Greenberg, S.S., J. Paul, and B. Touhey: Hemodynamic profile of the new cardiotonic agent CK-2289 compared to milrinone and enoximone in the anesthetized dog. Drug Dev. Res. 23:109-126. 1991. We examined the hemodynamic profile of CK-2289, a new imidazolone developed for the treatment of congestive heart failure, in the normal pentobarbital-anesthetized dog. Mongrel dogs (10-18 kg) of either sex were anesthetized with pentobarbital sodium (35 mgikg, intravenously [i.v.]) and instrumented for routine hemodynamic measurements using an open-chest, artificially ventilated preparation. Intravenous administration of CK-2289 (3, 10, 30, and 100 pg/kg) to pentobarbital-anesthetized dogs increased left ventricular (LV) dP/ d T , , by 9, 26, 73, and 80%, respectively, and decreased mean arterial pressure (MAP) by 2, 6, 18, and 34%. CK-2289 was 2.7 times more potent as a positive inotropic agent than as a vasodilator. Heart rate (HR) increased by 2, 10, 18, and 28% after these doses of CK-2289, while left ventricular end diastolic pressure (LVEDP) decreased by 4.5 mmHg after the highest dose of compound. CK-2289 increased coronary blood flow (CBF) after each dose of compound. However, the increased CBF was less than that produced by milrinone for equivalent increases in LV dP/ dT, , , .Renal blood flow increased while vertebral blood flow was reduced after administration of the two highest doses of CK-2289. However, vertebral vascular resistance decreased slightly from control values. Milrinone and enoximone behaved qualitatively similar to, but were 6 and 27 times less potent than