Gene Regulation of BMP Ligands in Drosophila

Stultz, Brian G.; Hursh, Deborah A.

doi:10.1007/978-1-4939-8904-1_7

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…Several proteins have been investigated for interaction with BMPs in insects and mammals, including decapentaplegic protein (Dpp), Screw (Scw), short gastrulation (SOG or chordin), and twisted gastrulation (Tsg) (Brazil et al, 2015; Peterson & O'Connor, 2012; Stultz & Hursh, 2019). The heterodimer of BMPs is required for their downstream activities.…”

Section: Discussionmentioning

confidence: 99%

“…The transforming growth factor‐beta (TGF‐β) is one of the hormones that regulate development in many organisms. In arthropods, a member of the TGF‐β family kwon as glass bottom boat (Gbb), an orthologue of bone morphogenetic protein (BMP) isoforms 5/6/7/8, plays a role in developmental regulation (Peterson & O'Connor, 2012; Stultz & Hursh, 2019). In high‐fat induced fruit flies, Gbb controls triglyceride and glucose levels (Hong et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Stimulatory effect of peritrophin on vitellogenesis in female banana shrimp, Fenneropenaeus merguiensis

Sathapondecha

Nonsung

Chotigeat

2022

Aquaculture Research

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Glass bottom boat (Gbb) is a member of the transforming growth factor β family that plays several physiological roles in arthropods, including in reproduction. Recently, Fenneropenaeus merguiensis's Gbb (FmGbb) was demonstrated to play a stimulatory role in vitellogenesis of the banana shrimp, but potential partners of FmGbb have not been identified. Therefore, this study aimed to identify proteins that interact with FmGbb and characterize their gonad‐regulating function. A yeast two‐hybrid assay revealed three potential candidates: peritrophin (FmPT), polehole‐like protein (FmPhl), and an uncharacterized protein. Expressions of FmPT and FmPhl were found mainly in the ovaries and significantly increased in the developing stages. However, in a GST‐pull down assay, there was no protein–protein interaction between soluble recombinant glutathione‐S transferase‐fused FmPT (GST‐rPT) protein and the recombinant FmGbb protein (His‐TF‐rgbb). The vitellogenesis stimulatory role of FmPT was then investigated both in vitro and in vivo. Ovarian explants treated with GST‐rPT showed no significant change of vitellogenin (Vg) expression, but the injection of GST‐rPT in ovaries and hepatopancreas of previtellogenic shrimp did induc Vg expression, suggesting a vitellogenesis stimulating activity of FmPT. Our findings show that FmPT has a novel role in vitellogenesis stimulation and has the potential to be developed as a gonad‐stimulating bioproduct for use in shrimp aquaculture.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stimulatory effect of peritrophin on vitellogenesis in female banana shrimp, Fenneropenaeus merguiensis

Sathapondecha

Nonsung

Chotigeat

2022

Aquaculture Research

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Bone Morphogenetic Protein (BMP) signaling regulates germline stem cell self-renewal in the newly formed Drosophila testis stem cell niche

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Fidler

Jones

et al. 2022

Preprint

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Bone morphogenetic proteins (BMPs) are a group of multifunctional cytokines and metabologens highly conserved in the transforming growth factor-β (TGF-β) superfamily. BMPs have an established role in controlling cell fate and tissue morphogenesis in a diverse range of organisms and are known to maintain stem cells in adult Drosophila testes. Additional studies in embryonic and larval testes suggest BMP regulates germ cell behavior. However, the roles of BMP signaling in controlling testis stem cell formation have yet to be directly examined. Here, we explore the pattern of BMP activation during embryonic testis niche morphogenesis as well as niche maturation in larval testes. We also assess the impact of altered BMP signaling on these stages of development. Our data suggest that BMP signaling is critical for promoting germ cell identity in primordial germ cells during embryonic niche formation. During niche maturation, we also find that that BMP signaling is both necessary and sufficient for maintenance of a self-renewing germline stem cell (GSC) population, and that newly formed cyst stem cells (CySCs) are a source of BMP activating ligand. As development progresses, however, BMP activation is no longer sufficient to alter GSC self-renewal. Collectively, our work promotes a more thorough understanding of BMP as a key mechanism controlling stem cell development in Drosophila testes that has implications for the development of other organ systems.

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Gbb Regulates Blood Cell Proliferation and Differentiation through JNK and EGFR Signaling Pathways in the Drosophila Lymph Gland

Zhang

Wang

et al. 2023

Cells

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The Drosophila lymph gland is an ideal model for studying hematopoiesis, and unraveling the mechanisms of Drosophila hematopoiesis can improve our understanding of the pathogenesis of human hematopoietic malignancies. Bone morphogenetic protein (BMP) signaling is involved in a variety of biological processes and is highly conserved between Drosophila and mammals. Decapentaplegic (Dpp)/BMP signaling is known to limit posterior signaling center (PSC) cell proliferation by repressing the protooncogene dmyc. However, the role of two other TGF-β family ligands, Glass bottom boat (Gbb) and Screw (Scw), in Drosophila hematopoiesis is currently largely unknown. Here, we showed that the loss of Gbb in the cortical zone (CZ) induced lamellocyte differentiation by overactivation of the EGFR and JNK pathways and caused excessive differentiation of plasmatocytes, mainly by the hyperactivation of EGFR. Furthermore, we found that Gbb was also required for preventing the hyperproliferation of the lymph glands by inhibiting the overactivation of the Epidermal Growth Factor Receptor (EGFR) and c-Jun N-terminal Kinase (JNK) pathways. These results further advance our understanding of the roles of Gbb protein and the BMP signaling in Drosophila hematopoiesis and the regulatory relationship between the BMP, EGFR, and JNK pathways in the proliferation and differentiation of lymph gland hemocytes.

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Gene Regulation of BMP Ligands in Drosophila

Cited by 3 publications

References 47 publications

Stimulatory effect of peritrophin on vitellogenesis in female banana shrimp, Fenneropenaeus merguiensis

Stimulatory effect of peritrophin on vitellogenesis in female banana shrimp, Fenneropenaeus merguiensis

Bone Morphogenetic Protein (BMP) signaling regulates germline stem cell self-renewal in the newly formed Drosophila testis stem cell niche

Gbb Regulates Blood Cell Proliferation and Differentiation through JNK and EGFR Signaling Pathways in the Drosophila Lymph Gland

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