Gene sequence analysis of an opportunistic wheat pathogen, an isolate of &lt;i&gt;Fusarium proliferatum&lt;/i&gt;

Kwon, Sunil; Dohlen, Carol D. von; Anderson, Anne J.

doi:10.1139/cjb-79-9-1115

Cited by 14 publications

(10 citation statements)

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“…Although identification of Fusarium species dependent on morphological observations alone may not be sufficient, there are several critical features that provide useful information in distinguishing species with similar appearances to some extent (Leslie and Summerell, ). Generally, the morphological characteristics of the Fusarium isolates in this study were comparable to the previous descriptions (Kwon et al , ; Leslie and Summerell, ; Nirmaladevi et al , ). Morphologically, F. concentricum is similar to F. mangiferae .…”

Section: Discussionsupporting

confidence: 91%

Morphology, phylogeny and pathogenicity of Fusarium species from Sansevieria trifasciata in Malaysia

2020

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Leaf blight is a common disease affecting Sansevieria trifasciata in many countries, including Malaysia. In the present study, Fusarium isolates were consistently recovered from the diseased leaves collected from various locations throughout the country. Based on morphology and multigene phylogenetic analysis using mitochondrial small subunit (mtSSU), intergenic spacer region (IGS) and translation elongation factor 1‐α (TEF1‐α) gene sequences, seven Fusarium species were identified, with F. oxysporum being the most prevalent (67.6%) among 34 isolates. Pathogenicity tests resulted in the discovery of pathogenic isolates that belonged to F. oxysporum, F. proliferatum, and F. pseudocircinatum, whereas all isolates of F. brachygibbosum, F. concentricum, F. mangiferae, and F. solani were nonpathogenic. The results suggest that several Fusarium species are accountable for causing disease on S. trifasciata in Malaysia.

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Section: Discussionsupporting

confidence: 91%

Morphology, phylogeny and pathogenicity of Fusarium species from Sansevieria trifasciata in Malaysia

2020

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“…The group Hypocreales (relative abundance: 2.2%) contained only one specific isolate, F37, which had a high similarity to Fusarium proliferatum strain NRRL 31071, an endophytic fungus of wheat (Kwon et al 2001). This is another group that was frequently isolated from other sponge samples Baker et al 2009).…”

Section: Diversity Of Culturable Fungi Associated With Haliclona Simumentioning

confidence: 99%

Phylogenetic diversity of culturable fungi associated with two marine sponges: Haliclona simulans and Gelliodes carnosa, collected from the Hainan Island coastal waters of the South China Sea

Liu

Zhu

et al. 2010

Fungal Diversity

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The diversity and biological activities of fungi associated with the two sponges, Haliclona simulans and Gelliodes carnosa, were investigated using a culturedependent method followed by analysis of the fungal rDNA-ITS sequences. The two sponges were collected from the coastal waters of Lingshui Bay of Hainan Island in the South China Sea. A total of 37 independent fungal isolates corresponding to 30 different species were obtained from the two sponges. Nearly two thirds of the strains (n=24, 64.9%) had close affiliations (identity (ID) or similarity≥98%) with their best matches in GenBank. Another one third of the isolates (n=13, 35.1%) were distantly related to their closest relatives (ID<98%), implying that these species are possibly different from those previously reported. The two sponges possessed similar fungal diversities. Haliclona simulans harbored a mainly different fungal consortium as compared with that of the same sponge species collected from Irish coastal waters, suggesting that the fungal diversity associated with the sponges is more dependent on the surrounding environment than on the sponge species. Biological activities of the fungal culture extracts were tested against the human tumor cell lines, mainly, a human lung carcinoma cell line (A-549), a human liver carcinoma cell line (Bel-7402), and a human colon carcinoma cell line (HCT-8), and against the Gram positive bacterium Bacillus subtilis. A relatively high proportion of positive results were obtained in this study, demonstrating that fungi isolated from sponges could be a rich source of new biologically active natural products.

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“…Deduced amino acid sequences of partial rpo1 and rpo2 have 52% identity with lac2 of Gaeumannomyces graminis var. tritici Walker (Litvintseva and Henson 2002) and 60% identity with laccase of Fusarium proliferatum (Matsushima) Nirenberg (Kwon et al 2001), respectively. Therefore, the two genes were not alleles.…”

Section: Resultsmentioning

confidence: 99%

“…RPO1, R. necatrix phenol oxidase 1; GLCC1, Gaeumannomyces graminis var. tritici laccase (Litvintseva and Henson 2002); RPO2, R. necatrix phenol oxidase 2; FLCC, Fusarium proliferatum laccase (Kwon et al 2001); RCH1, R. necatrix chitinase 1; RCH2, R. necatrix chitinase 2; CHIB and CHIC, Aspergillus nidulans chitinase B and C, respectively (accession numbers in DDBJ are D87063 and AF314225, respectively); RXY1, R. necatrix xylanase 1; CGXB, Chaetomium gracile xylanase B (Yoshino et al 1995) …”

Section: Rflp Analysismentioning

confidence: 99%

Heterothallic life cycle in the white root rot fungus Rosellinia necatrix

et al. 2003

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To prepare homologous DNA fragments as restriction fragment length polymorphism (RFLP) markers, the genes encoding phenol oxidase, chitinase, and xylanase were amplified from genomic DNA of Rosellinia necatrix strains. RFLP analysis using the amplified DNA fragments as probe was carried out, with segregation of the markers among two sets of F 1 progenies isolated from an independent perithecium. RFLP was frequently found using rpo1 as the RFLP marker among strains of R. necatrix, which was isolated from single ascospores and the circumference of the perithecium. In each set, RFLPs of some F 1 progenies were different from that of the parent strain. Random amplified polymorphic DNA (RAPD) also revealed that several strains, which were of different genotypes from the parent strain, were contained in the single ascospore culture isolated from the same perithecium. From these results, it is suggested that another strain, which was genetically different, was required for mating and development of the ascus in R. necatrix. Therefore, the life cycle in R. necatrix was presumed to be heterothallism. This is the first report about a heterothallic life cycle in R. necatrix.

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Gene sequence analysis of an opportunistic wheat pathogen, an isolate of <i>Fusarium proliferatum</i>

Cited by 14 publications

References 0 publications

Morphology, phylogeny and pathogenicity of Fusarium species from Sansevieria trifasciata in Malaysia

Morphology, phylogeny and pathogenicity of Fusarium species from Sansevieria trifasciata in Malaysia

Phylogenetic diversity of culturable fungi associated with two marine sponges: Haliclona simulans and Gelliodes carnosa, collected from the Hainan Island coastal waters of the South China Sea

Heterothallic life cycle in the white root rot fungus Rosellinia necatrix

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