPR (P ¼ 0.038). Conclusion: We conclude that the polymorphisms in the adipocytokine genes ADIPOQ and LEPR are significantly associated with the age at diagnosis of T2DM in the Han Chinese population of Taiwan.

Association of LEPR Gln223Arg polymorphism with T2DM: A meta-analysis

“…There was no obvious association between LEPR Gln223Arg polymorphism and T2DM in Asians either (G vs. A, OR = 1.08, 95% CI 0.75 to 1.57, P = 0.68) (Fig. 3) [5][6][7][8][10][11][12][13]15]. …”

“…Sixteen individual studies with 7827subjects from 14 publications were finally included into the meta-analysis [5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Among those 16 studies, six studies were performed in Caucasian countries, while the other 10 studies were performed in Asians (Table 1).…”

Association of maternal and fetal LEPR common variants with maternal glycemic traits during pregnancy

“…Significant association between SNP 23 (rs1137100: Lys109Arg) and FPG levels were observed in a few studies 25, 26 , but more studies has documented that SNP 23 was not associated with T2DM 13, 27, 34 , early-onset T2DM 35 , HOMA1-IR 34 , fasting glucose 34–36 , insulin 27, 34 , leptin 27 and sLEPR levels 32 . Similarly, in our study, maternal and fetal SNP 23 as well as maternal-fetal genotype combination of SNP 23 were not associated with gestational glycemic traits (data not shown).…”

Sequence Variants ofADIPOQand Association with Type 2 Diabetes Mellitus in Taiwan Chinese Han Population

“…ADIPOQ polymorphisms and T2DM or diabetic nephropathy had also been evaluated from many populations: for example, the European-derived [17, 18], the Asian [14, 16, 40], the non-European South African [43], and the African American [44] populations. Still, no difference appeared between these SNPs and T2DM in the Taiwanese population [45]. Gao et al indicated G allele frequency of ADIPOQ SNP (rs2241766) as prevalent in metabolic syndrome patients versus control groups in the Chinese Han population [10], which demonstrably augments risk of T2DM in the Chinese population [10].…”