Gene polymorphisms of SFTPB rs7316, rs9752 and PAOX rs1046175 affect the diagnostic value of plasma Pro‐SFTPB and DAS in Chinese Han non–small‐cell lung cancer patients

Abstract

Plasma pro‐surfactant protein B (pro‐SFTPB) and N1,N12‐diacetylspermine (DAS) can be used as markers for the diagnosis of non–small‐cell lung carcinoma (NSCLC). Whether the genetic diversity affects the application value of Pro‐SFTPB and DAS as a diagnostic marker for NSCLC is still unknown. This study aims to explore the relationship between SFTPB rs7316, rs9752 and PAOX rs1046175 gene polymorphisms and the diagnostic value of plasma Pro‐SFTPB and DAS in patients with Chinese Han lung cancer. SFTPB rs7316, rs9752 and PAOX rs1046175 genotypes were analyzed by direct sequencing in 425 patients with NSCLC and 425 controls, and the levels of Pro‐SFTPB and DAS in plasma were determined by enzyme‐linked immunosorbent assay (ELISA). The area under the curve (AUC) of the SFTPB rs7316 locus TT genotype for the diagnosis of NSCLC was 0.758, and the AUC of the TC/CC genotype for the diagnosis of NSCLC was 0.872. The AUC of the SFTPB rs9752 locus GG genotype for the diagnosis of NSCLC was 0.935, and the AUC of the GC/CC genotype for the diagnosis of NSCLC was 0.648. The AUC of the PAOX rs1046175 locus GG for the diagnosis of NSCLC was 0.669, and the AUC of the GC/CC genotype for the diagnosis of NSCLC was 0.749. In conclusion, SFTPB rs7316, rs9752, and PAOX rs1046175 gene polymorphisms affect the diagnostic value of plasma Pro‐SFTPB and DAS in patients with Chinese Han NSCLC.

The Common Haplotype GATGACA in Surfactant-Protein B Gene Is Protective for Respiratory Distress Syndrome in Preterm Neonates

“…SNP rs9752 is located at 3′UTR. Recently, the association of recessive homozygotes harboring the CC genotype and heterozygotes with the CT genotype has been demonstrated with reduced plasma pro-SFTPB in non-small cell lung cancer patients ( 25 ), which could also indicate some functional effects of this SNP on gene expression.…”

Pulmonary surfactant‐associated protein B regulates prostaglandin‐endoperoxide synthase‐2 and inflammation in chronic obstructive pulmonary disease

“…It is synthesized exclusively by type 2 alveolar pneumocytes and non-ciliated bronchiolar cells and has various roles, including functions relating to the immune system as well as a reduction of surface tension at the air-liquid interface (Seeger et al, 1992;Ikegami et al, 2005). Variants of SFTPB have been associated with several lung diseases, including acute respiratory distress syndrome (Gandhi et al, 2020), neonatal respiratory distress (Magnani & Donn, 2020) and lung cancer (Guida et al, 2018;Wang et al, 2019). However, most studies have so far only investigated gene polymorphism.…”