General Microbiology

Stanier, Roger Y.; Ingraham, John L.; Wheelis, Mark L.; Painter, Page R.

doi:10.1007/978-1-349-15028-1

Cited by 34 publications

(49 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It can, however, be used directly as a precursor in the synthesis of nucleotides. It was also accepted that polymerization of amino acids requires 4 rnol ATP (mol amino acid)-' (Stanier et al, 1987) rather than 2 mol.…”

Section: Calculation Of Yatpmentioning

confidence: 99%

Energetics of Saccharomyces Cerevisiae in Anaerobic Glucose-Limited Chemostat Cultures

Verduyn

Postma

Scheffers

et al. 1990

Journal of General Microbiology

228

170

View full text Add to dashboard Cite

The energetics of Saccharomyces cerenisiae were studied in anaerobic glucose-limited chemostat cultures via an analysis of biomass and metabolite production. The observed YATp was dependent on the composition of the biomass, the production of acetate, the extracellular pH, and the provision of an adequate amount of fatty acid in the medium. Under optimal growth conditions, the YATp was approximately 16 g biomass (mol ATP formed)-'. This is much higher than previously reported for batch cultures. Addition of acetic acid or propionic acid lowered the YATP. A linear correlation was found between the energy required to compensate for import of protons and the amount of acid added. This energy requirement may be regarded as a maintenance energy, since it was independent of the dilution rate at a given acid concentration.

show abstract

Section: Calculation Of Yatpmentioning

confidence: 99%

Energetics of Saccharomyces Cerevisiae in Anaerobic Glucose-Limited Chemostat Cultures

Verduyn

Postma

Scheffers

et al. 1990

Journal of General Microbiology

228

170

View full text Add to dashboard Cite

show abstract

“…Embora alguns contaminantes como o Lactobacillus plantarum possam agir de forma direta sobre o crescimento e desenvolvimento dos explantes, a maioria compromete o desenvolvimento normal dos cultivos de forma indireta, pois compete por nutrientes e vitaminas do meio de cultura e produz metabólitos fitotóxicos, tais como os ácidos láctico e acético, cianeto, além de certos reguladores de crescimento e antibióticos, que intoxicam os tecidos vegetais podendo levá-los à morte (Bakker & Schippers, 1987;Stanier et al, 1987;Kloepper et al, 1989;Leifert et al, 1989Leifert et al, , 1991Yepes & Aldwinckle, 1994).…”

Section: Introductionunclassified

Toxicidade de antibióticos no cultivo in vitro da batata em meios semi-sólido e líquido

Pereira

Fortes

2003

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi avaliar os efeitos fitotóxicos de antibióticos no crescimento e na taxa de multiplicação in vitro da batata. Brotações da cultivar Baronesa foram cultivadas em meio de multiplicação de consistência semi-sólida e líquida. O meio de multiplicação foi formado pelos sais e vitaminas de MS ao qual adicionou-se um dos seguintes antibióticos: ampicilina, cloranfenicol, estreptomicina e tetraciclina, previamente selecionados em razão da ação bactericida sobre contaminantes da cultura, nas concentrações de 0, 32, 64, 128, 256, 512 e 1.024 mg L -1 . Por 21 dias os materiais foram mantidos em sala de crescimento a 25±2°C, 16 horas de luz e fluxo de radiação de 35 µmol m -2 s -1 . Nos tratamentos em que se utilizou meio de cultura líquido, os frascos foram mantidos sob constante agitação em mesa agitadora do tipo orbital. A ampicilina foi o único antibiótico que não afetou a sobrevivência e o desenvolvimento dos explantes de batata em meio de multiplicação, podendo ser indicada para trabalhos de descontaminação in vitro dessa espécie. O aumento das concentrações de cloranfenicol, estreptomicina e tetraciclina no meio de cultura apresentou efeitos fitotóxicos severos sobre o crescimento e taxa de multiplicação do material vegetal.Termos para indexação: Solanum tuberosum, fitotoxicidade, micropropagação, antimicrobiano, contaminação. Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid mediaAbstract -The objective of this work was to evaluate the effects of antibiotics on the in vitro growth and multiplication rate of potato. Potato explants, cv. Baronesa, were cultivated in semi-solid and liquid multiplication culture medium. The medium was formed by MS salts, vitamins and one of the following antibiotics: ampicillin, chloramphenicol, streptomycin or tetracycline, previously selected by the bactericidal action on some culture contaminants, at 0, 32, 64, 128, 256, 512 and 1,024 mg L -1 . Each treatment had three replicates of ten explants. The materials were incubated for 21 days in a growth room at 25±2°C temperature, 16-hour-light and 35 µmol m -2 s -1 radiation. The flasks with liquid culture medium were kept under continuous agitation in an orbital shaker. Only ampicillin has not affected the survival and the normal development of potato explants in the culture medium, thereby being indicated for decontamination works in vitro of this specie. The increase of the concentrations of chloramphenicol, tetracycline and streptomycin presented severe phytotoxic effects on the growth and multiplication rate of potato explants.

show abstract

“…The maintenance of osmotic balance is critical for efficient physiological functioning of microbes (Stanier et al, 1987). It seems that the microbial cells studied in these experiments were able to maintain an osmotic balance with their micro environment thereby not allowing high salt concentrations to inhibit their essential metabolic processes.…”

Section: Cod Depletion Rate Impact Resultsmentioning

confidence: 92%

“…Moreover, the approximate range of sodium chloride concentration tolerated for growth for non halophiles lies in the range -0.0 -4.0 % g/ 100 ml or 0-40, 000 mg/L (Stanier et al, 1987).…”

Section: Cod Depletion Rate Impact Resultsmentioning

confidence: 99%