Gene Therapy for Cardiovascular Disease: Inserting New Genes, Regulating the Expression of Native Genes, and Correcting Genetic Defects

Jovin, Ion S.; Giordano, Frank J.

doi:10.1002/9780470691977.ch9

Cited by 1 publication

(1 citation statement)

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“…Although the studies did not directly focus on the biological consequences of DNA methylation, long‐term effects of in vivo gene therapy have been demonstrated. One such possibility is that adeno‐associated virus may take advantage of viral machinery, in part, via the effects of their inverted terminal repeats, which may have the ability to protect the therapeutic DNA from unwanted degradation in the host cell (55). In higher eukaryotes, gene expression is regulated autonomously through its entire system.…”

Section: Discussionmentioning

confidence: 99%

Nanoparticle‐mediated rhodopsin cDNA but not intron‐containing DNA delivery causes transgene silencing in a rhodopsin knockout model

et al. 2015

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Previously, we compared the efficacy of nanoparticle (NP)-mediated intron-containing rhodopsin (sgRho) vs. intronless cDNA in ameliorating retinal disease phenotypes in a rhodopsin knockout (RKO) mouse model of retinitis pigmentosa. We showed that NPmediated sgRho delivery achieved long-term expression and phenotypic improvement in RKO mice, but not NP housing cDNA. However, the protein level of the NPsgRho construct was only 5-10% of wild-type at 8 mo postinjection. To have a better understanding of the reduced levels of long-term expression of the vectors, in the present study, we evaluated the epigenetic changes of subretinal delivering NP-cDNA vs. NP-sgRho in the RKO mouse eyes. Following the administration, DNA methylation and histone status of specific regions (bacteria plasmid backbone, promoter, rhodopsin gene, and scaffold/ matrix attachment region) of the vectors were evaluated at various time points. We documented that epigenetic transgene silencing occurred in vector-mediated gene transfer, which were caused by the plasmid backbone and the cDNA of the transgene, but not the intron-containing transgene. No toxicity or inflammation was found in the treated eyes. Our results suggest that cDNA of the rhodopsin transgene and bacteria backbone interfered with the host defense mechanism of DNA methylation-mediated transgene silencing through heterochromatin-associated modifications.-Zheng, M., Mitra, R. N., Filonov, N. A., Han, Z. Nanoparticle-mediated rhodopsin cDNA but not intron-containing DNA delivery causes transgene silencing in a rhodopsin knockout model. FASEB J. 30, 1076-1086 (2016). www.fasebj.org

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Section: Discussionmentioning

confidence: 99%