Gene structure and cDNA sequence of 2-Cys peroxiredoxin in the harmful algal bloom species <i>Chattonella marina</i> and its gene transcription under different light intensities

Mukai, Koki; Teramoto, Ayano; Qiu, Xuchun; Shimasaki, Yohei; Kato-Unoki, Yoko; Lee, Jae Man; Mizoguchi, Naohiro; Khanam, Mst Ruhina Margia; Satone, Hina; Tatsuke, Tsuneyuki; Kusakabe, Takahiro; Oshima, Yuji

doi:10.1080/09670262.2017.1346206

Cited by 8 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To elucidate the mechanism responsible for the production of 2-CysPrx, we determined the structure of the 2-CysPrx gene and the predicted amino acid sequence using the rapid amplification of cDNA ends (RACE) method [ 51 ]. The open reading frame of the 2-CysPrx gene was 585 base pairs (bp) long and encoded a protein consisting of 195 amino acids.…”

Section: Structure Of the 2-cysprx Gene In Chattonella Mmentioning

confidence: 99%

“…In addition, inverse polymerase chain reaction (PCR) analysis revealed possible TATA and GGA motifs upstream of the 2-CysPrx gene that were recognized by nuclear-encoded plastid RNA polymerase (NEP) as well as a possible −10 and −35 box recognized by plastid-encoded plastid RNA polymerase (PEP). These results suggested the possibility that the C. marina 2-CysPrx gene is found in the chloroplast, and its transcription can be regulated by both NEP and PEP [ 51 ].…”

Section: Structure Of the 2-cysprx Gene In Chattonella Mmentioning

confidence: 99%

“…Antioxidant enzymes are essential proteins that maintain algal cell proliferation potential by protecting against oxidative stress induced by photosynthetic processes. We, therefore, investigated the induction of the genes that code for the production of 2-CysPrx and five other antioxidant enzymes (Cu Zn superoxide dismutase (Cu/Zn-SOD), glutathione peroxidase (GPX), catalase (CAT), ascorbate peroxidase (APX), and thioredoxin (TRX)) under different light, oxidative stress, and temperature (2-CysPrx only) conditions using quantitative PCR [ 51 , 57 , 58 ]. In dark-acclimated cells, expression levels of all antioxidant enzymes except APX were significantly increased by light irradiation (100 μmol photons m −2 s −1 ), and these expression levels decreased within 24 h, whereas the expression of the 2-CysPrx gene remained high for 24 h [ 57 ].…”

Section: Induction Of Gene Expression For the Production Of Antioxmentioning

confidence: 99%

“…However, in C. marina cells acclimated to 100 μmol photons m −2 s −1 , only the levels of expression of the 2-CysPrx gene were significantly ( p < 0.05) increased by strong light irradiation (1000 μmol photons m −2 s −1 ) ( Figure 4 ). In that experiment, both the H 2 O 2 concentration and scavenging activity also increased [ 51 ]. Extracellular exposure to H 2 O 2 at 21.5 μM ( Figure 5 , manuscript in preparation) induced expression of the genes that code for the production of six antioxidant enzymes, and 100 μM of H 2 O 2 [ 59 ] also induced expression of the 2-CysPrx gene.…”

Section: Induction Of Gene Expression For the Production Of Antioxmentioning

confidence: 99%

“…Sixty milliliter cell suspensions in plastic flasks were then cultured at 20, 110, or 520 µmol photons m –2 s –1 of continuous irradiance by LED bulbs (low, moderate, or high irradiance, respectively) for 13 h (N = 4). During exposure, an 8.5 mL cell suspension was sampled from each flask at 1, 7, and 13 h. Soon after sampling, a portion of each cell suspension was assayed for cell counts, measurement of concentrations of O 2 − [ 70 ] and H 2 O 2 , and evaluation of photosynthetic activity [ 51 ]. The remaining cells were pelletized by centrifugation at 1800 × g for 10 min, and the cell pellet was stored at −80 °C until LPO analysis (Lipid Hydroperoxide Assay Kit, Cayman Chemical, Ann Arbor, MI, USA).…”

Section: Effect Of 2-cysprx Protein Levels On Survival and Productmentioning

confidence: 99%

See 4 more Smart Citations

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

et al. 2021

Self Cite

View full text Add to dashboard Cite

Peroxiredoxin (Prx) is a relatively recently discovered antioxidant enzyme family that scavenges peroxides and is known to be present in organisms from biological taxa ranging from bacteria to multicellular eukaryotes, including photosynthetic organisms. Although there have been many studies of the Prx family in higher plants, green algae, and cyanobacteria, few studies have concerned raphidophytes and dinoflagellates, which are among the eukaryotic algae that cause harmful algal blooms (HABs). In our proteomic study using 2-D electrophoresis, we found a highly expressed 2-Cys peroxiredoxin (2-CysPrx) in the raphidophyte Chattonella marina var. antiqua, a species that induces mass mortality of aquacultured fish. The abundance of the C. marina 2-CysPrx enzyme was highest in the exponential growth phase, during which photosynthetic activity was high, and it then decreased by about a factor of two during the late stationary growth phase. This pattern suggested that 2-CysPrx is a key enzyme involved in the maintenance of high photosynthesis activity. In addition, the fact that the depression of photosynthesis by excessively high irradiance was more severe in the 2-CysPrx low-expression strain (wild type) than in the normal-expression strain (wild type) of C. marina suggested that 2-CysPrx played a critical role in protecting the cell from oxidative stress caused by exposure to excessively high irradiance. In the field of HAB research, estimates of growth potential have been desired to predict the population dynamics of HABs for mitigating damage to fisheries. Therefore, omics approaches have recently begun to be applied to elucidate the physiology of the growth of HAB species. In this review, we describe the progress we have made using a molecular physiological approach to identify the roles of 2-CysPrx and other antioxidant enzymes in mitigating environmental stress associated with strong light and high temperatures and resultant oxidative stress. We also describe results of a survey of expressed Prx genes and their growth-phase-dependent behavior in C. marina using RNA-seq analysis. Finally, we speculate about the function of these genes and the ecological significance of 2-CysPrx, such as its involvement in circadian rhythms and the toxicity of C. marina to fish.

show abstract

Section: Structure Of the 2-cysprx Gene In Chattonella Mmentioning

confidence: 99%

Section: Structure Of the 2-cysprx Gene In Chattonella Mmentioning

confidence: 99%

Section: Induction Of Gene Expression For the Production Of Antioxmentioning

confidence: 99%

Section: Induction Of Gene Expression For the Production Of Antioxmentioning

confidence: 99%

Section: Effect Of 2-cysprx Protein Levels On Survival and Productmentioning

confidence: 99%

See 3 more Smart Citations

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.

Feng

Cai

Chen

et al. 2020

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Time-series responses in photosynthetic activity, 2-cysteine peroxiredoxin gene expression, and proteomics of Chattonella marina var. antiqua under different oxidative stress

et al. 2020

View full text Add to dashboard Cite

Gene structure and cDNA sequence of 2-Cys peroxiredoxin in the harmful algal bloom species Chattonella marina and its gene transcription under different light intensities

Cited by 8 publications

References 28 publications

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

Effects of an exopolysaccharide from Lactococcus lactis Z-2 on innate immune response, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio L.

Time-series responses in photosynthetic activity, 2-cysteine peroxiredoxin gene expression, and proteomics of Chattonella marina var. antiqua under different oxidative stress

Contact Info

Product

Resources

About