The role of wastewater suspended solids in denitrification based on intracellular carbon storage was investigated in a biofilm sequencing batch reactor performing alternately anaerobic carbon storage and denitrification. Municipal wastewater as the feeding was compared with filtered wastewater and with acetate. The results show that the amount of PHA (polyhydroxyalkanoates) stored during a cycle was quite similar, irrespective of the substrate type used as feeding (acetate, real wastewater and real wastewater after filtration). PHA storage was limited even under excess chemical oxygen demand (COD) conditions, with a reducing power capacity enough for denitrification of only 25-26 mg/L N. However, when non-filtered wastewater was used, the denitrification capacity was about 50% higher (38 mg/L N) due to the contribution of entrapped suspended solids as the electron donor. In addition, the involvement of the hydrolyzed wastewater suspended solids resulted in a different PHA composition containing a much higher poly-3-hydroxyvalerate content.