General morphology and capsid fine structure of African swine fever virus particles

Carrascosa, J.L.; Carazo, JoséM.; Carrascosa, Ángel L.; García, Narciso; Santisteban, Antonio; Viñuela, Eladio

doi:10.1016/0042-6822(84)90100-4

Cited by 146 publications

(114 citation statements)

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“…ASF virions have a complex structure consisting of a nucleoprotein core surrounded by a lipid membrane and the capsid which is enveloped by an external lipid membrane (Carrascosa et al, 1984). Virus proteins inserted into either the external virion membrane or the plasma membrane of infected cells may play an im-0001-2935 © 1995 SGM portant role through interacting with and stimulating the host's immune response.…”

Section: Introductionmentioning

confidence: 99%

African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats

Sun

Jacobs

Smith

et al. 1995

Journal of General Virology

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The African swine fever virus (ASFV)j 13L gene encodes a 177 amino acid protein (19.0 kDa) with a putative transmembrane domain between residues 32 and 52. There is a potential signal peptide cleavage site at residue 54 and several possible motifs for phosphorylation and myristylation. Rabbit antisera raised against a synthetic peptide from the C terminus of the j 13L ORF identified proteins of 25-27 kDa in cells infected with a recombinant vaccinia virus expressing the j 13L ORF, in ASFV-infected cells and in purified extracellular ASF virions. In ASFV-infected cells the jl3L protein was expressed late during infection and exhibited size variation (25-27 kDa) between the different ASFV strains. Nucleotide sequence analysis of the gene in these strains showed that these size differences were due to variation in the number and sequence of tandemly repeated amino acid repeats. Although ASFV-infected animals made antibodies to the jl3L protein, no protection was observed when pigs were vaccinated with a recombinant vaccinia virus expressing the j 13L ORF.

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Section: Introductionmentioning

confidence: 99%

African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats

Sun

Jacobs

Smith

et al. 1995

Journal of General Virology

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“…inner envelope) und das Kapsid. Das Kapsid besteht aus etwa 2.000 Kapsomeren (Carrascosa et al, 1984). Die Hauptkomponente der Kapsomere bildet das Protein 72 (p 72) (García-Escudero R, 1998), welches für den Eintritt des Virus in die Wirtszelle benötigt wird (Chapman et al, 2008).…”

Section: Das Virus Besitzt Mehrere Hüllen (Abbildung 1) Die Innersteunclassified

3.3 Afrikanische Schweinepest

Mayr¹

2007

Medizinische Mikrobiologie, Infektions- Und Seuchenlehre

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“…However, using p72 and other molecular markers such as 9RL ORF, Lubisi (2005) and Boshoff In infected cells, 81 acid and 14 basic proteins have been identified with a molecular weigh ranging between 10 and 220 kDa (Carrascosa et al, 1984, Carrascosa et al, 1985, Esteves et al, 1986). Like the mRNAs described previously the proteins can be also classified in three distinct groups: the early proteins synthesised before the DNA replication, the proteins synthesised all along the replication cycle and the late proteins.…”

Section: Genetic Characterization Of Asfv Isolatesmentioning

confidence: 99%

“…At late stages of the infection, the virus can infect other types of cells like megakaryocytes, platelets, neutrophils or hepatocytes (Carrasco et al, 1992, Fernandez et al, 1992a, Fernandez et al, 1992b. Moreover, some ASFV isolates have been In infected cells, 81 acid and 14 basic proteins have been identified with a molecular weigh ranging between 10 and 220 kDa (Carrascosa et al, 1984, Carrascosa et al, 1985, Esteves et al, 1986). Like the mRNAs described previously the proteins can be also classified in three distinct groups: the early proteins synthesised before the DNA replication, the proteins synthesised all along the replication cycle and the late proteins.…”

Section: Page 16mentioning

confidence: 99%

“…Virus particles are organized as a complex multi-layers structure (Carrascosa et al, 1984). Virions are composed by an 80 nm core shell containing the virus genome in a 30 nm nucleoid (forming the nucleoprotein system), surrounded by a first lipid layer (inner envelop) then by a protein layer C CF FP P/ /E EF FS SA A/ /A AH HA AW W/ /2 20 00 07 7/ /0 02 2 S Sc ci ie en nt ti if fi ic c r re ev vi ie ew w o on n A Af fr ri ic ca an n S Sw wi in ne e F Fe ev ve er r…”

Section: Virus Structurementioning

confidence: 99%

See 1 more Smart Citation

Scientific review on African Swine Fever

Sánchez‐Vizcaíno

Martínez‐López

Martínez‐Avilés

et al. 2009

EFS3

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The present document has been produced and adopted by the bodies identified above as authors. In accordance with Article 36 of Regulation (EC) No 178/2002, this task has been carried out exclusively by the authors in the context of a grant agreement between the European Food Safety Authority and the authors. The present document is published complying with the transparency principle to which the European Food Safety Authority is subject. It may not be considered as an output adopted by EFSA. EFSA reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors. The present document has been produced and adopted by the bodies identified above as authors. In accordance with Article 36 of Regulation (EC) No 178/2002, this task has been carried out exclusively by the authors in the context of a grant agreement between the European Food Safety Authority and the authors. The present document is published complying with the transparency principle to which the European Food Safety Authority is subject. It may not be considered as an output adopted by EFSA. EFSA reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors.Page 3 DEFINITION and DISTRIBUTIONAfrican Swine Fever (ASF) is caused by a complex DNA virus classified in the Asfarviridae family, genus Asfivirus, which infects different species of soft ticks and wild and domestic pigs. In the vertebrate hosts, the aetiological agent replicates preferentially in monocyte and macrophage cells and causes a range of symdromes and lesions from peracute to chronic and unapparent forms of disease.Actually, it is one of the most important diseases of domestic pigs, being of an exceptional seriousness, which can easily spread abroad and for which sanitary and socio-economical consequences have a significant impact on the national and international trade of animals and animal products. For this reason, it is listed as a Notifiable disease to the World Animal HealthOrganisation (OIE). At present there is no treatment or vaccine available, and control is based on rapid laboratory diagnosis and the enforcement of strict sanitary measures (Sanchez-Vizcaino, 2006). The virus has been maintained in African wild swine for a very long time: in eastern and southern Africa it is found in warthogs (Phacochoerus aethiopicus) and bushpigs (Potamochoerus porcus) also it is found in soft ticks of Ornithodoros genus. The wild swine in Africa can stay infected over a long period without showing any symptom of the disease -and thus can be considered as natural reservoirs of the disease, in contrast to the European wild boar (Sus scrofa) which appear to be highly susceptible (McVicar et al., 1988). The virus can be transmitted between O. moubata ticks by trans-stadial transmission as well as by sexual and trans-ovarian pathway, in contrast to O. erraticus in Europe where only trans...

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General morphology and capsid fine structure of African swine fever virus particles

Cited by 146 publications

References 29 publications

African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats

African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats

3.3 Afrikanische Schweinepest

Scientific review on African Swine Fever

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