Gene therapy and reproductive medicine

Towards Fibroid Gene Therapy: Adenovirus-Mediated Delivery of Herpes Simplex Virus 1 Thymidine Kinase Gene/Ganciclovir Shrinks Uterine Leiomyoma in the Eker Rat Model

“…Gynecology and gynecologic oncology are at the forefront of evolving gene therapy towards both benign and malignant disease [86,87] . Gynecologic gene therapy has advanced to human clinical trials for ovarian carcinoma [86,87] ; a phase III human clinical trial has begun in patients with stage III epithelial ovarian cancer [88] .…”

“…Gynecologic gene therapy has advanced to human clinical trials for ovarian carcinoma [86,87] ; a phase III human clinical trial has begun in patients with stage III epithelial ovarian cancer [88] . Similar strategies are at early stages, for endometrial carcinoma [89][90] , breast cancer [91] , cervical cancer [92] , and other benign gynecological disorders such as endometriosis [93] , ovarian failure [94] and adhesion [95] .…”

“…Gene therapy success for a genetic disease -severe combined immunodeficiency disease -has been achieved [86,96] , and ongoing gene therapy development includes choosing the appropriate gene, method of gene delivery, achieving temporary or stable transfection, regulating gene expression, avoiding side effects and achieving better results than conventional therapy [47,50,71,78,79,86] .…”

In Vivo Gene Transfer in the Female Bovine: Potential Applications for Biomedical Research in Reproductive Sciences

“…Most of the gene transfer research has been conducted on several non-reproductive topics including blood diseases (Nienhuis, 2008), neurological dysfunctions (Manfredsson & Mandel, 2010), cancer (Pei et al, 2010), lung diseases (Geiger et al, 2010), bone healing (Evans, 2010), skin diseases (Long et al, 2009) and heart failure (Poller et al, 2010). To a lesser extent gene transfer research in gynecological diseases (Raki et al, 2006;Hassan et al, 2009) and reproductive medicine (Stribley et al, 2002;Daftary & Taylor, 2003;Yoshimura et al, 2010) has been undertaken using the mouse as model species. Although the mouse model possesses several advantages (e.g.…”

“…The physiology, organ size, genome organization, life span and pathology of farm animal species reflect the human situation much better than rodent models (Casal & Haskins 2006;Habermann et al, 2007;Jacobsen et al, 2010;Muschler et al, 2010). Implementation of in vivo gene transfer technology in relevant large animal models is pivotal to elucidate molecular pathways involved in reproductive processes such as ovarian involves the in vitro transfer of exogenous genetic material into cells followed by the in vivo delivery of the genetically modified cell into the target tissue (Yang, 1992;Stribley et al, 2002;Gardlík et al, 2005). In vivo gene therapy makes reference to the direct transfer of nucleic acids into target cells (Yang, 1992;Stribley et al, 2002;Gardlík et al, 2005).…”