Gene structure and differential modulation of multiple rockbream (Oplegnathus fasciatus) hepcidin isoforms resulting from different biological stimulations

Cho, Young S.; Lee, Sang Yup; Kim, Ki H.; Kim, Sung K.; Kim, Dong S.; Nam, Yoon Kwon

doi:10.1016/j.dci.2008.07.009

Cited by 58 publications

(58 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of this study are consistent with many previous reports on the variable basal levels of sod1 transcripts across different tissue types Nam et al, 2006). Furthermore, our finding of robust expression of sod1 transcripts in the liver is consistent with the presence of multiple binding motifs for liver-enriched transcription factors such as C/EBP and hepatocyte nuclear factors (HNFs), in the 5'-upstream regulatory region of hmsod1 [see Cho et al (2009a)]. The important role of C/EBP in the liver-predominant expression of rat sod1 has also been reported previously (Kim et al, 1997).…”

Section: Tissue Distribution and Basal Mrna Expressionsupporting

confidence: 82%

See 1 more Smart Citation

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Lee¹,

Kim²,

Bang³

et al. 2011

Fisheries and aquatic sciences

View full text Add to dashboard Cite

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

show abstract

Section: Tissue Distribution and Basal Mrna Expressionsupporting

confidence: 82%

“…Preparation of bacterial culture was carried out as described previously (Cho et al, 2009a). A freshly grown E. tarda (5×10 3 cells) was resuspended in 100 μL of 0.85% NaCl solution.…”

Section: Bacterial Challenge and Real-time Rt-pcr Assaysmentioning

confidence: 99%

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Lee¹,

Kim²,

Bang³

et al. 2011

Fisheries and aquatic sciences

View full text Add to dashboard Cite

show abstract

“…As such, and unlike mammals, in which a single molecule is performing a dual function as an antimicrobial peptide and iron-regulatory hormone, it is possible that the existence of multiple hepcidins in fish led to a subfunctionalization. As in mammals, the highest expression levels of hepcidin in fish are found in the liver (16)(17)(18)(19)(20)(21)(22)(23)(24)(25). However, fish seem to have a wide tissue distribution of the different hepcidin isoforms, suggesting that each hepcidin isoform may assume a different biological role, depending on the tissue in which it is expressed.…”

mentioning

confidence: 95%

“…Fish hepcidins have been shown to respond to several infectious stimuli, such as Gram-negative and Gram-positive bacteria (7,16,20,21,(23)(24)(25)(26)(27)(28)(29), virus (16,24), parasites (30), and LPS (17,18,22,(31)(32)(33), in some cases with different isoforms presenting differential expressions (16-18, 24, 29, 33). Other studies have examined the effects of iron overload in hepcidin (16-18, 21, 24, 28, 32), showing its involvement in the control of iron levels in fish.…”

mentioning

confidence: 99%

Multiple Hepcidins in a Teleost Fish, Dicentrarchus labrax: Different Hepcidins for Different Roles

Neves

Caldas

Vieira

et al. 2015

The Journal of Immunology

View full text Add to dashboard Cite

Teleost fish rely heavily on their innate immunity for an adequate response against pathogens and environmental challenges, with the production of antimicrobial peptides being one of their first lines of defense. Among those is hepcidin, a small cysteine-rich antimicrobial peptide that is also the key regulator of iron metabolism. Although most mammals possess a single hepcidin gene, with a dual role in both iron metabolism regulation and antimicrobial response, many teleost fish present multiple copies of hepcidin, most likely because of genome duplications and positive Darwinian selection, suggesting that different hepcidins may perform different functions. To study the roles of hepcidin in teleost fish, we have isolated and characterized several genes in the European sea bass (Dicentrarchus labrax) and evaluated variations in their expression levels in response to different experimental conditions. Although several hepcidin genes were found, after phylogenetic analysis they could be clustered in two groups: hamp1-like, with a single isoform similar to mammalian hepcidins, and hamp2-like, with several isoforms. Under experimental conditions, hamp1 was upregulated in response to iron overload and infection and downregulated during anemia and hypoxic conditions. Hamp2 did not respond to either iron overload or anemia but was highly upregulated during infection and hypoxia. In addition, Hamp2 synthetic peptides exhibited a clear antimicrobial activity against several bacterial strains in vitro. In conclusion, teleost fish that present two hepcidin types show a degree of subfunctionalization of its functions, with hamp1 more involved in the regulation of iron metabolism and hamp2 mostly performing an antimicrobial role.

show abstract

“…Freshly grown E. tarda (strain FSW910410; Cho et al 2009)) was washed twice with phosphate buffered saline (PBS, pH 6.8) and suspended in PBS. Individuals (average BW = 12.1 ± 3.4 g; n = 40 in total) were intraperitoneally injected with E. tarda (1 × 10 6 cells/g body weight) .…”

Section: In Vivo Bacterial Challengementioning

confidence: 99%

Gene structure and expression characteristics of liver-expressed antimicrobial peptide-2 isoforms in mud loach (Misgurnus mizolepis, Cypriniformes)

Lee

Nam

2017

Fish Aquatic Sci

View full text Add to dashboard Cite

Background: Liver-expressed antimicrobial peptide-2 (LEAP-2) is an important component of innate immune system in teleosts. In order to understand isoform-specific involvement and regulation of LEAP-2 genes in mud loach (Misgurnus mizolepis, Cypriniformes), a commercially important food fish, this study was aimed to characterize gene structure and expression characteristics of two paralog LEAP-2 isoforms. Results: Mud loach LEAP-2 isoforms (LEAP-2A and LEAP-2B) showed conserved features in the core structure of mature peptides characterized by four Cys residues to form two disulfide bonds. The two paralog isoforms represented a tripartite genomic organization, known as a common structure of vertebrate LEAP-2 genes. Bioinformatic analysis predicted various transcription factor binding motifs in the 5′-flanking regions of mud loach LEAP-2 genes with regard to development and immune response. Mud loach LEAP-2A and LEAP-2B isoforms exhibited different tissue expression patterns and were developmentally regulated. Both isoforms are rapidly modulated toward upregulation during bacterial challenge in an isoform and/or tissue-dependent fashion.

show abstract

Gene structure and differential modulation of multiple rockbream (Oplegnathus fasciatus) hepcidin isoforms resulting from different biological stimulations

Cited by 58 publications

References 42 publications

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Multiple Hepcidins in a Teleost Fish, Dicentrarchus labrax: Different Hepcidins for Different Roles

Gene structure and expression characteristics of liver-expressed antimicrobial peptide-2 isoforms in mud loach (Misgurnus mizolepis, Cypriniformes)

Contact Info

Product

Resources

About