Gene transfer in the marine environment

Hermansson, M

doi:10.1016/0168-6496(94)90024-8

Cited by 3 publications

(3 citation statements)

References 34 publications

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“…Nevertheless, there is little information about the survival of the bacterial population (enteric and wastewater bacteria) in the receiving waters after treated wastewater effluents discharge. Moreover, the increment of resistance plasmid-bearing strains detected in natural systems has led scientists to propose several hypotheses about their origin: selection in the wastewater treatment plants (Armstrong et al 1982;Murray et al 1984;Walter and Vennes 1985), transfer processes in plants or in receiving waters (Young 1993;Hermansson and Linberg 1994), or the ability to survive in recipient channels (Awong et al 1990;Barcina et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Discharge of disinfected wastewater in recipient aquatic systems: fate of allochthonous bacterial and autochthonous protozoa populations

et al. 1998

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The discharge of disinfected effluents affects the bacterivorous ability of protozoa and the effect depends on the disinfectant applied. Chlorine provokes a decrease in the number of protozoa and a delay in the bacterivorous ability. The discharge of ozonated and peracetic acid-treated wastewater provokes only an initial slight decrease in bacterivorous ability. No correlation was found between toxicity values detected using the Microtox assay and the effect of disinfected effluents on freshwater protozoa population. After the disinfection processes, recipient systems (fresh and marine water) have different effects on the survival of Escherichia coli populations discharged to them. The effect of the freshwater recipient system is less negative than the effect provoked by sea-water, and the differences detected depend on the disinfection treatment applied. The wastewater bacterial population as a whole is able to grow after discharge of disinfected wastewater to receiving waters. However, in the absence of predation or competition, the recipient systems exert selection, with rod-shaped bacteria predominating.

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Section: Introductionmentioning

confidence: 99%

Discharge of disinfected wastewater in recipient aquatic systems: fate of allochthonous bacterial and autochthonous protozoa populations

et al. 1998

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“…The concentration and bioavailability of D‐DNA has both evolutionary and ecological significance. Double stranded D‐DNA has the potential to alter the genetic make‐up of microbial cells by the process of natural transformation (Hermansson and Linberg 1994) or, if hydrolyzed, can serve as a source of nitrogen and phosphorus (Jørgensen and Jacobsen 1996), macronutrients that are often limiting to microorganisms in open ocean ecosystems (Karl and Church 2014). As a consequence, there has been considerable interest in developing methods to investigate the nature and dynamics of this component of the larger DOM pool.…”

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confidence: 99%

A method for characterizing dissolved DNA and its application to the North Pacific Subtropical Gyre

Linney

Schvarcz

Steward

et al. 2021

Limnology & Ocean Methods

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Dissolved DNA (D-DNA) is a ubiquitous component of dissolved organic matter in aquatic systems. It is operationally defined as the DNA that passes a membrane filter and thus includes pools of truly dissolved "free" DNA (F-DNA), virion encapsidated DNA, DNA within membrane vesicles, and possibly other bound forms, each with different sources and lability. We investigated whether filtration (< 0.1 μm), concentration by tangential flow ultrafiltration (> 30 kDa), and fractionation in an equilibrium buoyant density gradient could be used to discriminate the mass contributions of the different pools of filterable DNA in seawater. Spike-in experiments with a known range of DNA standards (75-20,000 bp) indicated that this method results in high recoveries of F-DNA (68-86%) with minimal degradation. Application of the fractionation method to seawater samples collected from the oligotrophic North Pacific Ocean followed by analysis of fractions (epifluorescence and electron microscopy, DNase digestion) suggested that the low-density fractions (1.30-1.35 g mL −1) were dominated by vesicle-like particles, mid-density fractions (1.45-1.55 g mL −1) by virus-like particles, and high-density fractions (1.60-1.70 g mL −1) by F-DNA. The estimated concentration of DNA that is either F-DNA, in viruses, or in vesicles was 0.13, 0.14, and 0.08 μg L −1 , respectively in the euphotic zone and 0.09, 0.04, and 0.03 μg L −1 , respectively in the mesopelagic zone. The approach described should be useful for more detailed investigations of the abundance, dynamics, and sources of DNA in the distinct pools that comprise filterable DNA in aquatic environments. Dissolved DNA (D-DNA)-a highly labile and nutrient-rich component of dissolved organic material (DOM)-is ubiquitous in all aquatic habitats investigated to date, including freshwater rivers and lakes (Pillai and Ganguly 1972), coastal marine and estuarine systems (DeFlaun et al. 1986), sediments (Dell'Anno et al. 2002), and the open ocean (Karl and Bailiff 1989; Brum 2005). Concentrations of D-DNA often represent a substantial fraction of the total DNA (DeFlaun et al. 1987; Karl and Bailiff 1989), especially in the open ocean. Spatial patterns of D-DNA concentrations in the ocean are similar to those of total microbial biomass and total DOM, decreasing with distance from shore and with depth (DeFlaun et al. 1987; Karl and Bailiff 1989; Weinbauer et al. 1993, 1995). The concentration and bioavailability of D-DNA has both evolutionary and ecological significance. Double stranded D-DNA has the potential to alter the genetic make-up of microbial cells by the process of natural transformation (Hermansson and Linberg 1994) or, if hydrolyzed, can serve as a source of nitrogen and phosphorus (Jørgensen and Jacobsen 1996), macronutrients that are often limiting to microorganisms in open ocean ecosystems (Karl and Church 2014). As a consequence, there has been considerable interest in developing methods to investigate the nature and dynamics of this component of the larger DOM pool. Methods to stud...

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“…It can be taken up as whole polydeoxyribonucleotides (>10 kb) (9-11), as well as, hydrolyzed and broken down to nucleotides and nucleic acid bases (12)(13)(14). Alternatively, the genomic information encoded by D-DNA has the potential to be integrated by natural transformation (15).…”

Section: Introductionmentioning

confidence: 99%

Microbial sources of exocellular DNA in the ocean

Linney¹,

Eppley

Romano

et al. 2021

Preprint

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Dissolved DNA (D-DNA) is operationally defined as DNA that passes through a 0.1 micrometer filter. It is composed of vesicles, viruses, and exocellular free DNA (F-DNA) and is ubiquitous in all aquatic systems, although the sources, sinks, and ecological consequences are largely unknown. Using a method that provides separation of vesicles, viruses, and F-DNA, we compared open ocean depth profiles of metagenomic DNA associated with each fraction. Pelagibacter-like DNA dominated the vesicle fractions for all samples examined over a depth range of 75–500 m. The viral DNA fraction was composed predominantly of myovirus-like and podovirus-like DNA, and contained the highest proportion of unannotated sequences. Euphotic zone F-DNA (75–125 m) contained mostly bacterial and viral sequences, with bacteria dominating in the mesopelagic (500–1000 m). A high proportion of mesopelagic (500 and 1000 m) F-DNA sequences appeared to originate from surface waters, including a large amount of DNA contributed by high-light Prochlorococcus species. Throughout the water-column, but especially in the mesopelagic, the composition of F-DNA sequences was not always reflective of co-occurring microbial communities that inhabit the same sampling depth. These results highlight the composition of F-DNA in different regions of the water-column (euphotic and mesopelagic zones), with implications for dissolved organic matter cycling and export (by way of sinking particles and/or migratory zooplankton) as a delivery mechanism.

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Gene transfer in the marine environment

Cited by 3 publications

References 34 publications

Discharge of disinfected wastewater in recipient aquatic systems: fate of allochthonous bacterial and autochthonous protozoa populations

Discharge of disinfected wastewater in recipient aquatic systems: fate of allochthonous bacterial and autochthonous protozoa populations

A method for characterizing dissolved DNA and its application to the North Pacific Subtropical Gyre

Microbial sources of exocellular DNA in the ocean

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