Gene-targeted molecular phylogeny, phytochemical profiling, and antioxidant activity of nine species belonging to family Cactaceae

Abouseadaa, Heba H.; Atia, Mohamed A. M.; Younis, Inas Y.; Issa, Marwa Y.; Ashour, Haraz A.; Saleh, İbrahim; Osman, Gamal; Arif, Ibrahim A.; Mohsen, Engy

doi:10.1016/j.sjbs.2020.03.007

Cited by 29 publications

(22 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To reduce errors, only the clear and distinguishable bands were scored. The bands were scored as present (1) or absent (0) to create the binary dataset [ 76 ]. The polymorphism percentage was calculated by dividing the number of amplified polymorphic bands by the total number of amplified bands by the same primer or primer combination.…”

Section: Methodsmentioning

confidence: 99%

Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection

Atia

El‐Khateeb

El-Maksoud

et al. 2021

Plants

View full text Add to dashboard Cite

Wheat is a major nutritional cereal crop that has economic and strategic value worldwide. The sustainability of this extraordinary crop is facing critical challenges globally, particularly leaf rust disease, which causes endless problems for wheat farmers and countries and negatively affects humanity’s food security. Developing effective marker-assisted selection programs for leaf rust resistance in wheat mainly depends on the availability of deep mining of resistance genes within the germplasm collections. This is the first study that evaluated the leaf rust resistance of 50 Egyptian wheat varieties at the adult plant stage for two successive seasons and identified the absence/presence of 28 leaf rust resistance (Lr) genes within the studied wheat collection. The field evaluation results indicated that most of these varieties demonstrated high to moderate leaf rust resistance levels except Gemmeiza 1, Gemmeiza 9, Giza162, Giza 163, Giza 164, Giza 165, Sids 1, Sids 2, Sids 3, Sakha 62, Sakha 69, Sohag 3 and Bany Swif 4, which showed fast rusting behavior. On the other hand, out of these 28 Lr genes tested against the wheat collection, 21 Lr genes were successfully identified. Out of 15 Lr genes reported conferring the adult plant resistant or slow rusting behavior in wheat, only five genes (Lr13, Lr22a, Lr34, Lr37, and Lr67) were detected within the Egyptian collection. Remarkedly, the genes Lr13, Lr19, Lr20, Lr22a, Lr28, Lr29, Lr32, Lr34, Lr36, Lr47, and Lr60, were found to be the most predominant Lr genes across the 50 Egyptian wheat varieties. The molecular phylogeny results also inferred the same classification of field evaluation, through grouping genotypes characterized by high to moderate leaf rust resistance in one cluster while being highly susceptible in a separate cluster, with few exceptions.

show abstract

Section: Methodsmentioning

confidence: 99%

Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection

Atia

El‐Khateeb

El-Maksoud

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…Moreover, Abouseadaa et al . assessed the genetic relationships of 16 species belonging to two major genera of Mammillaria and Notocactus under the family Cactaceae 29 using two modern gene‐targeting marker techniques, i.e. SCoT polymorphism and CDDP.…”

Section: Discussionmentioning

confidence: 99%

Gene‐targeted molecular phylogeny, phytochemical analysis, antibacterial and antifungal activities of some medicinal plant species cultivated in Egypt

Omar

Elsayed

Reyad

et al. 2020

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction Medicinal plants have been used in healthcare since time immemorial, as have their therapeutic activities and the production of plant‐based medicines. Objectives This study aims to use gene‐targeted molecular markers for genetic diversity analysis of 16 medicinal plants. Besides, phytochemical analysis antibacterial and antifungal activities of some medicinal plant extracts commonly used in Egypt are compared to major compounds. Methods DNA‐based classification of 16 medicinal species using Conserved DNA‐Derived Polymorphism (CDDP) and Start Codon Targeted (SCoT) primers. Three species representing three orders (Pelargonium graveolens, Matricaria chamomilla, and Hyoscyamus muticus were analysed [high‐performance liquid chromatography (HPLC), gas chromatography‐mass spectrometry (GC‐MS)] and evaluated for their antibacterial and antifungal activities against (Escherichia coli O157: H7 ATCC 93111, Salmonella typhimurium ATCC 14028, Methicillin‐resistant Staphylococcus aureus (MRSA) ATCC 43300, Bacillus ceruse ATCC 33018, and Sclerotinia sclerotiorum in comparison with some of their antimicrobial components. Results Our results revealed 309 and 349 polymorphic bands with 100% polymorphism. Among them, 51 and 57 were unique loci for CDDP and SCoT, respectively. The 16 species were categorised into three groups depending on the similarity matrix. The results of antibacterial and antifungal activities revealed that Pelargonium oil showed significant antifungal and antibacterial activities against the tested pathogens. Gallic acid severely reduced all tested bacteria's growth, but atropine severely reduced the growth of the B. ceruse only. Molecular modelling revealed their activity against sclerotium development. Conclusion The gene‐targeted marker techniques were highly useful tools for the classification of the 16 medicinal plant species, despite displaying high similarities at morphological and phytochemical analyses but, have antifungal and antibacterial activities.

show abstract

“…To generate a binary data set, the amplicons were scored as absent (0) and present (1). The polymorphism percentage was analyzed by dividing the number of amplified polymorphic bands by the total number of amplified bands separately for each primer [42]. A similarity matrix was built to estimate the genetic distances between all possible treatment pairs.…”

Section: Molecular Data Analysismentioning

confidence: 99%

Nano-Insecticides Against the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae): Toxicity, Development, Enzyme activity, and DNA Mutagenicity

Awad

Ibrahim

Osman

et al. 2021

Preprint

View full text Add to dashboard Cite

High-frequency doses of chemical pesticides cause environmental pollution with high pesticide residues. In addition, increasing insecticide resistance in many insect pests requires novel pest control methods. Nanotechnology could be a promising field of modern agriculture, and is receiving considerable attention in the development of novel nano-agrochemicals, such as nanoinsectticides and nanofertilizers. This study assessed the effects of the lethal and sublethal concentrations of chlorantraniliprole, thiocyclam, and their nano-forms on the development, reproductive activity, oxidative stress enzyme activity, and DNA changes at the molecular level of the polyphagous species of black cutworm Agrotis ipsilon. The results revealed that A. ipsilon larvae were more susceptible to the nano-formsthan the regular forms of both nano chlorine and sulfur within the chlorantraniliprole and thiocyclam insecticides, respectively, with higher toxicities than the regular forms (ca. 3.86, and ca.2.06-fold, respectively). Significant differences in biological parameters, including developmental time and reproductive activity (fecundity and hatchability percent) were also observed. Correspondingly, increases in oxidative stress enzyme activities were observed, as were mutagenic effects on the genomic DNA of A. ipsilon after application of the LC50 of the nano-forms of both insecticides compared to the control. The positive results obtained here have led us to apply these nano-forms indifferent insect models in additional studies.

show abstract

Gene-targeted molecular phylogeny, phytochemical profiling, and antioxidant activity of nine species belonging to family Cactaceae

Cited by 29 publications

References 27 publications

Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection

Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection

Gene‐targeted molecular phylogeny, phytochemical analysis, antibacterial and antifungal activities of some medicinal plant species cultivated in Egypt

Nano-Insecticides Against the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae): Toxicity, Development, Enzyme activity, and DNA Mutagenicity

Contact Info

Product

Resources

About