Gene Response to Stress in the Asian Citrus Psyllid (Hemiptera: Psyllidae)

Marutani-Hert, Mizuri; Hunter, Wayne B.; Hall, David G.

doi:10.1653/024.093.0408

Cited by 21 publications

(7 citation statements)

References 20 publications

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“…As such, the observation of temperatures above 40 °C significantly decreasing D. citri flight capacity was not unexpected. Previous studies have showed high temperatures as detrimental for different D. citri life traits such as survivorship, oviposition, longevity or hatching [ 41 , 42 , 46 , 47 ]. Exposure to temperatures above 40 °C induces the transcription of heat shock proteins and may cause ATPase and protein denaturation [ 40 , 41 ] which are critical for survival.…”

Section: Discussionmentioning

confidence: 99%

High Temperatures Decrease the Flight Capacity of Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Antolínez

Moyneur

Martini

et al. 2021

Insects

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Diaphorina citri Kuwayama (Hemiptera: Liviidae), commonly known as Asian citrus psyllid (ACP), is an invasive insect pest and the vector of the bacterium causing Huanglongbing (HLB), a lethal disease of citrus. In the United States, ACP has been established in all citrus-producing zones, all of which have different environmental conditions. The spread of ACP and, more importantly, HLB, has progressed differently depending on the state, with more rapid spread in Florida and Texas than in California. Climatic variations between the regions are likely a strong factor in the difference in the rate of spread. Despite this, it is unknown how the flight capacity of D. citri is influenced by high temperatures (>30 °C) and subsequently, low humidity experienced in California but not in Texas or Florida. In this study, by using a custom-made, temperature-controlled flight mill arena, we assessed the effect of high temperatures on the flight capacity and flight propensity of D. citri under low (20–40%) and high (76–90%) relative humidity conditions. We found that temperature and humidity influence the propensity to engage in short or long-distance flight events. Psyllids exposed to temperatures above 43 °C only performed short flights (˂60 s), and a high relative humidity significantly decrease the proportion of long flights (≥60 s) at 26 and 40 °C. The flight capacity for insects who engaged in short and long flights was significantly affected by temperature but not by humidity. For long flyers, temperature (in the 26–43 °C range) was negatively correlated with distance flown and flight duration. The most favorable temperature for long dispersion was 26 °C, with suboptimal temperatures in the range of 32–37 °C and the least favorable temperatures at 40 and 43 °C. In conclusion, D. citri is able to fly in a broad range of temperatures and efficiently fly in high and low humidity. However, temperatures above 40 °C, similar to those experienced in semi-arid environments like Southern California or Arizona, are detrimental for its flight capacity.

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Section: Discussionmentioning

confidence: 99%

High Temperatures Decrease the Flight Capacity of Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Antolínez

Moyneur

Martini

et al. 2021

Insects

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“…A novel approach being investigated to control ACP is using RNA interference (RNAi) to disrupt or regulate certain genes (Marutani-Hert et al, 2010;Wuriyanghan et al, 2011). RNAi is a natural gene regulatory and immune response of eukaryotic cells and is activated by double-stranded (ds) RNA.…”

Section: Molecular Research For Asian Citrus Psyllid/ Huanglongbing Cmentioning

confidence: 99%

Asian citrus psyllid, Diaphorina citri, vector of citrus huanglongbing disease

Hall

Richardson

Ammar

et al. 2012

Entomologia Exp Applicata

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368

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The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is an important pest of citrus because it transmits phloem-limited bacteria [Candidatus Liberibacter spp., notably Ca. L. asiaticus (LAS)] associated with huanglongbing (HLB; citrus greening disease), currently considered the world's most serious disease of citrus. Asian citrus psyllid transmits LAS in a persistent manner and, although the rate of LAS transmission by ACP individuals usually is low, HLB can spread rapidly in a citrus grove and the geographic range of the disease is expanding, threatening citrus industries in new areas. Intensive chemical control of ACP is the primary management strategy currently advocated for HLB, but this strategy is costly, unsustainable, and generally ineffective. The scientific community is searching aggressively for solutions to HLB on many fronts, but it could still be years before solutions are found and implemented. Plant resistance to LAS is one area of research being pursued, whereby traits that confer resistance are identified and incorporated into citrus germplasm through conventional or transgenic methods. It remains to be seen if a solution to HLB can be found that specifically targets ACP, but research on ACP has been stepped up in a number of areas, notably on ACP-LAS-plant interactions, on host plant resistance to ACP, and on molecular methods of silencing ACP genes to induce mortality or to block its ability to transmit HLB-causing bacteria. Advancements in these and other research areas may depend greatly on a better understanding of basic ACP biology and vector-pathogen-host plant interactions at the molecular, cellular, and community levels. Here, we present an updated review of ACP and HLB with an emphasis on the problem in Florida.

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“…To develop molecular methods that exploit current gene-targeting technologies, detailed genetic and genomic knowledge, including a high-quality official gene set (OGS), is required ( 16 , 17 ). Early efforts focused on D. citri transcript expression ( 3 , 18–20 ), analysis of the full transcriptome ( 21 , 22 ) and, more recently, analysis of the D. citri proteome ( 16 ). Arp et al ( 23 ) performed a BLAST-based inventory of NCBI-predicted immune genes in D. citri (v100, see Materials and methods).…”

Section: Introductionmentioning

confidence: 99%

Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community

et al. 2017

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The Asian citrus psyllid (Diaphorina citri Kuwayama) is the insect vector of the bacterium Candidatus Liberibacter asiaticus (CLas), the pathogen associated with citrus Huanglongbing (HLB, citrus greening). HLB threatens citrus production worldwide. Suppression or reduction of the insect vector using chemical insecticides has been the primary method to inhibit the spread of citrus greening disease. Accurate structural and functional annotation of the Asian citrus psyllid genome, as well as a clear understanding of the interactions between the insect and CLas, are required for development of new molecular-based HLB control methods. A draft assembly of the D. citri genome has been generated and annotated with automated pipelines. However, knowledge transfer from well-curated reference genomes such as that of Drosophila melanogaster to newly sequenced ones is challenging due to the complexity and diversity of insect genomes. To identify and improve gene models as potential targets for pest control, we manually curated several gene families with a focus on genes that have key functional roles in D. citri biology and CLas interactions. This community effort produced 530 manually curated gene models across developmental, physiological, RNAi regulatory and immunity-related pathways. As previously shown in the pea aphid, RNAi machinery genes putatively involved in the microRNA pathway have been specifically duplicated. A comprehensive transcriptome enabled us to identify a number of gene families that are either missing or misassembled in the draft genome. In order to develop biocuration as a training experience, we included undergraduate and graduate students from multiple institutions, as well as experienced annotators from the insect genomics research community. The resulting gene set (OGS v1.0) combines both automatically predicted and manually curated gene models. Database URL: https://citrusgreening.org/

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Gene Response to Stress in the Asian Citrus Psyllid (Hemiptera: Psyllidae)

Cited by 21 publications

References 20 publications

High Temperatures Decrease the Flight Capacity of Diaphorina citri Kuwayama (Hemiptera: Liviidae)

High Temperatures Decrease the Flight Capacity of Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Asian citrus psyllid, Diaphorina citri, vector of citrus huanglongbing disease

Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community

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