Gene pyramiding for elite tomato genotypes against ToLCV (Begomovirus spp.), late blight (Phytophthora infestans) and RKN (Meloidogyne spp.) for northern India farmers

Kumar, Ashish; Jindal, S. K.; Dhaliwal, M. S.; Sharma, Abhishek; Kaur, Sukhjeet; Jain, Sandeep

doi:10.1007/s12298-019-00700-5

Cited by 28 publications

(10 citation statements)

References 30 publications

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“…While these responses may be affected by RNA-editing sites or factors where amino acid changes affect the codon, like SNP variations are responsible for the opposite response, which may not allow gene upregulation ( Wang et al, 2021 ). The differential expression of NLRs for disease tolerance in wild and moderately susceptible, and tolerant and susceptible cultivated genotypes, suggests the key role of the overexpression of these antifungal genes and gene pyramiding of NLRs against disease management in managing disease ( Kumar et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide characterization of the NLR gene family in tomato (Solanum lycopersicum) and their relatedness to disease resistance

Bashir

Rehman²,

Zaman³

et al. 2022

Front. Genet.

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Nucleotide-binding leucine-rich-repeat receptors (NLR), the largest group of genes associated with plant disease resistance (R), have attracted attention due to their crucial role in protecting plants from pathogens. Genome-wide studies of NLRs have revealed conserved domains in the annotated tomato genome. The 321 NLR genes identified in the tomato genome have been randomly mapped to 12 chromosomes. Phylogenetic analysis and classification of NLRs have revealed that 211 genes share full-length domains categorized into three major clades (CNL, TNL, and RNL); the remaining 110 NLRs share partial domains and are classified in CN, TN, and N according to their motifs and gene structures. The cis-regulatory elements of NLRs exhibit the maximum number of these elements and are involved in response to biotic and abiotic stresses, pathogen recognition, and resistance. Analysis of the phylogenetic relationship between tomato NLRs and orthologs in other species has shown conservation among Solanaceae members and variation with A. thaliana. Synteny and Ka/Ks analyses of Solanum lycopersicum and Solanum tuberosum orthologs have underscored the importance of NLR conservation and diversification from ancestral species millions of years ago. RNA-seq data and qPCR analysis of early and late blight diseases in tomatoes revealed consistent NLR expression patterns, including upregulation in infected compared to control plants (with some exceptions), suggesting the role of NLRs as key regulators in early blight resistance. Moreover, the expression levels of NLRs associated with late blight resistance (Solyc04g007060 [NRC4] and Solyc10g008240 [RIB12]) suggested that they regulate S. lycopersicum resistance to P. infestans. These findings provide important fundamental knowledge for understanding NLR evolution and diversity and will empower the broader characterization of disease resistance genes for pyramiding through speed cloning to develop disease-tolerant varieties.

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Section: Discussionmentioning

confidence: 99%

Genome-wide characterization of the NLR gene family in tomato (Solanum lycopersicum) and their relatedness to disease resistance

Bashir

Rehman²,

Zaman³

et al. 2022

Front. Genet.

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“…DNA marker technology can tag novel resistant gene(s) efficiently and provide a straight forward path for identifying and transferring gene(s) into various crops (Gu et al, 2013 ; Patroti et al, 2019 ). In previous studies, MAS has been performed to few vegetable crops such as tomato for resistance to tomato leaf curl virus (ToLCV) (Kumar et al, 2014 ; Prasanna et al, 2014 ), late blight, and root knot nematode (Kumar et al, 2019 ); and in pepper for resistance to PVY, TSWV, and PMMoV (Özkaynak et al, 2014 ). Many QTLs/gene(s) have been reported for both black rot and downy mildew diseases in cauliflower; however, there has been no report of transferring these genes(s)/QTLs through MAS.…”

Section: Discussionmentioning

confidence: 99%

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

et al. 2021

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Cauliflower is an important extensively grown cool season vegetable in India. Black rot and downy mildew are major devastating diseases reducing yield and quality of the crop. To tackle these through host plant resistance, a marker-assisted backcross breeding method was followed to pyramid a black rot-resistant gene (Xca1bo) and a downy mildew-resistant gene (Ppa3) from donors BR-161 and BR-2, respectively, into the background of Pusa Meghna cauliflower cultivar. Marker-assisted backcross breeding was followed up to BC2 generation using SCAR marker ScOPO-04833 and SSR marker BoGMS0624 for black rot and downy mildew resistance genes in foreground selection, respectively. In background selection, at each stage of backcrossing, 47 parental polymorphic SSR markers were used. The graphical genotyping of the five two-gene (Xca1boXca1boPpa3Ppa3) homozygous BC2F2 plants showed an average recovery of 85.44% of the Pusa Meghna genome with highest genome recovery of 91.7%. The genome contribution of donor parents (BR-161 and BR-2) was 8.26 with 6.34% of residual heterozygousity. The backcross derived pyramided lines BC2F2:3-7-16 and BC2F2:3-7-33 showed high resistance to both the diseases and exhibited higher yield and vitamin C content as compared with recipient parent Pusa Meghna. It is, therefore, evident from this study that resistant genes can be introgressed successfully into a Pusa Meghna cultivar without any yield penalty, benefitting farmers with reduced input cost and consumers with chemical residue free produce. Besides, the pyramided lines carrying dominant resistant genes can be exploited in a hybridization programme to develop hybrid(s) in cauliflower.

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“…The disease severity and disease incidence were recorded at 45 days after inoculation as suggested by ALEGBEJO (1997) in the scale of 0-7 (0, 1, 3, 5 and 7). The disease incidence (DI) and disease severity or per cent disease index (PDI), t he coefficient of infection (CI) and decides the level of resistant reaction against ToLCV disease accordingly as mentioned by KUMAR et al, 2019. Artificial screening for late blight was conducted through the "detached leaf assay" during the month of February, 2018. In this method, detached young leaves of all the 122 lines including resistant (LBR-10) and susceptible (Punjab Chhuhara) checks were thoroughly washed with tap-water, air-dry and placed in plastic trays lined with moist blotting paper, with the adaxial surface facing upwards to make easy penetration of pathogen.…”

Section: Linesmentioning

confidence: 99%

Horticultural evaluation of advance breeding lines possessing different combinations of Ty and Ph genes in tomato (Solanum lycopersicum L.)

Kumar¹,

Jindal²,

Dhaliwal³

et al. 2019

Genetika

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Agro-statistics, India is the second largest tomato producer only after China in the world. Tomato crop is grown round the year due to wider adaptability but production is adversely affected due to high incidence of tomato leaf curl virus and late blight diseases. These pathogens have enormous capacity to generate new forms and control of these pathogen are mainly achieved by chemical approaches which is not safe for environment as well as for human beings and also add an extra cost in tomato production. Therefore resistance breeding is best approach to manage these types of diseases along with improvement of yield and quality parameters. Five genes viz. Ty-1, Ty-2, Ty-3, Ph-2 and Ph-3 were tried to pyramid from different parent through hybridization to achieve resistance against tomato leaf curl virus and late blight diseases. In this study, 122 advance breeding lines of tomato were evaluated in 2016-17 and 2017-18, through phenotypic and genotypic screening to identify best lines having resistance to both the diseases with better horticultural traits. Punjab Chhuhara cultivar was used as common susceptible check to both the diseases while PVB-4 and LBR-10 were used as resistant 772

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Gene pyramiding for elite tomato genotypes against ToLCV (Begomovirus spp.), late blight (Phytophthora infestans) and RKN (Meloidogyne spp.) for northern India farmers

Cited by 28 publications

References 30 publications

Genome-wide characterization of the NLR gene family in tomato (Solanum lycopersicum) and their relatedness to disease resistance

Genome-wide characterization of the NLR gene family in tomato (Solanum lycopersicum) and their relatedness to disease resistance

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

Horticultural evaluation of advance breeding lines possessing different combinations of Ty and Ph genes in tomato (Solanum lycopersicum L.)

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