2010
DOI: 10.1016/s0076-6879(10)77013-0
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Gene Trap Mutagenesis in the Mouse

Abstract: Gene trapping in mouse embryonic stem (ES) cells is an efficient method for the mutagenesis of the mammalian genome. Insertion of a gene trap vector disrupts gene function, reports gene expression, and provides a convenient tag for the identification of the insertion site. The trap vector can be delivered to ES cells by electroporation of a plasmid, by retroviral infection, or by transposon-mediated insertion. Recent developments in trapping technology involve the utilization of site-specific recombination sit… Show more

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Cited by 40 publications
(32 citation statements)
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“…Lpin2-deficient mice were generated by targeted trapping (39) in ES cells. ES cells were electroporated with the trapping construct consisting of a promoterless β-geo gene (fusion of a β-gal reporter and a neomycin- Fig.…”
Section: Methodsmentioning
confidence: 99%
“…Lpin2-deficient mice were generated by targeted trapping (39) in ES cells. ES cells were electroporated with the trapping construct consisting of a promoterless β-geo gene (fusion of a β-gal reporter and a neomycin- Fig.…”
Section: Methodsmentioning
confidence: 99%
“…Each of these methods has advantages and disadvantages; for example, although chemical mutagenesis generates unbiased mutations, these mutations are typically single base-pair changes and can be difficult (or sometimes impossible) to specifically identify. In contrast, TE-induced mutations are easier to map (for review, see Friedel and Soriano 2010;Ammar et al 2012;St Johnston 2013). However, TEs often preferentially insert at particular sites, leading to TE ''hot spots'' and the lack of mutations in other genomic regions.…”
Section: Bringing Surgical Precision To Genetic Screensmentioning
confidence: 99%
“…Gene trap vectors also facilitate the selection of cells with insertions into genes. This is achieved by introducing a selectable marker downstream of the splice acceptor site in the message of the trapped gene (Friedel & Soriano 2010). Expression of the marker depends on the level of expression of the trapped gene and the position of the vector within the locus.…”
Section: Application Of Haploid Cell Systems For Genetic Screensmentioning
confidence: 99%