Gene Pyramiding in Transgenic Plant Development: Approaches and Challenges

Rajput, Ruchika; Naik, Jogindra; Misra, Prashant; Trivedi, Prabodh Kumar; Pandey, Ashutosh

doi:10.1007/s00344-022-10760-9

Cited by 12 publications

(4 citation statements)

References 142 publications

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“…Importantly, the reduction of the Pm3f chlorotic leaf phenotype in the pyramided line suggests that the other Pm3 alleles contribute to the phenotype and are not simply silenced. Transgene silencing and suppression are phenomena which have been observed before when combining different transgenes or alleles and should be considered when breeding pyramided lines (Rajput et al 2022 ; Zorrilla-López et al 2013 ; Hurni et al 2014 ; Stirnweis et al 2014 ; Koller et al 2023 ). For resistance gene Pm3 particularly, functional suppression among different Pm3 alleles was previously shown in seedling infection tests when combined in double Pm3 -transgenic wheat lines (Stirnweis et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Breeding for durable resistance against biotrophic fungal pathogens using transgenes from wheat

Camenzind,

Koller,

Armbruster

et al. 2024

Mol Breeding

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Breeding for resistant crops is a sustainable way to control disease and relies on the introduction of novel resistance genes. Here, we tested three strategies on how to use transgenes from wheat to achieve durable resistance against fungal pathogens in the field. First, we tested the highly effective, overexpressed single transgene Pm3e in the background of spring wheat cultivar Bobwhite in a long-term field trial over many years. Together with previous results, this revealed that transgenic wheat line Pm3e#2 conferred complete powdery mildew resistance during a total of nine field seasons without a negative impact on yield. Furthermore, overexpressed Pm3e provided resistance to powdery mildew isolates from our worldwide collection when crossed into the elite wheat cultivar Fiorina. Second, we pyramided the four overexpressed transgenes Pm3a, Pm3b, Pm3d, and Pm3f in the background of cultivar Bobwhite and showed that the pyramided line Pm3a,b,d,f was completely resistant to powdery mildew in five field seasons. Third, we performed field trials with three barley lines expressing adult plant resistance gene Lr34 from wheat during three field seasons. Line GLP8 expressed Lr34 under control of the pathogen-inducible Hv-Ger4c promoter and provided partial barley powdery mildew and leaf rust resistance in the field with small, negative effects on yield components which might need compensatory breeding. Overall, our study demonstrates and discusses three successful strategies for achieving fungal disease resistance of wheat and barley in the field using transgenes from wheat. These strategies might confer long-term resistance if applied in a sustainable way.

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Section: Discussionmentioning

confidence: 99%

Breeding for durable resistance against biotrophic fungal pathogens using transgenes from wheat

Camenzind,

Koller,

Armbruster

et al. 2024

Mol Breeding

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“…Plant transformation plays a key role in plant improvement by introducing beneficial exogenous genes or by silencing the expression of endogenous genes in crop plants ( Kumar et al., 2020 ). Transgenic crops have one or more useful traits, such as herbicide tolerance, insect resistance, abiotic stress tolerance, disease resistance, and nutritional improvement ( Rajput et al., 2022 ). With the development of biotechnology, kiwifruit has also made great progress in genetic transformation, with the translation of some exogenous target genes.…”

Section: Discussionmentioning

confidence: 99%

An effective method for establishing a regeneration and genetic transformation system for Actinidia arguta

Yao,

Kong,

Lei

et al. 2023

Front. Plant Sci.

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The all-red A. arguta (Actinidia arguta) is an anthocyanin-rich and excellent hardy fruit. Many studies have focused on the green-fleshed A. arguta, and fewer studies have been conducted on the all-red A. arguta. Here we reported a regeneration and Agrobacterium-mediated transformation protocol by using leaves of all-red A. arguta as explants. Aseptic seedling leaves of A. arguta were used as callus-inducing materials. MS medium supplemented with 0.3 mg·L-1 2,4-D and 1.0 mg·L-1 BA was the optimal medium for callus induction of leaves, and medium supplemented with 3 mg·L-1 tZ and 0.5 mg·L-1 IAA was optimal for adventitious shoot regeneration. The best proliferation medium for adventitious buds was MS + 1.0 mg·L-1 BA + 0.3 mg·L-1 NAA. The best rooting medium was 1/2MS + 0.7 mg·L-1 IBA with a 100% rooting rate. For the red flesh hardy kiwi variety ‘Purpurna Saduwa’ (A. arguta var. purpurea), leaves are receptors for Agrobacterium (EHA105)-mediated transformation. The orthogonal experiment was used for the optimization of each genetic transformation parameter and the genetic transformation of the leaves was 21% under optimal conditions. Our study provides technical parameters for applying genetic resources and molecular breeding of kiwifruit with red flesh.

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“…For qualitative traits like nematode and virus resistance in soybean, MAS is immediately applicable, while quantitative traits require further validation and fine mapping to identify tightly linked markers [39]. Pyramiding multiple genes for resistance against various pathogens, nematodes, and insects could significantly boost productivity and ensure more robust oilseed crops [40]. Strategic use of markers in conjunction with conventional breeding is crucial for stabilizing production, particularly under abiotic stresses [41].…”

Section: Marker-assisted Selection In Improvement Of Oilseed Cropsmentioning

confidence: 99%

Advances in Breeding for Oil Quality Enhancement in Indian Mustard (Brassica spp. L.): Achievements, Challenges, and Research Opportunities

Singh,

Prakash

2024

PCBMB

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Major objectives in oil crop improvement are enhancement of seed and oil yield, quality of oil according to its use, i.e. edible or industrial uses, breeding of varieties that fit in different cropping systems and breeding biotic and abiotic stress resistant/tolerant varieties. Despite traditional breeding approaches, including pure line breeding, yielding only modest gains in productivity, recent advancements in mustard breeding have led to significant breakthroughs in both productivity and oil quality. This review discusses the innovative breeding strategies that have contributed to these advancements, with a focus on hybrid development, oil quality enhancement, and biotechnological approaches. To enhance productivity, researchers at the University of Delhi have developed hybrid seed production techniques using transgenic Barnase-barstar systems and cytoplasmic male sterility (CMS) systems. These systems enable large-scale hybrid seed production, with field trials demonstrating significant yield heterosis ranging from 31% to 55% compared to national check varieties. In addition to productivity, improving oil and meal quality has been a key objective. By integrating genes from canola-quality mustard lines, breeders have achieved reductions in erucic acid and glucosinolates, enhancing the health profile and industrial applicability of mustard oil. A high-density linkage map developed using an F1 double haploid mapping population has facilitated the marker-assisted backcross breeding of desirable traits, enabling precise transfer of key quality traits. Transgenic approaches, such as antisense RNA technology, have led to the development of high-oleic, low-linoleic mustard lines with improved fatty acid profiles. These advancements reflect a strategic combination of conventional and biotechnological methods, demonstrating a clear pathway for boosting mustard yields while enhancing oil quality. Molecular markers reported for genetic diversity assessment, mapping and tagging genes/QTLs for different qualitative and quantitative traits and their use in marker-assisted selection have been presented. This progress not only addresses current challenges but also sets the stage for future research aimed at further optimizing productivity, oil quality, and resistance to pests and diseases in mustard cultivation.

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Gene Pyramiding in Transgenic Plant Development: Approaches and Challenges

Cited by 12 publications

References 142 publications

Breeding for durable resistance against biotrophic fungal pathogens using transgenes from wheat

Breeding for durable resistance against biotrophic fungal pathogens using transgenes from wheat

An effective method for establishing a regeneration and genetic transformation system for Actinidia arguta

Advances in Breeding for Oil Quality Enhancement in Indian Mustard (Brassica spp. L.): Achievements, Challenges, and Research Opportunities

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