General observations on the ingrowth of new blood vessels into standardized chambers in the rabbit's ear, and the subsequent changes in the newly grown vessels over a period of months

Clark, Eliot R.; Hitschler, William J.; Kirby‐Smith, H. T.; Rex, R. O.; Smith, Jay

doi:10.1002/ar.1090500203

Cited by 96 publications

(25 citation statements)

References 9 publications

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“…In addition the value for ingrowth of fibroblasts in rabbit ear chambers has been determined as 0.19 mm./day [Stearns, 1939] and an 'average' rate for blood capillaries in rabbit ear chambers as 0.22 mm./day . The question of asymmetry of repair tissue ingrowth in estimating growth rates, has been dealt with [Clark et al, 1931;Van Den Brenk, 1956]. It would appear reasonable to set the upper limit for the average rate of ingrowth of repair tissue into rabbit ear chambers at 0.24 mm./day and the lower limit at 0.09 mm./day or to 10.0 ,u/hr.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Wound Healing in Rabbit Ear Chambers, a Time Lapse Cinemicroscopic Study

Cliff

1965

Exp Physiol

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Wound healing in rabbit ear chambers was studied by time lapse cinemicroscopy.Endothelial cells for the growth of blood vessel sprouts arose in a zone proximal to the growing fringe both by the mitotic division of existing endothelium and by the conservation for re-use of the endothelium of resorbing blood vessels. The migration of endothelial cells distally along the luminal surfaces of the walls of circulating blood vessels, into the proximal ends of developing blood vessel sprouts, and along the mid part and finally into the tips of blood vessel sprouts was demonstrated. New lumen of sprouts was produced by the formation, enlargement and coalescence of loculated spaces between apposed layers of endothelium. This process was considered to reflect secretory activity of the endothelial cells. Anastomosis between sprouts involved similar lumen formation and the spatial rearrangement of endothelial cells.Evidence supporting the conversion of fibroblasts into smooth muscle cells was obtained. Growth of lymphatic sprouts was also studied. The virtual absence of locomotion by macrophages during wound healing was confirmed and a possible explanation proposed. Mathematical analysis of the data obtained from film analysis revealed that the curves for blood vessel endothelial migration and sprout growth and lymphatic sprout growth were all derivable from the same general pseudo generating mathematical function.

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Section: Discussionmentioning

confidence: 99%

Kinetics of Wound Healing in Rabbit Ear Chambers, a Time Lapse Cinemicroscopic Study

Cliff

1965

Exp Physiol

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“…ECs can sense their hemodynamic environment (9,10), and these forces can alter vascular structures (11)(12)(13). Increased capillary growth from sprouting vessels in frog larvae was observed with high flow, whereas capillaries regressed when flow ceased (14).…”

Section: Mt1-mmp Membrane Localization To Initiate Endothelial Sproutmentioning

confidence: 99%

Fluid Shear Stress and Sphingosine 1-Phosphate Activate Calpain to Promote Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) Membrane Translocation and Endothelial Invasion into Three-dimensional Collagen Matrices

Kang

Kwak

Kaunas

et al. 2011

Journal of Biological Chemistry

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Background: Wall shear stress (WSS) and sphingosine 1-phosphate (S1P) combine to promote endothelial sprouting and angiogenesis. Results: WSS and S1P activate calpains, and calpains are required for endothelial sprouting. Blocking calpains reduced membrane type-1 matrix metalloproteinase (MT1-MMP) membrane localization. Conclusion: Calpains regulate MT1-MMP membrane localization. Significance: These data uncover a new mechanism for controlling a key metalloproteinase in angiogenesis.

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“…Presumably the endothelium responds to x-ray injury by reparative growth in a non-specific way comparable to that following thermal burns, physical trauma, and infection. 19 Considerable edema occurred in the entire pouch from the 2nd to the 11th day after doses of 5,000 to 25,000 r. The epithelial tissue sloughed off and necrosis developed with 10,000 r and more. Necrosis occurred at 5,000 r when the entire cheek pouch was irradiated.…”

Section: Effects Of Irradiations On Circulationmentioning

confidence: 99%

Effect of X-irradiation and Beta Emanation on Circulation in the Hamster Cheek Pouch

Fulton

Lutz

Kagan

1956

Circulation Research

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The small venules of the hamster cheek pouch were rendered susceptible to hemorrhage by total body x-irradiation, cheek pouch x-irradiation and beta emanations from strontium-90 glass beads imbedded in the cheek pouch, as determined by negative pressure and moccasin venom tests. Petechiae were formed without vessel rupture and ceased without formation of platelet plug or fibrin clot. Increased susceptibility to petechiae preceded thrombocytopenia. Endothelial damage was indicated by increased leukocytic adhesiveness to walls of venules. Vasodilation, tortuosity, newlyformed capillaries, and occasional "sausage-shaped" segments of constriction in arterioles were characteristic vascular responses. Near death, erythrocytes circulated in aggregates varying from typical rouleau formation to discrete masses, resembling "sludged blood" but without formation of occluding thrombi.

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General observations on the ingrowth of new blood vessels into standardized chambers in the rabbit's ear, and the subsequent changes in the newly grown vessels over a period of months

Cited by 96 publications

References 9 publications

Kinetics of Wound Healing in Rabbit Ear Chambers, a Time Lapse Cinemicroscopic Study

Kinetics of Wound Healing in Rabbit Ear Chambers, a Time Lapse Cinemicroscopic Study

Fluid Shear Stress and Sphingosine 1-Phosphate Activate Calpain to Promote Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) Membrane Translocation and Endothelial Invasion into Three-dimensional Collagen Matrices

Effect of X-irradiation and Beta Emanation on Circulation in the Hamster Cheek Pouch

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