General and Molecular Cytology

Franke, Werner W.; Scheer, Ulrich; Herth, W.

doi:10.1007/978-3-662-12810-7_1

Cited by 4 publications

(2 citation statements)

References 193 publications

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“…The composition of fractions from the two onion tissues is given in Table 1. The DNA, RNA, protein and phospholipid contents of nuclear fractions are in basic agreement with data reported for nuclei from other plant materials (for reviews see 25,30,85,94,97). The composition of microsomal fractions is close to that reported for rER-derived fractions from a variety of plant materials (4, 61; for further references see 32), but this fraction is distinguished by its RNA content from "smooth vesicle" fractions from plants (32,61).…”

Section: Gross Chemical Composition and Buoyant Densitysupporting

confidence: 86%

Characterization of nuclear membranes and endoplasmic reticulum isolated from plant tissue.

Philipp¹,

Franke²,

Keenan³

et al. 1976

The Journal of Cell Biology

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Nuclei, nuclear membranes and rough endoplasmic reticulum (rER) were isolated from onion root tips and stems. Structural preservation and purity of the fractions was determined by electron microscopic and biochemical methods. Gross compositional data (protein, phospholipid, nonpolar lipids, sterols, RNA, DNA), phospholipid and fatty acid patterns, enzyme activities (ATPases, ADPase, IDPase, glucose-6-phosphatase, 5'-nucleotidase, acid phosphatase, and NADH-and NADPH-cytochrome c reductases), and cytochrome contents were determined. A stable, high salt-resistant attachment of some DNA with the nuclear membrane was observed as well as the association of some RNA with high salt-treated nuclear and rER membranes. The phospholipid pattern was identical for both nuclear and rER membranes and showed a predominance of lecithin (about 60%) and phosphatidyl ethanolamine (20-24%). Special care was necessary to minimize lipid degradation by phospholipases during isolations, Nonpolar lipids, mostly sterols and triglycerides, accounted for 35-45% of the membrane lipids. Sterol contents were relatively high in both membrane fractions (molar ratios of sterols to phospholipids ranged from 0.12 to 0.43). Sitosterol accounted for about 80% of the total sterols. Palmitic, oleic, and linoleic acids were the most prevalent acids in membrane-bound lipids as well as in storage lipids and occurred in similar proportions in phospholipids, triglycerides and free fatty acids of the membrane. About 80% of the fatty acids in membrane phospholipids and triglycerides were unsaturated. A cytochrome of the b5 type was characterized in these membranes, but P-450-1ike cytochromes could not be detected. Both NADH and NADPHcytochrome c reductases were found in nuclear and rER membranes and appeared to be enriched in rER membranes. Among the phosphatases, Mg2+-ATPase and, to lesser extents, ADPase, IDPase and acid phosphatase activities occurred in the fractions, but significant amounts of monovalent ion-stimulated ATPase, 5'-nucleotidase and glucose-6-phosphatase activities did not. The results obtained emphasize that the close biochemical similarities noted between rER and nuclear membranes of animal cells extend to these fractions from plant cells.

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Section: Gross Chemical Composition and Buoyant Densitysupporting

confidence: 86%

Characterization of nuclear membranes and endoplasmic reticulum isolated from plant tissue.

Philipp¹,

Franke²,

Keenan³

et al. 1976

The Journal of Cell Biology

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“…(1966) and the matrix material obtained by Kuratana (1974) Lamport, 1969Lamport, , 1970Lamport, , 1973Miller et , 1974). (Albersheim, 1975(Albersheim, , 1976Bateman and Basham, 1976;Cook and Stoddart, 1973;Franke et al. 1974;Lanç>ort, 1970;Northcote, 1972;Preston, 1974;Rayle and Zenk, 1973;Roland, 1973) and on cell wall biosynthesis (Chrispeels, 1976, Cocking, 1972Cook and Stoddart, 1973;Franke £t al., 1974;Karr, 1976;Loewus, 1973;Preston, 1974;O'Brien, 1972;Willison, 1976).…”

Section: Coccolith and Scale Chemistrymentioning

confidence: 99%

The in vivo and in vitro assembly of cell walls in the marine coccolithophorid, Hymenomonas carterae

Flesch

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Hvmenomonas carterae is a member of an ancient group of organisms, the coccolithophorids, so named because of their production of calcareous scales called coccoliths. The coccolithophorids have external appendages, flagella, and a haptonema; the haptonema is a bulbous structure found between the two flagella and may serve as an attachment organelle and aid in controlling movement (Manton, 1968). The coccolithophorids' habitat is predominantly marine with samples taken from tropic, temperate, and arctic seas (Pautard, 1970) where they help to make up a substantial portion of the plankton; one fresh water form has been reported (Manton and Peterfi, 1969). They also have made significant contributions to the fossil record (Pautard, 1970). Hvmenomonas has been referred to in the literature by the genus names Crlcosphaera and Svracosphaera (Paasche, 1968); for my work, I will use Hvmenomonas for the genus name following the recommendations of Manton and Peterfi (1969). These phytoflagellates are classified by the International Committee for Botanical Nomenclature as belonging in the order Coccolithophorales, in the class Haptophyceae, and in the phylum Chrysophyta and by the International Committee for Zoological Nomenclature as belonging in the order Coccolithophorida, in the class Mastigophora, and in the phylum Protozoa (Pautard, 1970). It is not the intention of this report to enter into this controversy. Reviews of coccolithophorids and Hvmenomonas can be found in Paasche (1968), Pautard (1970), and Williams (1972). 4 The life cycle of Hvmenomonas and the other coccolithophorids has been investigated by several authors (

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An examination of the relationship between freeze-fractured plasmalemma and cell-wall microfibrils

Willison

1976

Protoplasma

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General and Molecular Cytology

Cited by 4 publications

References 193 publications

Characterization of nuclear membranes and endoplasmic reticulum isolated from plant tissue.

Characterization of nuclear membranes and endoplasmic reticulum isolated from plant tissue.

The in vivo and in vitro assembly of cell walls in the marine coccolithophorid, Hymenomonas carterae

An examination of the relationship between freeze-fractured plasmalemma and cell-wall microfibrils

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