Gene silencing in the aedine cell lines C6/36 and U4.4 using long double-stranded RNA
Bodunrin Omokungbe,
Alejandra Centurión,
Sabrina Stiehler
et al.
Abstract:Background
RNA interference (RNAi) is a target-specific gene silencing method that can be used to determine gene functions and investigate host–pathogen interactions, as well as facilitating the development of ecofriendly pesticides. Commercially available transfection reagents (TRs) can improve the efficacy of RNAi. However, we currently lack a product and protocol for the transfection of insect cell lines with long double-stranded RNA (dsRNA).
Methods
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