Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea

Kumari, Aloka; Baskaran, P.; Staden, J. Van

doi:10.1590/1984-70332017v17n3a35

Cited by 3 publications

(2 citation statements)

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“…Several genetic transformation techniques have been developed to genetically engineer plants that can tolerate environmental stress, and improve productivity and quality of plant species, although the efficiency of current gene transfer techniques is still low (Kumari et al 2017). Before approval for cultivation and commercialization, usually a new GM event must undergo a risk assessment, where not only the recombinant construct's integration, stability, and expression must be demonstrated, but also evaluated for possible unintended genetic and phenotypic abnormalities compared to the most similar non-GM counterpart available (Bartsch et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Comparison of in vitro callus-cultures from transgenic maize AG-5011YG (MON810) and conventional near-isogenic maize AG-5011

Holderbaum

Traavik

Nodari

et al. 2019

Crop Breed. Appl. Biotechnol.

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MON810 is a genetically-modified (GM) maize (Zea mays) event commonly employed in insect-resistant GM maize hybrids. GM events obtained by biolistic transformation methods, such as MON810, are generated by insertion of a recombinant gene expression cassette in a random locus of the plant genome, and this process may cause emergent properties besides the intended modification. Here, we compared morphophysiological parameters of MON810 GM maize hybrid AG-5011YG and its non-GM near-isogenic hybrid (NIH) AG-5011, using in vitro cultures as an interactive model. NIH callogenesis frequency, callus friability, and de novo morphogenesis were compared using two explant types and different 2,4-dichlorophenoxyacetic acid (2,4-D) levels. 2,4-D modulated the morphophysiological responses of both NIHs, but when using root segments as explants, the GM showed significantly different trends for callus induction and friability, with lower responses at higher 2,4-D concentrations, indicating an emergent property related to altered cell response to 2,4-D.

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Section: Introductionmentioning

confidence: 99%

Comparison of in vitro callus-cultures from transgenic maize AG-5011YG (MON810) and conventional near-isogenic maize AG-5011

Holderbaum

Traavik

Nodari

et al. 2019

Crop Breed. Appl. Biotechnol.

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“…However, the content of Vigna radiata plant defensin 1 (VrPDF1) in mung bean seeds is very low, hence an increase in VrPDF1 content in mung bean seeds leads to enhance alpha -amylase inhibition and improve weevil resistance of mung bean. Transformation is considered a technological measure which enhances protein expression in transgenic plants (Dao Xuan Tan et al, 2015;Thanh Son Lo et al, 2015) and transgenic technique can also be applied to biotechnological crop improvement of the same species and potentially to other plants (Aloka Kumari et al, 2017). In the direction of approach overexpression of defensin protein in seeds, Swathi et al (2008) successfully expression of defensin gene in tobacco and peanut to enhance antifungal activity in transgenic plants.…”

Section: Introductionmentioning

confidence: 99%

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Supplemental Information 1: Analysis of a-Amylase Activity of Mung Bean Weevils (IU Mg-) and Analysis of Content of Recombinant

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Plant defensins are known for different biological functions such as insect resistance, antibacterial, antifungal and antiviral activities. The role of plant defensins against weevils is based on inhibitor of alpha-amylase activity in weevil gut, therefore, weevils can not digest starch and then die. The low content of Vigna radiata plant defensin 1 (VrPDF1) in mung bean seeds is promted researches to increase the VrPDF1content, in which leads to enhance alpha-amylase inhibition and improve bruchid resistance in mung bean. This article presents the results of overexpression of VrPDF1 gene in transgenic mung bean plants. VrPDF1 gene was successfully transferred into DX22 mung bean cultivar and expressed in T1 generation transgenic mung bean seeds. The extract containing recombinant VrPDF1 protein inhibited alpha-amylase of weevil larvae in its larval stage. The performance of alpha-amylase inhibitor of DX1-3 and DX1-7 transgenic mung bean lines increased by 166.40% and 178.19% respectively, in comparison with non-transgenic plants. The enhancement of alpha-amylase inhibitor ability of rVrPDF1 extracted from transgenic plants is scientifically fundamental to confirm the effectiveness of the application of gene technology in enhancement the ability of mung bean weevil resistance in particular and grain crops in general revenue.

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