Gene-specific universal mammalian sequence-tagged sites: Application to the canine genome

Evaluation of a short interspersed nucleotide element in the 3' untranslated region of the defective dystrophin gene of dogs with muscular dystrophy

“…There appears to be little sequence conservation between these repeats, although there is evidence of a pattern of polyadenylation-rich motifs (Table 2). [16][17][18][19][20][21][22][23][24][25]…”

“…27 In addition, others have identified this SINE in a number of other carnivores including harbor seals (Phoca vitulina concolour), 28 red foxes (Vulpes vulpes), 29 giant and red pandas (Ailuropoda melanoleuca and Ailurus fulgens, respectively), 19 and mink (Mustela vison). 28,30 Insertion of these elements is believed to require reverse transcription of class-III genes encoding small 20 TAAAATAAATTTTA Canis familiaris cGMP-gated channel a subunit (intron 2) 16 TTAAAATCAATTAATT Canis familiaris tyrosinase-related protein 17 TAAACTACTCCAG Canis familiaris tRNA-derived SINE 21 AAAAAAGAAAT(+N)CATTTGT Canis familiaris blood clotting Factor IX 3' UTR 18 AAAGG(C/T)ATGAGTCA Canis familiaris 5' to pancreatic colipase gene 22 AGAACAGATAACTTG Canis familiaris interferon ω 5' UTR 23 AAGAAAG Canis familiaris retinoblastoma gene 5' UTR 24,25 AAAAGATTA Potos flavus transthyretin intron 1 19 AAAG(G/A)ATAGATCCTT(C/T)CAG Imperfect repeats are indicated; differences are shown in parentheses.…”

First endangered black-footed ferrets,Mustela nigripes, cloned for genetic rescue

“…Between sample extractions, the area and pipettes were UV-radiated for 24 hours. Gene regions were amplified via PCR with a 50 µl volume reaction using Qiagen Taq PCR Core Kit (Qiagen, Hilden, Germany) BRCA-1 and GHR forward and reverse primers 18,19 at 0.5µM final concentration each, 200µM each dNTP, 1.25U Taq, 2.5 mM MgCl2, 3µl gDNA (100-1000 ng/µl). BRCA-1 PCR amplification conditions were 94°C 2 mins denaturation followed by 30 cycles: 94°C 30 s., 55.4°C 45 s., 72°C 45 s. and final at 72°C for 7 min.…”

“…The nuclear DNA sequences amplified were Exon 9 of the Breast and Ovarian Cancer Susceptibility gene (BRCA-1) 18 , and an intron-exon region of the Growth Hormone Receptor gene (GHR) 19 . These sequences are diagnostic between species.…”

Phylogenetic relationships between different raccoon dog (Nyctereutes procyonoides) populations based on four nuclear and Y genes

“…We selected four nuclear genes: CHRNA1 (Cholinergic receptor, nicotinic, α polypeptide 1 precursor), VTN (Vitronectin), TRSP (Selenocysteine tRNA), and WT1 (Wilms tumor 1) to compare genetic variation among different raccoon dog populations. They have been used for the phylogenetic study of mammals including Canidae (Venta et al 1996;Bardeleben et al 2005;Koepfli et al 2006). A zinc-finger-containing gene located on the Y chromosome (ZFY) shows male-driven evolution (Nakagome et al 2008).…”