General mechanism of the chromatography of macromolecules: Experimental test of the universal nature of adsorption effects

Горбунов, А.А.; Solovyova, L. Ya.; Skvortsov, A.M.

doi:10.1016/s0032-3861(97)00282-6

Cited by 46 publications

(25 citation statements)

References 12 publications

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“…Therefore, from Equation (14) and data in Table 4-6 and 8, it is possible to calculate the gel volume fraction, b 3 , at the three selected hydrodynamic volumes in both types of column packings. The obtained values are presented as their inverse, that is, as the swelling degree of the gels (b À1…”

Section: (Iii) Influence Of the Polymer Solutementioning

confidence: 99%

Quantitative Evaluation of the Swelling and Crosslinking Degrees in Two Organic Gel Packings for SEC

Garcı́a

Recalde

Figueruelo

et al. 2001

Macromol. Chem. Phys.

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The elution behavior of different solvent/polymer systems has been studied by means of size‐exclusion chromatography (SEC) in two organic column packings based on polystyrene/divinylbenzene (PS/DVB) copolymer, named μ‐styragel and TSK‐Gel HHR. Both packings offer similar characteristics (pore size, particle size, efficiency) but some differences arise when eluting the same systems. The different elution behavior observed in both polymeric gels has been analyzed in terms of their swelling and crosslinking degrees. To evaluate the magnitude of these parameters, a new equation is proposed which has been derived from the Flory‐Huggins theory of swelling equilibrium, and takes into account binary and ternary thermodynamic interaction functions. The equation appropriately predicts the experimental results, showing that the gel that exhibits more solute‐packing attractive interactions (or adsorption as secondary mechanism) is also the one with the highest crosslinking degree or lowest swelling ratio. In this sense, the μ‐styragel column was the most crosslinked polymer network. In addition, the influence of solvent nature, polymer nature, and solute composition on the observed swelling degree has also been compared in both packings.

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Section: (Iii) Influence Of the Polymer Solutementioning

confidence: 99%

Quantitative Evaluation of the Swelling and Crosslinking Degrees in Two Organic Gel Packings for SEC

Garcı́a

Recalde

Figueruelo

et al. 2001

Macromol. Chem. Phys.

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“…According to this theory, the distribution coefficient K is a function of three parameters: the pore width D, the average radius of gyration R of the polymer coil and the adsorption interaction parameter c, which describes the interaction of a structural unit with stationary phase [35]. The interaction parameter is negative in SEC, where retention decreases with increasing molar mass and is positive in LAC, where retention increases exponentially with the number of repeat units.…”

Section: Separation Mechanisms In Lc Of Polymersmentioning

confidence: 99%

Full separation of oligomers in block copolymers of ethylene oxide and propylene oxide

Malik

Trathnigg

2009

J of Separation Science

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Original PaperFull separation of oligomers in block copolymers of ethylene oxide and propylene oxideIn this study di-and triblock copolymers of ethylene oxide (EO) and propylene oxide (PO) are analyzed by using CAP for one block and adsorption for the other block. This gives a complete picture of EO-and PO-based block copolymer with respect to the oligomers of both blocks. A full resolution of the individual oligomers can be achieved for both blocks up to an average molecular weight of 1000 -1500 of each block.

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“…It is measured in inverse length (nm -1 ). The value of c does not depend on D, V p , V i or on the degree of polymerization (DP) n, but strongly depends on mobile phase composition (for a given polymer -sorbent system), and on temperature [15].…”

Section: Introductionmentioning

confidence: 99%

A data base for polymer chromatography: Temperature dependence of interaction parameters in liquid adsorption chromatography

Trathnigg

Cuong

Ahmed

2009

J of Separation Science

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The temperature dependence of retention behaviour of polyethylene glycol (PEG) and its mono- and dimethyl ethers was studied on various RP columns in different mobile phases. The accessible volumes and the interaction parameters were determined from slope and intercept in a plot of the elution volumes of the oligomers of a polymer homologous series as a function of the difference of the elution volumes of consecutive oligomers. A quite different dependence of the interaction parameters was observed in the different mobile phases. While in methanol-water the interaction parameter decreases with increasing temperature, the opposite effect is observed in acetonitrile (ACN)-water. In acetone-water, the temperature dependence is almost negligible.

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General mechanism of the chromatography of macromolecules: Experimental test of the universal nature of adsorption effects

Cited by 46 publications

References 12 publications

Quantitative Evaluation of the Swelling and Crosslinking Degrees in Two Organic Gel Packings for SEC

Quantitative Evaluation of the Swelling and Crosslinking Degrees in Two Organic Gel Packings for SEC

Full separation of oligomers in block copolymers of ethylene oxide and propylene oxide

A data base for polymer chromatography: Temperature dependence of interaction parameters in liquid adsorption chromatography

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