Gene Transfer of Calcitonin Gene–Related Peptide Suppresses Development of Allograft Vasculopathy

Zhang, X.N.; Chen, D.Z.; Zheng, Yuhui; Liang, Jiangang; Yang, H.X.; Lin, Xinhua; Zhuang, Jian

doi:10.1016/j.transproceed.2008.12.038

Cited by 4 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CGRP was significantly upregulated in the sheath. As a neuropeptide found in eye aqueous humor, an IP site, it has been observed to suppress nitric oxide production by macrophages and protect allogeneic tissue transplants (Taylor et al, 1998;Zhang et al, 2009). We found that HLAG mRNA was significantly upregulated in the human HF; a previous study showed protein expression in mouse HFs (Paus et al, 1994).…”

Section: Differential Expression Of Immunoregulatory Factors In Hfssupporting

confidence: 47%

Somatostatin Expression in Human Hair Follicles and Its Potential Role in Immune Privilege

Breitkopf

Leung

et al. 2013

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Immune privilege (IP) is believed to exist in the anagen hair follicle (HF). Studies have shown that downregulation of major histocompatibility complex Class I occurs and immunosuppressive factors are expressed in the HF bulb and bulge. However, demonstration and quantification of functional IP in HF cells are required. We examined the middle (sheath) and lower (bulb) portions of the human HF using quantitative real-time RT-PCR (qPCR), immunohistology, ELISA, in vitro coculture with peripheral blood mononuclear cells (PBMCs), and flow cytometry. We found that HF cells, relative to non-follicular epidermal cells, failed to promote allogeneic PBMC proliferation and CD4(+) and CD8(+) IFNγ production. By qPCR, we found significant downregulation of Class I and Class II HLA alleles in both the bulb and sheath, and upregulation of multiple immunoregulatory genes. It is noteworthy that somatostatin (SST) was significantly upregulated relative to epidermis. By immunohistochemistry, SST was most strongly expressed in the HF outer root sheath, and, by ELISA, cultured sheath cells secreted SST. PBMCs, cultured with stimulatory allogeneic epidermal cells and SST, secreted significantly less IFNγ than controls. Addition of SST antagonists to PBMCs cocultured with allogeneic HF cells increased IFNγ secretion. The data identify SST as a secretory factor potentially contributing to the HF IP repertoire.

show abstract

Section: Differential Expression Of Immunoregulatory Factors In Hfssupporting

confidence: 47%

Somatostatin Expression in Human Hair Follicles and Its Potential Role in Immune Privilege

Breitkopf

Leung

et al. 2013

Journal of Investigative Dermatology

View full text Add to dashboard Cite

show abstract

“…To investigate the possibility of an alternate anti-inflammatory route, we focused on NGF, as enhanced NGF production is associated with anti-inflammatory effects, including the suppression of neutrophils or macrophage infiltration and IL-10 production in intestinal epithelial cells ( 28 , 29 , 30 ). In addition, the functional blockade of NGF worsens experimental colitis by decreasing calcitonin gene-related peptide, which enhances iNOS release from proliferated lymphocytes ( 28 , 31 , 32 ). The NGF-induced phosphorylation of protein kinase B inhibits the translocation of NF-κB, an iNOS inducer, into the nucleus ( 33 , 34 ).…”

Section: Discussionmentioning

confidence: 99%

Hangekobokuto, a traditional Japanese herbal medicine, ameliorates postoperative ileus through its anti-inflammatory action

Endo

Oikawa

Tonooka

et al. 2022

J Smooth Muscle Res

View full text Add to dashboard Cite

Background/Aims: Gastroprokinetic agents are used for patients with postoperative ileus (POI), and the Japanese traditional herbal medicine daikenchuto (DKT) is one such agent used in the clinical setting.POI is caused by inflammation. DKT and rikkunshito have anti-inflammatory abilities in addition to their gastroprokinetic effects. The efficacy of Kampo formulations, including hangekobokuto (HKT), in patients with POI has been reported recently. Several authors have described the efficacy of honokiol, the primary component of Magnoliae Cortex, in HKT in mouse models of POI. We therefore analyzed the effect of HKT on POI model mice to determine the similarities in the mechanism of action between HKT and DKT.Methods: HKT was administered orally to each mouse before and after intestinal manipulation was performed on the distal ileum. The gastrointestinal transit in vivo, leukocyte infiltration, and levels of inflammatory mediators, such as cytokines and chemokines, were analyzed.Results: HKT significantly inhibited the infiltration of neutrophils and macrophages and led to the recovery of delayed intestinal transit. In addition, it significantly decreased inducible nitric oxide synthase (iNOS) as well as honokiol levels, suggesting anti-inflammatory activity. However, it did not inhibit the increase in levels of interleukin (IL)-1beta and IL-6, which are related to iNOS induction. In contrast, HKT increased levels of nerve growth factor (NGF) and suppressed those of nuclear factor-κB (NFκB), which are related to

show abstract

Early adventitial activation characterized by NADPH oxidase expression and neovascularization in an aortic transplantation model

Sun

Guo

et al. 2016

Experimental and Molecular Pathology

View full text Add to dashboard Cite

Gene Transfer of Calcitonin Gene–Related Peptide Suppresses Development of Allograft Vasculopathy

Cited by 4 publications

References 22 publications

Somatostatin Expression in Human Hair Follicles and Its Potential Role in Immune Privilege

Somatostatin Expression in Human Hair Follicles and Its Potential Role in Immune Privilege

Hangekobokuto, a traditional Japanese herbal medicine, ameliorates postoperative ileus through its anti-inflammatory action

Early adventitial activation characterized by NADPH oxidase expression and neovascularization in an aortic transplantation model

Contact Info

Product

Resources

About