General Method for the Detection of Cells producing Antibodies against Haptens and Proteins

Strausbauch, Paul H.; Sulica, Andrei; Givol, David

doi:10.1038/227068a0

Cited by 98 publications

(29 citation statements)

References 16 publications

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“…We wish, here, only to compare the efficacy of our plaquing procedure with that using other techniques of coupling haptens to SRBC. results (table VI) compare more than favorably with those given by Strausbauch et al [18]. Discussion The PFC method described here is a logical extension of the antiFyG PFC technique previously reported [12].…”

Section: Numbers Of Pfc Obtained In Mice Given One or More Injectionssupporting

confidence: 72%

A Simple Hemolytic Plaque Technique for the Enumeration of Anti-Hapten Antibody Forming Cells

Silver¹,

Miller²,

Warner³

1971

Int Arch Allergy Immunol

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Section: Numbers Of Pfc Obtained In Mice Given One or More Injectionssupporting

confidence: 72%

A Simple Hemolytic Plaque Technique for the Enumeration of Anti-Hapten Antibody Forming Cells

Silver¹,

Miller²,

Warner³

1971

Int Arch Allergy Immunol

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“…Antisera having an agglutination titer of 1:32-1:64 were pooled and the IgG fraction was isolated by DEAE-cellulose chromatography in 0.015 M phosphate buffer at pH 8.0. Fab' fragments of the anti-E, IgG were prepared as described by Strausbauch et al (15). F(ab')2 fragments of a polyvalent rabbit antihuman Fab fragment antisenim were identical to those used in a previous study (13).…”

Section: Methodsmentioning

confidence: 99%

A subpopulation of normal human peripheral B lymphcytes that bind IgE.

González-Molina¹,

Spiegelberg²

1977

J. Clin. Invest.

143

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“…AFC to SRC and DRC were detected by the Cunningham and Szenberg (27) modification of the Jerne plaque technique (28). The number of AFC to DNP was enumerated by separate assay of 0.1 ml samples of the cultured cell suspension with SRC and with SRC coated with rabbit anti-SRC Fab' conjugated with DNP (25,29). Optimal sensitivity was achieved by prior titration of the coupling ratios of Fab'-DNP to red cells.…”

Section: Methodsmentioning

confidence: 99%

The Relationship Between Antigenic Structure and the Requirement for Thymus-Derived Cells in the Immune Response

Feldmann

Basten

1971

The Journal of Experimental Medicine

259

140

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The relationship between the thymus and immunological competence of some rodents has been well documented (1), Both neonatal thymectomy and adult thymectomy followed by irradiation and bone marrow protection result in a diminished capacity to mount cell-mediated immune reactions. In addition some humoral antibody responses, for example to antigens such as heterologous erythrocytes (2), serum proteins (3-5), and haptenic determinants are impaired. In contrast other antigens, for example MS2 phage (6), Escherischia coli endotoxin (7), pneumococcal polysaccharide (8, 9), and polymerized flagellin (10) can stimulate relatively normal antibody production in thymectomized hosts, though frequently the range of antigen doses studied was limited. This paradox raises the problem of the role of thymusderived (T) 1 lymphocytes in those responses dependent on an intact thymus. Miller and Mitchell (11) have shown that T cells themselves do not secrete antibody in the classical sense but collaborate with thymus-independent (B) lymphocytes which then become antibodyforming cells (AFC). The importance of the helper function of T cells in antibody production in carrier-hapten systems has also been stressed by Mitchison (12), Benacerraf, and his colleagues (13,14) and Cheers et al. 2 However, this work does not clarify the difference between thymus-dependent and thymus-independent antibody responses.T h e purpose of the present communication is to examine this question in an in vitro mouse model using antigens of various physical forms. T h e flagellar

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General Method for the Detection of Cells producing Antibodies against Haptens and Proteins

Cited by 98 publications

References 16 publications

A Simple Hemolytic Plaque Technique for the Enumeration of Anti-Hapten Antibody Forming Cells

A Simple Hemolytic Plaque Technique for the Enumeration of Anti-Hapten Antibody Forming Cells

A subpopulation of normal human peripheral B lymphcytes that bind IgE.

The Relationship Between Antigenic Structure and the Requirement for Thymus-Derived Cells in the Immune Response

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