Gene regulation and signal transduction in the ICE–CBF–COR signaling pathway during cold stress in plants

Wang, Dazhi; Jin, Yanlong; Ding, Xihan; Wang, Wenjia; Zhai, Shanshan; Bai, Li-Ping; Guo, Zhixin

doi:10.1134/s0006297917100030

Cited by 102 publications

(95 citation statements)

References 134 publications

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“…On the other hand, due to differences in cold resistance, geographic distribution and speciation during evolution, the COR genes do not have high homology in different plant species (Wang et al . ). Freezing‐sensitive tomato also has a functional CBF cold‐responsive pathway, but the CBF regulon differs from that of Arabidopsis and appears to be considerably smaller and less diverse in function (Zhang et al .…”

Section: Discussionmentioning

confidence: 97%

An eggplant SmICE1a gene encoding MYC‐type ICE1‐like transcription factor enhances freezing tolerance in transgenic Arabidopsis thaliana

Zhou

et al. 2020

Plant Biol J

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Low temperature is a crucial environmental factor affecting the quality and production of eggplant. Therefore, it is necessary to explore the molecular mechanisms of low temperature response.• We isolated an ICE (inducer of CBF expression) gene from Solanum melongena, named SmICE1a. We then analysed structure, transcriptional activity and expression patterns of SmICE1a. Moreover, we also expressed SmICE1a in Arabidopsis thaliana.• Bioinformatics and expression analysis showed that SmICE1a has a typical S-rich motif, ZIP region, bHLH and ACT-like domain. The gene SmICE1a had transcriptional activity in yeast and was localized to the nucleus following transient expression in tobacco leaves, which suggests that SmICE1a is a transcription factor. A dual-LUC assay revealed that SmICE1a can enhance expression of SmCBF. Overexpression of SmICE1a in Arabidopsis increased freezing tolerance and caused multiple biochemical changes: transgenic lines have higher proline content and lower electrolyte leakage and malondialdehyde than the wild type in cold conditions. The expression of AtCBF and their target genes, AtCOR15A, AtCOR47, AtKIN1 and AtRD29A, were up-regulated in SmICE1a-overexpressing plants under low temperatures.• Based on these results, we suggest that SmICE1a plays an important role in cold response, which may help to understand the cold response mechanism in eggplant and could be used to enhance cold tolerance of eggplant in future.Plant Biology 22 (2020) 450-458

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Section: Discussionmentioning

confidence: 97%

An eggplant SmICE1a gene encoding MYC‐type ICE1‐like transcription factor enhances freezing tolerance in transgenic Arabidopsis thaliana

Zhou

et al. 2020

Plant Biol J

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“…6). In previous studies, increased expression of ICE1 enhanced plant resistance to cold stress [37,52]. Therefore, the increase in ICE1 expression may enhance the adaptability of P. edulis to cold stress.…”

Section: Discussionmentioning

confidence: 80%

Reference gene selection in multiple Passiflora edulis tissues and under cold stress for RT- qPCR analysis

Tu¹,

Fan²,

Li³

et al. 2020

Preprint

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Background: Passiflora edulis is a tropical fruit with high edible and medicinal values that is widely cultivated in southern China. The development of new P. edulis variety with cold resistance and research on the mechanism of cold resistance are the basis for further promoting the cultivation of this species. In a previous study, our laboratory discovered a cold-resistant variety Pingtang 1, which is used as a material for the study of cold resistance mechanism in P. edulis. However, functional genes of P. edulis have not been well studied, especially via relative quantitative analysis. In addition, research on reference genes in P. edulis has not been reported.Results: We used three tools to test the expression stability of 10 candidate reference genes in multiple tissue samples and cold-treated samples of P. edulis. We found that Ts and EF1 showed the highest expression stability in all samples. Further analysis showed that HIS and EF1 were stably expressed in tissue samples, whereas UBQ and EF1 were stably expressed in cold-treated samples. Interestingly, EF1 was stably expressed in not only multiple tissue samples but also cold-treated samples. To validate the selected reference genes, we used the target gene ICE1 and investigated its expression level in cold-treated leaves. Interestingly, the expression of ICE1 increased with increasing cold treatment times.Conclusions: In this study, we successfully selected reference genes from among 10 candidates for P. edulis RT-qPCR data normalization. We selected Ts and EF1 as reference genes for normalization in all samples. HIS and EF1 were ideal for data normalization in tissue samples, whereas UBQ and EF1 were ideal for cold-treated samples. Our work substantially benefits the study of functional genes in P. edulis.

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“…Typically, a cold signal will first lead to damages to the plasma membrane. Next, mitogen-activated protein kinases (MAPKs) and serine/threonine kinases are phosphorylated and enter the nucleus where various transcription factors, enzymes, and other proteins that modulate cellular activities are phosphorylated ( Fujiwara and Denlinger, 2007 ; Wang et al, 2017 ). Our results indicate that free radical stress scavenging enzymes played key roles in white shrimp under cold stress in these experiments, consistent with studies in other species.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Responses Under Cold Stress Reveal Unique Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus vannamei)

Peng

Wei

et al. 2018

Front. Physiol.

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The Pacific white shrimp (Litopenaeus vannamei), one of the most widely cultured shrimp species in the world, often suffers from cold stress. To understand the molecular mechanism of cold tolerance in Pacific white shrimp, we conducted a proteomic analysis on two contrasting shrimp cultivars, namely, cold-tolerant Guihai2 (GH2) and cold-sensitive Guihai1 (GH1), under normal temperature (28°C), under cold stress (16°C), and during recovery to 28°C. In total, 3,349 proteins were identified, among which 2,736 proteins were quantified. Based on gene ontology annotations, differentially expressed proteins largely belonged to biological processes, cellular components, and molecular functions. KEGG pathway annotations indicated that the main changes were observed in the lysosome, ribosomes, and oxidative phosphorylation. Subcellular localization analysis showed a significant increase in proteins present in cytosol, extracellular regions, and mitochondria. Combining enrichment-based clustering analysis and qRT-PCR analysis, we found that glutathione S-transferase, zinc proteinase, m7GpppX diphosphatase, AP2 transcription complex, and zinc-finger transcription factors played a major role in the cold stress response in Pacific white shrimp. Moreover, structure proteins, including different types of lectin and DAPPUDRAFT, were indispensable for cold stress tolerance of the Pacific white shrimp. Results indicate the molecular mechanisms of the Pacific white shrimp in response to cold stress and provide new insight into breeding new cultivars with increased cold tolerance.

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Gene regulation and signal transduction in the ICE–CBF–COR signaling pathway during cold stress in plants

Cited by 102 publications

References 134 publications

An eggplant SmICE1a gene encoding MYC‐type ICE1‐like transcription factor enhances freezing tolerance in transgenic Arabidopsis thaliana

An eggplant SmICE1a gene encoding MYC‐type ICE1‐like transcription factor enhances freezing tolerance in transgenic Arabidopsis thaliana

Reference gene selection in multiple Passiflora edulis tissues and under cold stress for RT- qPCR analysis

Proteomic Responses Under Cold Stress Reveal Unique Cold Tolerance Mechanisms in the Pacific White Shrimp (Litopenaeus vannamei)

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